Files in this Data Supplement:

• Figures - (Microsoft PowerPoint file)(61 KB). Figure I. Plasma CHGA and catestatin. Inverse correlation of plasma CHGA and catestatin immunoreactivities, suggesting a spectrum of processing. CHGA was assayed by the CHGA116-439 epitope, while catestatin (CHGA352-372) was assayed by the CHGA361-372 epitope. Each subject had normal renal function. Figure II. Effect of H+-translocating ATPase inhibition on the secretagogue-stimulated release of CHGA from sympathoadrenal (PC12) cells. Cells were transfected with pCMV-CHGA-EAP (embryonic alkaline phosphatase reporter) and exposed for 22 h to the indicated concentrations of the V-ATPase (H+-ATPase) inhibitor bafilomycin A1. Cells were washed with secretion medium and subsequently exposed for 15 min to secretion medium alone (mock) or to 2 mM Ba2+. The enzymatic activity of CHGA-EAP in the extracellular medium and in the cell lysate was assayed by chemiluminescence. The sorting index of CHGA-EAP into the regulated secretory pathway was defined by SI=(Rs-Rc)/Rc; where Rs represents the secretion rate of the chimera in the presence of Ba2+ (regulated release), and Rc is the secretion rate of the chimera in absence of stimulation (constitutive release). Values are given as the means of triplicate determinations ± SEM. *: p<0.05, ***: p<0.001, ANOVA with Dunnett’s post-hoc test.
• Methods and Tables - (Microsoft Word file) (57 KB).