Files in this Data Supplement:

• Supplemental Methods - (Microsoft Word) (44 kb)
• Figure I - (TIF) (845 kb) Representative in vivo rat heart MRI in two animals that received hMSCs. Short-axis image at 2 days after cell injection reveals a large signal void (arrow) at the injection site A: in an animal that received 7.5 × 10⁵ cells, B: in an animal that received 5 × 10⁵ cells. At 21 days (C,D), the infarcted myocardial wall is significantly thinned. C: A large signal void (arrow) persisted at the injection site 21 days after cell injection in the animal that received 7.5 × 10⁵ hMSCs, D: A smaller but clearly visible (arrow) signal void was seen at 21 days in the animal that received 5 × 10⁵ hMSCs.
• Figure II - (TIF) (882 kb) In vivo heart MRI of an animal that received pure Feridex intra-myocardially: A) at 2 days, B) at 7 days, C) at 14 days and D) at 21 days post-injection
• Figure III - (TIF) (0.98 MB) Representative histology of a rat heart harvested 21 days after myocardial infarction and hMSC injection, at the infarct border zone . A: Prussian Blue staining: a transmitted light image demonstrating a large number of iron-containing cells. B: CD-68 immunostaining: confocal imaging shows a large number of positive cells (macrophages), with a similar pattern to that seen with Prussian Blue positive cells.
• Movie I - (MPEG) (204 kb) In vivo rat cardiac MRI on a clinical 3T scanner (cine image, short axis). A large signal void was identified at 21 days after injection of xenogeneic CDCs.
• Movie II - (MPEG) (270 kb) In vivo rat cardiac MRI on a clinical 3T scanner (cine image, 4 chamber view). A large signal void was identified at 21 days after injection of xenogeneic MSCs in an infarcted animal.