Figure 2. Macrophage-derived FCs isolated from
subcutaneous granuloma. Top, Cells isolated from subcutaneous granuloma
of hypercholesterolemic rabbits are identified by
immunofluorescence as macrophages (RAM-11
antibody). Inset, Negative control for immunostaining
in which primary antibody (Ab) was eliminated. Middle, Intracellular
lipid in macrophages from hypercholesterolemic
rabbits is revealed by staining with Nile red. Inset, Nile red staining
of macrophages (M
) from normocholesterolemic
rabbits. Bottom, Macrophage-derived FCs of granuloma
express stromelysin (MMP-3). Nuclei are counterstained with
bisbenzamide. Magnification x400.
