Files in this Data Supplement:

• Supplemental Methods - (Microsoft Word) (39 kb)
• Table I - (Microsoft Word) (107 kb)
• Table II - (Microsoft Word) (25.5 kb)
• Table III - (Microsoft Word) (31.5 kb)
• Figure I - (TIF) (11.5 MB) Whole mount X-gal staining patterns of various organs of newborn mice reveal strong and homogeneous Cre activities in the lung. Lung (a-c), brain (d-f), mesenteric vessels (g-i), kidney (j-l), blood vessels in the thoracic wall (m-o), and liver (p-r) from control (a, d, g, j, m and p), L1cre(+);R26R (b, e, h, k, n and q), and B1cre(+);R26R (c, f, i, l, o and r) newborn pups were stained with the X-gal staining solution overnight. Blue regions represent the cells at which the cre recombinase is (was) expressed, except for the small intestine (g-i) and kidney (j-l) where endogenous â-galactosidase activities were present. X-gal staining pattern of the lung in L1cre;R26R (b) is the indistinguishable from that in B1cre mice (c). Brain is another organ where L1cre activity was readily detected at the same level as B1cre activity (e, f). X-gal staining patterns in mesenteric vessels, kidneys and blood vessels in the thoracic wall (as a representative of blood vessels in skeletal muscles) show that L1cre has patchy, mosaic cre activities in systemic vessels. Histological sections confirmed that most of X-gal positive cells are vascular endothelial cells (not shown). In the liver of L1cre mice, almost no X-gal-positive cells were detected.
• Figure II - (TIF) (4.69 MB) Expression of serotonin transporter and tenacin-C in vSMC layers of PH lungs. Immunostainings of control and mutant lungs with antibodies against serotonin transporter (5HTT; a-c) and tenascin-C (Tn-C; d-f) revealed elevated expression of both 5HTT (c) and Tn-C (f) in the vSMC layer of PH group samples compared to control (a and d) and N-PH group samples (b and e). Representative areas containing similar sized vessels were shown. Scale bars represent 25 μm.