Files in this Data Supplement:

• Expanded Methods - (Microsoft Word) (29.0 kb).
• Figure - (Microsoft PowerPoint) (409 kb). (A) Contractile protein solubilization efficiency. 15% SDS gel electrophoresis showing extraction of myocardial proteins in the rehydration buffer used for 2-D electrophoresis. Proteins were identified using appropriate standards from pig heart. (B) Reproducibility of protein loading in 2-D gels. Densitometric analysis of actin spot intensities from heart extracts separated by 2-D gel electrophoresis. IPG strips with 3-6 pH gradient for the first dimension and 8-16% polyacrylamide gel gradient for the second dimension were used. 100 of μg total proteins were loaded per gel.. The inserts show representative actin spots within the 2-D gel from each group. The border of the actin spots (dark line around the spot) was determined by using the automatic spot contour tool provided by PDQuest. The lower panel shows the quantitative analysis of actin spot intensities. (C) Reproducibility of protein spot quantification between gels. Correlation of spot intensities from 2-D gel electrophoretic analysis of Aerobic control hearts extracts. Each graph represents the comparison of spot intensities of 40-50 protein spots meeting our inclusion criteria as analysed between two different gels within the same experimental group.