Files in this Data Supplement:

• Figure I - (Microsoft PowerPoint) (396 kb). Quantitative SPECT image reconstruction methods algorithm using attenuation, collimator-detector response (CDR) and scatter corrections improved image contrast and resolution thereby yielding significantly better image quality and quantitative accuracy (bottom) as compared to the conventional filtered back projection method without any compensation (top). Thus, contamination of images with spill-over from liver uptake is avoided in the current animal model.
• Figure II - (Microsoft PowerPoint) (1.80 MB). Photomicrograph of lung (top left), liver (top right), spleen (bottom left), and heart (bottom right) demonstrating cell tracker orange positive cells in all organs indicating the presence of the exogenously fluorescently labeled mesenchymal stem cells in these organs.
• Movie I - (MPEG) (405 kb). Volume rendered movie of combined SPECT/CT acquired immediately after intravenous injection of labeled MSCs in a dog in which a myocardial infarction was created 72 hours previously. SPECT emission data are show as a red hot metal scale and CT data are shown as a greyscale. Initial MSC distribution is primarily to the lung. Increased MSC uptake in the left lung relative to the right lung can be appreciated reflecting the positioning of the dog in the scanner in left decubitus (i.e., left side down). No localized cardiac uptake is appreciated.
• Movie II - (AVI) (12.6 MB). Volume rendered movie of combined SPECT/CT acquired immediately after intravenous injection of labeled MSCs in a normal dog. SPECT emission data are shown as a red hot metal scale and CT data are shown as a greyscale. Initial MSC uptake to the lungs with enhanced uptake to the left lung relative to the right lung, due to positioning in left decubitus, is apparent. No uptake to the heart is appreciated.
• Movie III - (MPEG) (379 kb). Volume rendered movie of combined SPECT/CT acquired 24 hours after intravenous injection of labeled MSCs in a dog with a myocardial infarction. SPECT emission data are shown as a red hot metal scale and CT data are shown as a greyscale. Redistribution of the MSCs to the liver is appreciated at 24 hours post-injection with localized homing of the MSCs to the heart.
• Movie IV - (MPEG) (396 kb). Volume rendered movie of combined SPECT/CT acquired 24 hours after intravenous injection of labeled MSCs in a normal dog. SPECT emission data are shown as a red hot metal scale and CT data are shown as a greyscale. Redistribution of the MSCs to the liver is appreciated at 24 hours post-injection. No cardiac uptake is visible.
• Movie V - (MPEG) (498 kb). Volume rendered movie of combined SPECT/CT acquired 4 days after intravenous injection of labeled MSCs in a dog with a myocardial infarction. SPECT emission data are shown as a red hot metal scale and CT data are shown as a greyscale. MSC redistribution to the liver and bone marrow are appreciated as well as MSC homing to the apical heart or infarcted myocardium.
• Movie VI - (MPEG) (402 kb). Volume rendered movie of combined SPECT/CT acquired 5 days after intravenous injection of labeled MSCs in a normal dog. SPECT emission data are shown as a red hot metal scale and CT data are shown as a greyscale. MSC redistribution to the liver and bone marrow are visible. However, no MSC uptake to the heart is appreciated.