Files in this Data Supplement:

• Figure I - (Power Point) (7.25 MB) Increased balloon-induced neointima in FasL-deficient gld mice. Photomicrographs of carotid cross sections 14 days after injury show little neointima in WT, but enhanced lesion formation in gld mice. Neointimal and medial areas identified with DAPI staining/polarized light (upper panel) accurately correspond with areas measured by Elastica van Gieson (EVG) staining (lower panel) obtained in adjacent sections.
• Figure II - (Power Point) (13.5 MB) Balloon distension injury induces marked early medial SMC apoptosis. Photomicrographs of carotid cross sections from WT mice 1 hour after balloon distension injury (upper and middle panel) demonstrate abundant TUNEL-positive cells (middle, arrows); the nuclear stainings of the same sections using DAPI (right) show morphologic signs of apoptosis such as coalescence and fragmentation (arrows); adjacent sections stained with anti-ssDNA identify numerous apoptotic cells (arrows) correlating with many TUNEL-positive nuclei. In cross-sections of contralateral carotid arteries without injury (lower panel), neither TUNEL- nor anti-ssDNA positive cells can be detected; bar=100 μm for upper and lower panel, bar=50 μm for middle panel.