Files in this Data Supplement:

• Supplemental Methods - (Microsoft Word) (82 kb)
• Figure I - (TIF) (2.66 MB) Effects of progesterone on APD in two different estrous stages. At both the estrous (A) and the diestrous stages (B), progesterone at 100 nmol/L significantly shortened APD. There was no significant difference in fractional shortening of APD between at the estrous stage and at the diestrous stage (C).
• Figure II - (TIF) (2 MB) Effects of progesterone on the activation curve and the inactivation curve of I_Ca,L. A, Activation curves in the cAMP-stimulated condition (open circles), after application of progesterone at 40.6 nmol/L (closed circles), and its washout (cross symbols). Continuous lines are results of Boltzmann’s fitting in the form of:

  Normalized I_Ca,L = 1 – 1/1 + exp{(V – V_1/2)/k}

  where V is the test potential, V_1/2 is the voltage that I_Ca,L is half-maximally activated, and k is the slope factor. B, Inactivation curves in the cAMP-stimulated condition (open circles), after application of progesterone at 40.6 nmol/L (closed circles), and its washout (cross symbols). Continuous lines are results of Boltzmann’s fitting in the form of:

  Normalized I_Ca,L = 1/1 + exp{(V – V_1/2)/k}

  where V is the test potential, V_1/2 is the voltage that I_Ca,L> is half-maximally activated, and k is the slope factor.

• Figure III - (TIF) (3.22 MB) Signaling pathway for I I_Ca,L suppression under SNS stimulated conditions. A, B, Effects of carboxy-PTIO (A) or L-NIO (B). a, b, Representative recordings of I_Ca,L (a) and peak I_Ca,L density (b) just after establishment of whole-cell patch configuration (open symbols), after stabilization of effects of cAMP and okadaic acid (gray symbols), and after stabilization of effects of 100 nmol/L progesterone (black symbols) in cells pre-incubated with carboxy-PTIO (100 μmol/L, n=4) or L-NIO (1 μmol/L, n=7) for >15min. *P<0.05. Statistical significance was analyzed with an ANOVA followed by Bonferonni’s test.
• Figure IV - (TIF) (3.22 MB) Surface expression and co-localization of PgR and caveolin-3. Confocal images of guinea-pig ventricular myocytes in the absence (A) and presence (B) of progesterone (100 nmol/L), and negative control without primary antibodies (C). Left panels indicate PgR immunostaining images (green), middle panels indicate caveolin-3 immunostaining images (red), and right panels indicate overlay images.