doi: 10.1161/CIRCULATIONAHA.105.000521
(Circulation. 2006;114:I-108 – I-113.)
© 2006 American Heart Association, Inc.
Cell Transplantation and Tissue Engineering |
Skeletal Myoblast Transplantation in Ischemic Heart Failure
Long-Term Follow-Up of the First Phase I Cohort of Patients
From Université Paris-Descartes (A.A.H., A.A., E.A., E.M., E. Mousseaux, M.D., P.M), Faculté de Médecine; INSERM U 633, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Departments of Cardiology, Imaging and Cardiovascular Surgery, Paris, France; Assistance Publique Hôpitaux de Paris (J.-P.M.), Hôpital Saint-Louis, Cell therapy laboratory, Paris, France; INSERM U 582 (J.-T.V., K.S.), Institut de Myologie, Paris, France; INSERM (S.P.), Clinical Investigation Center 92010, Paris, France; Université Paris-Descartes (D.D.), Faculté de Médecine, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Department of Cardiology, Paris, France.
Correspondence to Albert A. Hagège, Cardiology Department, Hôpital Européen Georges Pompidou, 20 Rue Leblanc, 75015 Paris, France. E-mail albert.hagege{at}egp.ap-hop-paris.fr
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Abstract |
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Background— Skeletal myoblast (SM) transplantation (Tx) in a post-myocardial infarction (MI) scar experimentally improves left ventricular (LV) ejection fraction (EF). Short-term follow-up (FU) studies have suggested that a similar benefit could clinically occur despite an increased risk of LV arrhythmias.
Methods and Results— We report the long-term FU of the first worldwide cohort of grafted patients (n =9, 61.8±11.6 years, previous MI, EF 
35%) operated on (autologous SM Tx and bypass surgery) in 2000 to 2001 and evaluated before Tx, at 1 month (M1) and at a median FU of 52 (18 to 58) months after Tx (37 patient-years). NYHA class improved from 2.5±0.5 to 1.8±0.4 at M1 (P=0.004 versus baseline) and 1.7±0.5 at FU (P=not significant versus M1; P=0.0007 versus baseline). EF increased from 24.3±4% to 31±4.1% at M1 (+28%, P=0.001 versus baseline) and remained stable thereafter (28.7±8.1%, +18% versus baseline). There were 5 hospitalizations for heart failure in 3 patients at 28.6±9.9 months, allowing implant in 2 patients with a resynchronization pacemaker. An automatic cardiac defibrillator (ACD) was implanted in 5 patients for nonsustained (n =1) or sustained (n =4) ventricular tachycardia at 12.2±18.6 (1 to 45) months. Despite a beta-blocker/amiodarone combination therapy, there were 14 appropriate shocks for 3 arrhythmic storms in 3 patients at 6, 7, and 18 months after ACD implantation.
Conclusions— In this cohort of severe heart failure patients both clinical status and EF stably improve over time with a strikingly low incidence of hospitalizations for heart failure (0.13/patient-years) and the arrhythmic risk can be controlled by medical therapy and/or on-request ACD implantation.
Key Words: follow-up studies • heart failure • myocardial infarction • skeletal myoblasts • transplantation
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Introduction |
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Cellular transplantation is receiving a growing interest as a mean of partially compensating for the loss of cardiomyocytes after myocardial infarction (MI).1 Among the candidate cells, skeletal myoblasts, which are responsible for skeletal muscle formation and repair, have been widely investigated because they feature clinically attractive characteristics (ease of procurement, autologous origin, marked growth potential, myogenic commitment, resistance to ischemia).2,3 Myoblast transplantation in a post-MI scar experimentally improves left ventricular (LV) ejection fraction (EF)4,5 and short-term follow-up. Phase I surgical studies6–9 of patients with ischemic cardiomyopathy and concomitant indication to coronary artery bypass graft (CABG) surgery have suggested that they could incur a similar benefit despite an increased risk of ventricular arrhythmias. However, if short-term results of the procedure are encouraging, long-term follow-up (FU) of a homogeneous cohort of patients is lacking. We report here the long-term FU of the first worldwide cohort of grafted patients operated on in a single center in 2000 to 2001.
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Materials and Methods |
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Study Group
Eligibility for the procedure was based on: (1) systolic LV dysfunction reflected by an echocardiographic LVEF
35%; (2) history of MI with a residual akinetic and nonviable scar, as demonstrated by the lack of response to low-dose dobutamine echocardiography and of metabolic activity on fluorine-18 fluorodeoxyglucose positron emission tomography; and (3) indication for concomitant CABG in remote areas (ie, different from the transplanted area). Patients with significant mitral regurgitation (MR) (>2+ on a scale of 4+ by extension of the color jet within the atrium) or poor echogenicity were excluded. The protocol was approved by the French Regulatory Health Authorities and an independent Ethics Committee. Patients were included after having given their informed written consent.
Staging of the Procedure
The procedure was described previously.6 From a 10- to 15-gram biopsy of the vastus lateralis muscle, a 2- to 3-week cell culture was started using Good Manufacturing Practices grade products according to the French Regulatory Agency requirements. The cell-containing suspension was transferred into sterile syringes and, on completion of CABG anastomoses in nontransplanted areas, 4 to 8 mL were injected in 27 to 57 sites within and around the scar. The areas of injection were drawn on a map based on the echocardiographic LV segmentation and subsequently used to target echocardiographic analysis of functional changes ascribed to cell transplantation.
Predischarge Evaluation
It has previously been reported in detail.6 Briefly, there were no early postoperative complications related to muscular biopsy or cell transplantation, particularly no bleeding, infection, or clinically manifest embolic episodes. Patient 5 died within the first 24 hours after transplantation from a mesenteric infarction, leaving 9 patients available for further evaluation.
Long-Term FU
Long-term FU included repeat (4 per year) clinical (New York Heart Association [NYHA] functional class, hospitalizations for congestive heart failure [CHF] or death), rhythmologic (24-hour Holter electrocardiogram monitoring and/or implantable automatic cardiac defibrillator [ACD] interrogations), and echocardiographic examinations.
Echocardiographic studies were conducted according to a standard protocol. Transthoracic 2-dimensional examinations used fundamental and second-harmonic imaging. Global LVEF (%) was calculated from measurements of LV end-diastolic (LVEDV) and LV end-systolic (LVESV) volumes using the biplane Simpson rule. Regional contractile function was assessed semi-quantitatively after division of the LV into 16 segments, as recommended by the American Society of Echocardiography.10 Care was taken to optimize endocardial visualization on 3 parasternal LV short-axis views (at the base, mid-ventricular, and apex levels) and apical 2-, 3-, and 4-chamber views. The baseline assessment was performed within 1 month before surgery, off episodes of cardiac failure, at rest. Thickening of each segment within the grafted area was visually graded (as dyskinetic, akinetic, severely hypokinetic, moderately hypokinetic, or normal) and serial changes were evaluated semi-quantitatively and classified as unchanged, improved (
1 grade) or worsened (1 grade). These grafted areas were localized by reference to the anatomic diagram established intraoperatively and depicting the sites of injections. Image analysis was performed by a single senior investigator, blinded to the timing of echocardiographic data, number and location of CABG, and sites of injections. Excellent reproducibility of segmental thickening evaluation was previously reported in the same cohort of patients.6
Statistics
All results are reported as the mean±SD. Safety and efficacy were evaluated between the first and second months after procedure (M1) and at the end of FU (October 1, 2005) and compared with baseline data (collected within 1 month before the procedure) using a paired nonparametric Wilcoxon test with P<0.05 as the limit of significance.
The authors had full access to the data and take full responsibility for their integrity. All authors have tread and agree to the manuscript as written.
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Results |
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Study Group
Nine male patients formed the study group (Table 1). Age of MI ranged from 3 months to 19 years. Its location was anterior (5), posterior (3), or postero-lateral (1). Four patients were in NYHA functional class II and 5 in class III. Patients received 2 (n =8) to 3 (n =1) bypass grafts. Patient 1 died from a stroke at 17.5 months FU and pathologic examination of the explanted heart showed in-scar clusters of myotubes.11 Thus, 9 patients were evaluated before transplantation (baseline) and at M1, and 8 were available for long-term FU. All received optimal medical therapy before surgery, including diuretics, angiotensin-converting enzyme (ACE) inhibitors, and beta-blockers at maximal tolerated doses and these treatments were maintained at follow-up.
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FU
Mean FU was 49.4 months (median, 52 [18–58]), corresponding to 37 patient-years.
Clinical Data
NYHA class improved from 2.5±0.5 before surgery to 1.8±0.4 at M1 (P=0.004 versus baseline) and 1.7±0.5 at end of FU (P=1.0 versus M1; P=0.0007 versus baseline) (Table 2). There were 5 hospitalizations for CHF in 3 patients at 28.6±9.9 (range, 13 to 71) months, corresponding to 0.13/patient-years. Because of persistent symptoms, 2 patients (7 and 9) were implanted with a ventricular resynchronization pacemaker, whereas a third one (2) received ACD with a resynchronization function on the referring physician’s request.
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Echocardiography
There was no statistical difference between systolic blood pressures measured before each ultrasonic examinations and MR remained minimal or mild. LVEDV remained unchanged over time (201±56 mL at baseline, 209±61 mL at M1, and 215±72 mL at FU, P>0.4 for all comparisons). At these time points, LVEF increased from 24.3±4% (18 to 31) to 31±4.1% (25 to 35) at M1 (+28%, P=0.001 versus baseline) and remained stable thereafter (28.7±8.1% [17 to 40] at FU [+18% relative to baseline, P=0.64 versus M1, P=0.24 versus baseline]) (Figure 1). A high interindividual variability was observed as 3 patients continuously improved over time, whereas 3 showed a late deterioration (below baseline values) after an initial increase. According to the surgical scheme of the grafted segments, a total of 24 in-scar akinetic nonviable segments were treated (Table 2). At 1 month, 14 of them (58.3%) demonstrated a new-onset echocardiographic systolic shortening (P=0.015), whereas at end of FU only 8 (33.3%) remained improved (P=0.03 versus baseline, P=0.15 versus M1) (Figure 2). The corresponding number of improved patients were 7 of 9 at M1 and 6 of 8 (75%) at end of FU.
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Arrhythmias
An ACD was implanted in 5 patients at 12.2±18.6 (1 to 45) months of FU (Table 3). Indications were asymptomatic nonsustained ventricular tachycardias (SVT) at 45 months after procedure in patient 2 (with no firing during the next year of FU) or SVT within the first month after the procedure in 4 patients (4, 6, 7, and 8). Three of them had evidence for ventricular hyperexcitability (Lown grade 2 to 3) on preoperative Holter recordings. Except for 1 severe syncopal form (patient 4), which necessitated invasive electrophysiologic stimulation for recovery of sinus rhythm, all other episodes were clinically well-tolerated and successfully managed by ß-blockers and amiodarone. Despite this combination therapy, late SVTs were observed in patients 4 (at 5 months, motivating ACD implantation, and at 7 months, with no further SVT recorded over the next 49 months), 6 (at 8 months, leading to ACD implantation, with no SVT recorded over the next 45 months), and 7 and 8 who had implantation before discharge (at 18 and 6 months of FU, with no recurrence during the next 34 and 46 months, respectively). Three of these late arrhythmias featured an arrhythmic storm (patients 4, 7, and 8), necessitating a total of 14 ACD firings. In 2 of these patients, there were 6 nonappropriate shocks for atrial arrhythmias or sinus tachycardia.
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Discussion |
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More than 15 years of experimental data have suggested a beneficial effect of skeletal myoblast transplantation within injured hearts.1–3 Since the first operation performed in June 2000,12 3 surgical studies of small cohorts of patients have combined autologous myoblast transplantation and CABG in post-MI patients with low LVEF.7–9 These phase I studies were not designed or powered to demonstrate the efficacy of the technique, but rather its feasibility and safety. However, the current study differs noticeably from previous publications.
Although most of the previous studies were multicentric, with patients occasionally undergoing additional procedures (like aneurysmectomy),8,9 the current study includes a very homogeneous cohort of patients from a single center. Second, the number of injected and viable cells is markedly higher than in the previously published articles (mean 953 versus <300 million cells), as is the purity of the injected solution, which may be clinically relevant in view of the suggested link between the number of injected cells and the efficacy and arrhythmogenicity of the procedure.6 Third, the FU is the longest ever reported (up to 5 years, versus 3, 12, and 24 months),7–9 allowing to confirm the absence of tumorigenicity of the procedure and to systematically check for long-term arrhythmogenicity. The short-term occurrence of SVT has been highlighted since the first clinical study6 but, so far, the long-term risk of the procedure has remained unknown.
In the current study, patients with SVT at long-term FU always exhibited SVT during the first postoperative month. Except for patient 4, arrhythmias were controlled by drug regimen and/or on-request ACD implantation. Presence of short-term non-SVT was not predictive of future events. Late arrhythmic storms have been observed until 18 months after grafting and were correctly managed by multiple ACD firings. Even if a direct link between the procedure and arrhythmias remains speculative, because of the underlying arrhythmogenic substrate inherent to CHF, absence of electrical coupling, and a resulting inhomogeneous electrical propagation might account for reentry circuits.13 Of note, no SVT was observed among those patients with continuously improved EF, whereas 3 of 4 patients who exhibited SVT showed a marked decrease in EF from short-term to long-term, raising the possibility that those arrhythmic events were not procedure-related but reflected CHF evolution, graft closure, or progression of atherosclerosis in native vessels (a control coronary angiogram was only performed after the first SVT and was not repeated thereafter). Because an equivalent proportion (31%) of patients issued from a similar (SCD-HeFT) population (age, 60 years; NYHA II/III; EF 35%; FU, 45.5 months) and systematically implanted with an ACD received shocks from their device,14 it is clear that only the ongoing randomized placebo-controlled MAGIC trial, which entails implantation of a defibrillator in all patients, will allow to support or refute a causal relationship between myoblast implantation and ventricular arrhythmias.
The current study includes 3 data point functional evaluations, adding more longitudinal information than previous publications, particularly concerning the contrast between improved EF at short-term, as previously shown (+6.8% to +18%),6–9 and the decreased beneficial effect at long-term, suggesting a potential loss of efficacy over time. There was, however, a marked interindividual variability, because one-third of the patients exhibited an initial increase followed by a decrease below the pre-transplant values. While improved EF might be consecutive only to associated CABG surgery, it is the only study in which the grafted scar was not bypassed. This is particularly relevant to an accurate interpretation of thickening recovery in the grafted areas and allows a more reliable "proof of concept" demonstration, because contractility of nonviable and nonrevascularized transmural infarcted areas is uncommon after revascularization of remote myocardial territories. Although more than half of the grafted segments demonstrated systolic thickening recovery at short-term, this percentage slightly decreased to 33% at long-term. This is consistent with a high rate of cell death, which is incompletely compensated for by in vivo proliferation within the scar.15 Cell manipulations to increase graft proliferation, survival, and electrical coupling with the host tissue16 look to further enhance safety and efficacy of myoblast engraftment.17 Nevertheless, the majority of the patients still exhibited at least 1 improved segment at end of FU. That was consistent with serial magnetic resonance imaging (MRI) studies performed in 2 non-ACD patients (Figure 2) and position emission tomography data showing recovery of metabolic activity within the grafted scar.12 Such an improvement has been also reported in animals using tissue Doppler imaging5 or MRI18 techniques and in humans,6–8 despite the fact that the grafted myotubes remain isolated from the host tissue and do not express connexin-43, thereby precluding synchronous contraction13 and rather suggesting myoblast-mediated paracrine signaling.6
Finally, functional class stably improved over time after the procedure, along with a strikingly low incidence of hospitalizations for congestive heart failure (CHF) (0.13 patient-years) as compared with similar CHF populations.19 The anti-remodeling effect of the procedure, suggested by animal experiments,5,18 was confirmed by the absence of further LV dilatation over time, possibly caused by the scaffolding properties of the graft.
Limitations
The improvement in LV function was not the primary end point of this study, which was designed to rather address the long-term safety of the procedure, particularly with regard to tumor development and arrhythmias that might have been related to the high number of injected cells. Echocardiography was the only method used to track the changes in systolic function after surgery; MRI, assumed to be more accurate, was excluded because of ACD implantation in several patients. However, the consistency of ultrasonic measurements of EF and segmental scoring for 2 examinations performed by the same observer reached 99% in the previously published phase I study.6 Second, the associated CABG surgery could clearly confound interpretation of functional outcomes, although this study, in contrast to others,7–9 entailed exclusive revascularization of the noncell-transplanted segments. Finally, it is clear that improved LV function does not necessarily equate myocardial regeneration and as such the present results still fail to provide a mechanistic insight of myoblast transplantation.
In conclusion, the present long-term study confirms that autologous skeletal myoblast transplantation is a feasible and relatively straightforward procedure. In a very homogeneous cohort of severe CHF patients, both functional class and ventricular function stably improved over time, whereas the incidence of hospitalizations for CHF remained strikingly low. In these patients receiving the highest number of cells ever injected, severe arrhythmias may occur at long-term, but they can be controlled by a prophylactic drug regimen and/or on-request ACD implantation. Larger randomized, placebo-controlled, double-blind studies, such as the MAGIC trial that we are currently conducting, should soon generate meaningful safety and efficacy data allowing to accurately assess the risk-to-benefit ratio of the procedure.
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Acknowledgments |
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Sources of Funding
This study was supported by a French Public Institution, the Département de la Recherche Clinique et du Développement Ile de France, Assistance Publique - Hôpitaux de Paris, Paris, France.
Disclosures
This study was promoted by the Département de la Recherche Clinique et du Développement Ile de France, Assistance Publique Hôpitaux de Paris, Paris, France (http://www.drrc.aphp.fr/), with the promotion number P 990503. Since completion of this phase I study, Genzyme has provided research and consulting support, but there is no conflict relevant to the present work, which was exclusively academia-driven and funded by a public grant.
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Footnotes |
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Presented at the American Heart Association Scientific Sessions, Dallas, Tex, November 13–16, 2005.
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References |
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1. Laflamme MA, Murry CE. Regenerating the heart. Nat Biotechnol. 2005; 23: 845–856.[CrossRef][Medline] [Order article via Infotrieve]
2. Menasche P. Myoblast transfer in heart failure. Surg Clin North Am. 2004; 84: 125–139.[CrossRef][Medline] [Order article via Infotrieve]
3. Murry CE, Wiseman RW, Schwartz SM, Hauschka SD. Skeletal myoblast transplantation for repair of myocardial necrosis. J Clin Invest. 1996; 98: 2512–2523.[Medline] [Order article via Infotrieve]
4. Taylor DA, Atkins BZ, Hungspreugs P, Jones TR, Reedy MC, Hutcheson KA, Glower DD, Kraus WE. Regenerating functional myocardium: improved performance after skeletal myoblast transplantation. Nat Med. 1998; 4: 929–933.[CrossRef][Medline] [Order article via Infotrieve]
5. Ghostine S, Carrion C, Souza LC, Richard P, Bruneval P, Vilquin JT, Pouzet B, Schwartz K, Menasche P, Hagege AA. Long-term efficacy of myoblast transplantation on regional structure and function after myocardial infarction. Circulation. 2002 Sep 24; 106 (12 Suppl 1): I131–I136.
6. Menasche P, Hagege AA, Vilquin JT, Desnos M, Abergel E, Pouzet B, Bel A, Sarateanu S, Scorsin M, Schwartz K, Bruneval P, Benbunan M, Marolleau JP, Duboc D. Autologous skeletal myoblast transplantation for severe postinfarction left ventricular dysfunction. J Am Coll Cardiol. 2003; 41: 1078–1083.
7. Siminiak T, Kalawski R, Fiszer D, Jerzykowska O, Rzezniczak J, Rozwadowska N, Kurpisz M. Autologous skeletal myoblast transplantation for the treatment of postinfarction myocardial injury: phase I clinical study with 12 months of follow-up. Am Heart J. 2004; 148: 531–537.[CrossRef][Medline] [Order article via Infotrieve]
8. Herreros J, Prosper F, Perez A, Gavira JJ, Garcia-Velloso MJ, Barba J, Sanchez PL, Canizo C, Rabago G, Marti-Climent JM, Hernandez M, Lopez-Holgado N, Gonzalez-Santos JM, Martin-Luengo C, Alegria E. Autologous intramyocardial injection of cultured skeletal muscle-derived stem cells in patients with non-acute myocardial infarction. Eur Heart J. 2003; 24: 2012–2020.
9. Dib N, Michler RE, Pagani FD, Wright S, Kereiakes DJ, Lengerich R, Binkley P, Buchele D, Anand I, Swingen C, Di Carli MF, Thomas JD, Jaber WA, Opie SR, Campbell A, McCarthy P, Yeager M, Dilsizian V, Griffith BP, Korn R, Kreuger SK, Ghazoul M, MacLellan WR, Fonarow G, Eisen HJ, Dinsmore J, Diethrich E. Safety and feasibility of autologous myoblast transplantation in patients with ischemic cardiomyopathy: four-year follow-up. Circulation. 2005; 112: 1748–1755.
10. Schiller NB, Shah PM, Crawford M, De Maria A, Devereux R, Feigenbaum H, Gutgesell H, Reicheck N, Sahn D, Schnittger I, Silverman NH, Tajik AJ. Recommendations for quantitation of the left ventricle by two-dimensional echocardiography. Am Society of Echocardiography Committee on Standards, Subcommittee on quantitation of two-dimensional echocardiograms. J Am Soc Echocardiogr. 1989; 2: 358–367.[Medline] [Order article via Infotrieve]
11. Hagege AA, Carrion C, Menasche P, Vilquin JT, Duboc D, Marolleau JP, Desnos M, Bruneval P. Viability and differentiation of autologous skeletal myoblast grafts in ischaemic cardiomyopathy. Lancet. 2003; 361: 491–492.[CrossRef][Medline] [Order article via Infotrieve]
12. Menasche P, Hagege AA, Scorsin M, Pouzet B, Desnos M, Duboc D, Schwartz K, Vilquin JT, Marolleau JP. Myoblast transplantation for heart failure. Lancet. 2001; 357: 279–280.[CrossRef][Medline] [Order article via Infotrieve]
13. Leobon B, Garcin I, Menasche P, Vilquin JT, Audinat E, Charpak S. Myoblasts transplanted into rat infarcted myocardium are functionally isolated from their host. Proc Natl Acad Sci U S A. 2003; 100: 7808–7811.
14. Bardy GH, Lee KL, Mark DB, Poole JE, Packer DL, Boineau R, Domanski M, Troutman C, Anderson J, Johnson G, McNulty SE, Clapp-Channing N, Davidson-Ray LD, Fraulo ES, Fishbein DP, Luceri RM, Ip JH for the Sudden Cardiac Death in Heart Failure Trial (SCDE-HeFT) Investigators. Amiodarone or implantable cardioverter-defibrillator for congestive heart failure. N Engl J Med. 2005; 352: 225–237.
15. Maurel A, Azarnoush K, Sabbah L, Vignier N, Le Lorc’h M, Mandet C, Bissery A, Garcin I, Carrion C, Fiszman M, Bruneval P, Hagege A, Carpentier A, Vilquin JT, Menasche P. Can cold or heat shock improve skeletal myoblast engraftment in infarcted myocardium? Transplantation. 2005; 80: 660–665.[CrossRef][Medline] [Order article via Infotrieve]
16. Azarnoush K, Maurel A, Sebbah L, Carrion C, Bissery A, Mandet C, Pouly J, Bruneval P, Hagege AA, Menasche P. Enhancement of the functional benefits of skeletal myoblast transplantation by means of coadministration of hypoxia-inducible factor 1alpha. J Thorac Cardiovasc Surg. 2005; 130: 173–179.
17. Abraham MR, Henrikson CA, Tung L, Chang MG, Aon M, Xue T, Li RA, O’ Rourke B, Marban E. Antiarrhythmic engineering of skeletal myoblasts for cardiac transplantation. Circ Res. 2005; 97: 159–167.
18. van den Bos EJ, Thompson RB, Wagner A, Mahrholdt H, Morimoto Y, Thomson LE, Wang LH, Duncker DJ, Judd RM, Taylor DA. Functional assessment of myoblast transplantation for cardiac repair with magnetic resonance imaging. Eur J Heart Fail. 2005; 7: 435–443.[CrossRef][Medline] [Order article via Infotrieve]
19. Zannad F, Briancon S, Juilliere Y, Mertes PM, Villemot JP, Alla F, Virion JM. Incidence, clinical and etiologic features, and outcomes of advanced chronic heart failure: the EPICAL Study. J Am Coll Cardiol. 1999; 33: 734–742.
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