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• Figure I - (TIF) (1.81 MB). Confirmation of circadian oscillations by Q- PCR. (A) Bmal1 and FKBP506 oscillations observed by microarray were also observed to oscillate in a de novo set of experiments in aortic tissue. (B) Reverba, Wee1, and Npas2, though not detected on microarray chips were confirmed to oscillate by Q- PCR, as expected. (C) Synchronous circadian rhythmicity between the Q- PCR and Affymetrix data (from Figure 1C) was indicated by an improvement in fit, as measured by a negative change in the AIC statistic. All genes had synchronous cycles except Thra, for which a slight phase shift was noted. The synchrony was nonsignificant in genes for which only one Q-PCR replicate was performed (Claudin-5, PFK, and Scd2).
• Figure II - (TIF) (1.83 MB). The metabolism cassette of genes explicitly oscillate in aortic tissue. The twenty-two genes relating to metabolic function that oscillated in aortic tissue (glucose and lipid metabolism) were also assessed by global expression profiling in kidney, liver, and skeletal muscle by and clustered according to phase. Of the 22 genes that oscillated in aorta, only a few (shown also in red) also to exhibited circadian rhythmicity in the other respective tissues.
• Figure III - (TIF) (950 kb). Circadian analysis of MyoD expression in skeletal muscle and aorta. MyoD a candidate transcription factor that exhibited a common regulatory element among oscillating genes, did exhibit circadian expression in skeletal muscle (black line), but exhibited only a non-significant trend in aortic tissue (red line).