Files in this Data Supplement:

• Supplemental Methods - (Microsoft Word) (67.5 kb)
• Movie I - (AVI) (2.31 MB) Platelet-derived SDF-1 mediates adhesion of CD34⁺ cells under dynamic conditions. Pretreatment of platelets with a blocking monoclonal antibody to SDF-1 attenuated the adhesion of CD34⁺ cells. Representative parts of two experiments are shown after perfusion of 8 minutes, one with control IgG and another with anti-SDF-1 mAb.
• Movie II - (Windows Media Video File) (6.17 MB) To evaluate the effect of the platelet-derived SDF-1 on CD34⁺ cell recruitment in vivo, the common carotid artery of wild-type C57BL/6J mice was injured by ligation and DCF stained CD34⁺ cells were intravenously injected and visualized using intravital fluorescence microscopy. Pre-infusion of anti-SDF-1 resulted in a decreased recruitment of progenitor cells in comparison with isotype control IgG1.
• Movie III - (AVI) (2.3 MB) Platelets adherent to endothelial cells recruit CD34⁺ cells towards the endothelium via platelet-derived SDF-1. Pretreatment of platelets with a blocking antibody to SDF-1 decreased the adhesion of CD34⁺ cells. Representative parts of two experiments are shown after perfusion for 8 minutes, one with control IgG and another with anti-SDF-1 mAb.
• Movie IV - (Windows Media Video File) (6.57 MB) To evaluate the biological significance of platelet-derived SDF-1 in the regulation of peripheral homing of CD34⁺ cells in microvasculature, fluorescent progenitor cells were infused before intestinal ischemia / reperfusion injury and visualized in the postischemic microcirculation by intravital fluorescence microscopy. Pre-infusion of anti-SDF-1 resulted in a decreased recruitment of progenitor cells in microvasculature in comparison with isotype control IgG1.