Files in this Data Supplement:

• Figure I - (Microsoft PowerPoint) (8.5 MB). Oneway Tukey Kramer HSD analysis of log10 β-gal activity of untreated, dog ATVRX with rAAV, dog ATVRX rAD tissues and muscles. Treated Limb denotes samples from tibialis anterior, gastrocnemius, soleus, biceps femoris, adductor longus, vastus medialis, vastus lateralis, and rectus femoris from limb undergoing infusion or the comparable muscles from the untreated limbs. Rest of Body denotes samples from heart, diaphragm, liver, left and right triceps, contralateral tibialis anteriror, gastrocnemius, and quadriceps.
• Figure II - (Microsoft PowerPoint) (19.2 MB). Tukey Kramer HSD Analysis of Individual Muscle Groups log10 β-gal activity after ATVRX treatment with rAAV or rAd. (A) rAAV, TA, (B) rAd, TA (C) rAAV, Gastroc (D) rAd, Gastroc (E) rAAV, Soleus (F) rAd, Soleus (G) rAAV, Adductor (H) rAd, Adductor (I) rAAV, B. Fem (J) rAd, B. Fem (K) rAAV, R. Fem (L) rAd, R. Fem (M) rAAV, V. Med (N) rAd, V. Med (O) rAAV, V. Lat (P) rAd, V. Lat.
• Figure III - (Microsoft PowerPoint) (9.5 MB). Oneway Tukey Kramer HSD analysis of log10 β-gal activity of rat untreated, No ATVRX and ATVRX with <50 torr of applied pressure. Treated Limb denotes samples from tibialis anterior, gastrocnemius, soleus, semimembranous, biceps femoris, adductor longus, and quadriceps from limb undergoing infusion or the comparable muscles from the untreated limbs. Rest of Body denotes samples from heart, lung, diaphragm, liver, kidney, triceps, biceps, contralateral tibialis anteriror, gastrocnemius, and rectus femoris.
• Figure IV - (Microsoft PowerPoint) (8.6 MB). Oneway Tukey Kramer HSD analysis of log10 β-gal activity of rat untreated, No ATVRX and ATVRX with 100 torr of applied pressure. Treated Limb denotes samples from tibialis anterior, gastrocnemius, soleus, semimembranous, biceps femoris, adductor longus, and quadriceps from limb undergoing infusion or the comparable muscles from the untreated limbs. Rest of Body denotes samples from heart, lung, diaphragm, liver, kidney, triceps, biceps, contralateral tibialis anteriror, gastrocnemius, and rectus femoris.
• Figure V - (Microsoft PowerPoint) (9.8 MB). Oneway Tukey Kramer HSD analysis of log10 β-gal activity of rat untreated, No ATVRX and ATVRX with 400 torr of applied pressure. Treated Limb denotes samples from tibialis anterior, gastrocnemius, soleus, semimembranous, biceps femoris, adductor longus, and quadriceps from limb undergoing infusion or the comparable muscles from the untreated limbs. Rest of Body denotes samples from heart, lung, diaphragm, liver, kidney, triceps, biceps, contralateral tibialis anteriror, gastrocnemius, and rectus femoris.
• Figure VI - (Microsoft PowerPoint) (8.41 MB). Oneway Tukey Kramer HSD analysis of log10 β-gal activity of rat untreated, No ATVRX, ATVRX with <50. 100 and 400 torr of applied pressure in isolated limbs, and ATVRX with 200 torr of applied pressure in isolated hemibody. Muscle samples from tibialis anterior, gastrocnemius, soleus, semimembranous, biceps femoris, adductor longus, and quadriceps from limb undergoing infusion or the comparable muscles from the untreated limbs. The isolated hemi-body group includes muscles from both limbs, including gluteus maximus, gluteus minimus, right and left psoas, and rectus abdominus.
• Figure VII - (Microsoft PowerPoint) (3.6 MB). Oneway Tukey Kramer HSD analysis of log10 β-gal activity of rat heart treated with anterograde infusion without ATVRX and ATVRX via retrograde coronary vein infusion at 60-80 torr.
• Figure VIII - (Zipped TIF file) (10.3 MB zipped; 82.1 MB unzipped). Oneway Tukey Kramer HSD analysis of log10 β-gal activity of rat quadriceps treated with AAV2/2.CMV.LacZ and AAV2/1.CMV.LacZ infusion with ATVRX at similar pressures.
• Figure IX - (Zipped TIF file) (9.7 MB zipped; 87.8 MB unzipped). Oneway Tukey Kramer HSD analysis of log10 β-gal activity of rat isolated limb and isolated hemibody treated muscle with ATVRX and tissue from same animals outside the isolated regions. A - Untreated Muscle from hindlimbs; B - Untreated Tissue outside of Isolation (Heart, Diaphragm, Lung, Kidney, Liver, Spleen, Testis); C - ATVRX treated with 0 minutes of Dwell time muscle; D - ATVRX treated with 0 minutes of Dwell time outside of Isolation (Heart, Diaphragm, Lung, Kidney, Liver, Spleen, Testis); E - ATVRX treated with 5 minutes of Dwell time muscle; F - ATVRX treated with 5 minutes of Dwell time outside of Isolation (Heart, Diaphragm, Lung, Kidney, Liver, Spleen, Testis); G - ATVRX treated with 15 minutes of Dwell time muscle; H - ATVRX treated with 15 minutes of Dwell time outside of Isolation (Heart, Diaphragm, Lung, Kidney, Liver, Spleen, Testis); I - ATVRX treated with 30 minutes of Dwell time muscle; J - ATVRX treated with 30 minutes of Dwell time outside of Isolation (Heart, Diaphragm, Lung, Kidney, Liver, Spleen, Testis); K - ATVRX treated Hemibody muscle; L - ATVRX treated Hemibody outside of Isolation (Heart, Diaphragm, Lung, Kidney, Liver, Spleen, Testis).
• Figure X - (Microsoft PowerPoint) (7 MB). ATVRX Treated Rat Tissue Slides: (A) Gastrocnemius (100X), (C) Quadriceps (40X), (D) Glueteus Maximus (100X).
• Figure XI - (Microsoft PowerPoint) (4.66 MB). Comparison of log10 β-gal activities between different AAV pseudotypes (AAV2/2.CMV.LacZ or AAV2/1.CMV.LacZ) at varying pressures of ATVRX delivery in (A) quadriceps and (B) gastrocnemius.
• Figure XII - (Microsoft PowerPoint) (10 MB). (A) Varying muscles from canine left hindlimb infused with rAAV with ATVRX and the unifused right limb from the same dog stained with x-gal to detect lacZ expression. Tissue from the same treated hindlimb at higher magnification: (B) Vastus Medialis (40X), (C) Adductor Longus (40X), (D) Biceps Femoris (100X), (E) Soleus (100X)
• Table I - (Zipped TIF file) (4 MB zipped; 50.9 MB unzipped). Raw β-gal activity in R.L.U./µg protein from rat isolated limb studies.
• Table II - (Zipped TIF file) (8 MB zipped; 71.4 MB unzipped). Log10 β-Gal activity of tissues after ATVRX infusion at varying dwell times in isolated limb infusions at 100 torr and in isolated hemibody infusions at 200 torr with 30 minutes(‘) of dwell time.
• Table III - (Zipped TIF file) (5.5 MB zipped; 90.1 MB unzipped). Raw β-gal activity in R.L.U./µg protein from dog studies.