Files in this Data Supplement:

• Figure I - (JPEG) (921 kb). Typical raw data of the tetanic tension analysis. Panel A shows tension outputs before and after experimental muscle damage done to TA muscle of the same leg. Panels B and C show the comparison of tension outputs on operated and contralateral muscle 4 weeks after cell transplantation. Sk-34-0; freshly isolated Sk-34 cells, Control; media transplantation without cells). N=Newton.
• Figure II - (JPEG) (595 kb). Confirmation of GFP positive muscle fibers after transplantation. A: GFP positive fibers stained with anti-GFP and visualized by DAB. The reaction consisted of green fibers detected under fluorescent microscope using a triple-band filter (serial section in B). Fluorescent light-microscopic detection of GFP positive fibers using a single-band GFP filter (C, Olympus U-MGFPHQ) and a triple-band filter (D, Chroma U-N61000v2). Muscle fiber #1 shows a relatively green reaction compared to #4 in C, however, both #1 and #4 are negative for GFP in D, whereas #2 and #3 are true GFP positive fibers. Panels E and F shows the spectral analysis of the #1 - #4 muscle fibers in C and D using confocal laser scanning microscopy (Olympus, FV1000). True GFP positive fibers (#2 and #3) show a peak normalized intensity at a wavelength between 500-510 nm on XY- scan spectrogram (E), and the average emission intensity of these fibers is apparently higher than that of the #1 and #4 fibers (F). These data show that the correct detection of GFP positive fibers is possible under fluorescent light-microscopy using a triple-band filter. Bars = 10 µm.