Files in this Data Supplement:

• Supplemental Methods - (Microsoft Word) (41.0 kb).
• Figure I - (TIF) (2.63 MB). Biplane echocardiographic color-Doppler images acquisition and initial processing. Panels A-C: example of raw individual frames extracted from biplane sequences obtained at rotational positions corresponding to 0, 60 and 120 degrees, respectively. During each sequence acquisition, the left half screen shows a constant 4-chamber view used as reference. The right half screen simultaneously displays the image at a particular rotation, as shown by the navigation icon in the center of the screen. Panel D, results of image decoding and registration of the three frames shown in panels A-C. The red-green-blue components of each color pixel in the image have been decoded to obtain their respective velocity value. Raw velocities have been dealiased and each image has been then spatially registered according to its rotational position. Using this methodology, 36 sequences (one beat) were acquired and processed from 0 to 180 degrees, in steps of 5 degrees, to obtain the full 3D + T + velocity dataset.
• Figure II - (TIF) (214 kb). Three-dimensional spatial position of the jet core and its bilinear approximation. For the instant of peak ejection flow velocity, the core position of the jet is shown in blue, from the LV apex (low z values) to the LVOT (high z values). Although a certainly non linear trajectory is found at the LV apical level, it is almost irrelevant for the estimation of EIVPDs, because flow velocity is very low at this location. The bilinear approximation to the jet core is shown in red, estimated by orthogonal regression of the core position data. See text for details.
• Movie - (AVI) (454 kb). Three-dimensional representation of the flow velocity at the LVOT level. The x and y axes represent sample volume position in the transversal plane whereas the z and color axes represent flow velocity. Trivial aortic regurgitation is present during diastole. For the purpose of visualization, flow velocities (in blue) are shown with an opposite sign.