Real-Time 2-Photon Imaging of Mitochondrial Function in Perfused Rat Hearts Subjected to Ischemia/Reperfusion
Circulation Matsumoto-Ida et al.
114: 1497
Data Supplement
Files in this Data Supplement:
- Movie I
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(QuickTime Movie) (2.95 MB) Z-stack movie reconstructed from 100 optical slices of TMRE-loaded perfused heart starting from the epicardial surface to 50 μm in depth.
- Movie II
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(QuickTime Movie) (1.31 MB) A 3-D processing movie of a single cardiomyocyte in the perfused heart.
- Movie III
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(QuickTime Movie) (389 KB) A time-lapse movie of the heart subjected to no-flow ischemia for 60 min (MI group).
- Movie IV
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(QuickTime Movie) (485 KB) A time-lapse movie of the heart subjected to no-flow ischemia for 30 min followed by reperfusion for 30 min (MI/R group).
- Movie V
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(QuickTime Movie) (770 KB) A time-lapse movie of the heart subjected to no-flow ischemia for 60 min. The movie is a part of experimental period from 37 min to 43 min.
- Movie VI
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(QuickTime Movie) (503 KB) A time-lapse movie of the heart treated with 0.2 μmol/L cyclosporin A, followed by 30 min of ischemia and 30 min of reperfusion (CsA 0.2 group).
- Movie VII
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(QuickTime Movie) (406 KB) A time-lapse movie of the heart treated with 1.0 μmol/L cyclosporin A, followed by 30 min of ischemia and 30 min of reperfusion (CsA 1.0 group).
- Movie VIII
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(QuickTime Movie) (586 KB) A time-lapse movie of the heart subjected to 3 cycles of 5 min ischemia and 5 min reperfusion, followed by 30 min of ischemia and 30 min of reperfusion (IPC group).