Files in this Data Supplement:

• Supplemental Methods - (Word) (97.5 KB)
• Figure I - (Power Point) (265 KB) Illustration of left coronary artery ligation. Representative picture of an infarcted heart after exposure and temporary dislocation via a small left thoracotomy to place a suture ligation at the distal 1/2 of the LAD (arrow) with 6.0 silk suture. LV: anterior wall of the left ventricle. LA: left atrium. Bar = 1 mm.
• Figure II - (Power Point) (75 KB) Quantification of total S100A1 protein levels in murine myocardium in the course of post-MI heart failure. A, Representative Western blot of rh-S100A1 protein standard curve (upper panel) and densitometric quantitative analysis (lower panel). Different amounts of purified monomeric S100A1 protein (1.25, 2.5, 5 and 10 ng) were loaded onto SDS-PAGE gel, and Western blots (n=5) were quantified by infrared-based densitometry. Positive linear correlation was observed between purified rh-S100A1 protein and densitometry. B, calculated amounts of total cardiac S100A1 protein levels for sham and infarcted NLC/WT, STG and SKO mice (n=6 animals in each group). *P<0.05 vs. sham NLC/WT.
• Figure III - (Power Point) (77.5 KB) Effect of differential cardiac S100A1 protein abundance on caspase-3 activity in the course of post-MI heart failure. Quantitative assessment of caspase-3 activity showed a significant increase in caspase-3 activity in infarcted hearts of all groups. SKO-MI hearts however, displayed a significantly higher rise in caspase-3 activity than NLC/WT-MI hearts. In contrast, post-MI related increase in caspase-3 activity was significantly blunted in STG-MI hearts compared with NLC/WT hearts. Values for each animal were assessed in triplets (n=5 animals in each group). *P<0.05 vs. sham NLC/WT, †P<0.05 vs. NLC/WT-MI 28d.
• Figure IV - (Power Point) (26 KB) Impact of β-adrenergic and adenylylcyclase stimulation on cardiac S100A1 expression in vitro. A, Time-dependent increase of cardiac S100A1 mRNA levels in NVCM's in response to Gs-coupled receptor agonist isoproterenol. B, Representative Western blot for S100A1 displays the rise of cardiac S100A1 protein levels in NVCM'S due to the stimulation with isoproterenol. Unchanged 18s and CSQ mRNA and CSQ protein abundance were used for mRNA and protein normalization, respectively. Densitometric values are given as arbitrary units and normalized to control. n=3 different NVCM preparations. *P<0.05 vs. control vs. Iso.