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(Circulation. 2004;109:3171-3175.)
© 2004 American Heart Association, Inc.
Clinical Investigation and Reports |
From the Heart Institute (S.M., V.G., M.S., R.B., I.G., I.N., H.P., H.H.), Institute of Thrombosis and Hemostasis (B.S., D.V.), and Goldschleger Eye Research Institute (N.S.), Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Correspondence to Hanoch Hod, MD, Director, ICCU, Heart Institute, Sheba Medical Center, Tel Hashomer 52621, Israel. E-mail hod{at}netvision.net.il
Received October 20, 2003; de novo received December 14, 2003; revision received February 24, 2004; accepted March 16, 2004.
| Abstract |
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Methods and Results The antiplatelet effect of clopidogrel was studied prospectively in 60 consecutive patients who underwent primary angioplasty (percutaneous coronary intervention [PCI]) with stenting for acute ST-segmentelevation myocardial infarction (STEMI) to determine whether variability in response to clopidogrel affects clinical outcomes. Patients were stratified into 4 quartiles according to the percentage reduction of ADP-induced platelet aggregation. Although patients in the first quartile were resistant to the effects of clopidogrel (ADP-induced platelet aggregation at day 6, 103±8% of baseline), ADP-induced aggregation was reduced to 69±3%, 58±7%, and 33±12% of baseline, respectively, in patients in quartiles 2 through 4 (P<0.01 for all). In addition, epinephrine-induced platelet aggregation and platelet aggregation under flow conditions, assessed by the cone-and-plate(let) analyzer method, were reduced significantly less in the first quartile than in quartiles 2 through 4. Whereas 40% of patients in the first quartile sustained a recurrent cardiovascular event during a 6-month follow-up, only 1 patient (6.7%) in the second quartile and none in the third and fourth quartiles suffered a cardiovascular event (P=0.007).
Conclusions Up to 25% of STEMI patients undergoing primary PCI with stenting are resistant to clopidogrel and therefore may be at increased risk for recurrent cardiovascular events.
Key Words: platelets myocardial infarction clopidogrel
| Introduction |
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See p 3064
The antiplatelet effect of clopidogrel has been studied in normal volunteers3 and in patients with stable coronary disease46 but not in patients with ACS, which is associated with platelet activation.7 In the present study, the antiplatelet effect of clopidogrel was studied prospectively in 60 consecutive patients with ST-segmentelevation acute myocardial infarction (STEMI) to determine whether there is significant individual variability in platelet response to clopidogrel and whether it might account for recurrent cardiovascular events.
| Methods |
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Blood samples for testing platelet activity were drawn in the catheterization laboratory before administration of eptifibatide at an average of 80±10 minutes after administration of chewable aspirin (baseline) and daily for 5 days thereafter.
Platelet Function Tests
Turbidimetric Platelet Aggregation
Platelet-rich plasma was prepared by centrifugation of citrated blood, and response to ADP (5 µmol/L) and epinephrine (10 µmol/L) was recorded by use of routine aggregometer (Helena Laboratory; PACKS-4).
Cone-and-Platelet Analyzer
This technology has been described elsewhere.8 Briefly, 200 µL of citrated blood was placed in a polystyrene well and subjected to a shear rate of 1800 s1 using a rotating conical disk for 2 minutes. The well was washed and stained by May-Gruenwald stain. Platelet adhesion was evaluated as the percentage of total area covered with platelets designated as surface coverage (%) and aggregation as the mean size of the surface-bound aggregates designated as average size (µm2) by use of an image analysis system (Galai).
Clinical Follow-Up
All patients were followed up via outpatient clinic visits 3 and 6 months after hospital discharge. Drug therapy compliance was assessed by telephone calls 1 month after discharge and at the first outpatient clinic visit. All medical documents were reviewed in patients who sustained recurrent cardiovascular events or underwent coronary angiography. The treating physician and the investigators who evaluated the clinical end points were blinded to the results of the platelet function activity.
Statistical Analysis
Platelet activity was expressed as a percentage of baseline value. Each patient served as his or her own control, and changes in platelet activity were evaluated with paired t test.
Patients were stratified into 4 quartiles based on the percentage reduction of ADP-induced platelet aggregation at day 6 compared with the baseline ADP-induced platelet aggregation (determined before clopidogrel loading). Categorical variables were presented as percentages in the 4 quartiles and tested for linear trend with Mantel-Haenszel
2 analysis. A 2-tailed Fishers exact test was used to compare patients in the first quartile with patients in the second through fourth quartiles. Continuous variables were presented as mean±SD. The linear trends of the variables in the 4 quartiles were compared by use of Pearson correlation. The Wilcoxon rank-sum test was used to compare patients in the first versus the second through fourth quartiles.
| Results |
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Antiplatelet Effect of Clopidogrel
At day 2 (18±2 hours after clopidogrel loading and 4±0.5 hours after termination of eptifibatide), both mean ADP-induced platelet aggregation and mean epinephrine-induced platelet aggregation were inhibited to 40% of baseline (P<0.01) and at day 3 to 66% (P<0.01) and 78% (P<0.01) of baseline, respectively, with no further significant changes at days 4 through 6 (Figure 1a). The final ADP aggregation (day 6) correlated negatively with the percentage inhibition of baseline ADP-induced platelet aggregation (r2=0.94, P<0.01).
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The aggregate size, assessed by cone-and-platelet analysis, which reflects platelet aggregation under flow conditions, was significantly decreased to 60% of baseline value at day 2 and to
70% at day 6 (Figure 1b). Although the surface coverage was decreased significantly, by
50% (P<0.01), at day 2 when an eptifibatide effect could still be expected, clopidogrel had no significant effect on surface coverage on day 3 and thereafter (
90% of baseline at days 3 through 6, P=0.16).
In patients who underwent angioplasty without stenting and therefore were treated with aspirin alone, no significant inhibition of ADP- and epinephrine-induced platelet aggregation at days 3 through 6 was noted compared with baseline activity, which had already been determined after aspirin loading. At each of these time points, platelet reactivity was significantly higher than in patients treated with both aspirin and clopidogrel (P<0.01, Figure 1c).
Variability in Platelet Response to Clopidogrel
When patients were stratified into 4 quartiles according to percentage reduction of ADP-induced platelet aggregation at day 6 compared with the baseline activity of each patient, significant variability was noted. Whereas patients in the first quartile were resistant to the effect of clopidogrel (ADP-induced platelet aggregation at day 6, 103±8% of baseline), ADP-induced platelet aggregation was reduced significantly in patients in the second through fourth quartiles, to 69±3%, 58±7%, and 33±12% of the respective baselines (P<0.01 for all, Figure 2a). Inhibition in platelet aggregation increased significantly in the first through fourth quartiles (P for trend <0.01). Patients in the first through fourth quartiles also exhibited increasing reduction in epinephrine-induced platelet aggregation (P for trend <0.001) and in aggregate size, reflecting aggregation under flow conditions (Figure 2b).
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Clinical Outcomes
There was a gradual and significant increase in the number of cigarette smokers in the first through fourth quartiles (Table), but otherwise there were no significant differences in the other baseline demographic and clinical characteristics, platelet counts on admission and at day 6, indices of infarct size, and angiographic findings (Table). Similarly, there were no significant differences in the medications assigned to patients during hospitalization and at hospital discharge in all 4 quartiles (Table). Postinterventional heparin was discontinued in all patients, and none of the patients received anticoagulant therapy throughout the follow-up period.
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None of the 60 patients enrolled in the study were lost to the 6-month follow-up. During this period, 2 patients developed recurrent STEMI, one of whom had 2 recurrent MIs because of stent thrombosis; 3 had recurrent ACS; and a sixth suffered an acute peripheral arterial occlusion, necessitating urgent surgery. One patient died of ischemic stroke. Thus, 7 patients had 8 recurrent cardiovascular events, 6 of which occurred during treatment with clopidogrel. Seven of the 8 events (88%) occurred in patients resistant to clopidogrel (first quartile) and 1 event (12%) in a patient from the second quartile. Accordingly, whereas 40% of patients in the first quartile (clopidogrel resistant) sustained a recurrent cardiovascular event, only 1 patient (6.7%) in the second quartile and none in the third and fourth quartiles developed an event (P for trend=0.007, Figure 2c). In contrast, major bleeding occurred in 2 patients, both of whom were in the fourth quartile.
Patients with recurrent cardiovascular events were older (P=0.008), had higher Killip class on admission (P=0.004), and had a lower percentage reduction of ADP-induced platelet aggregation as early as day 3 (91±21% versus 62±21% percent of baseline, P<0.001), which persisted through day 6 (90±16% versus 64±27%, P<0.001, Figure 3).
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| Discussion |
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Clopidogrel Effects in AMI Patients
Clopidogrel yielded a 35% to 60% reduction in ex vivo platelet aggregation in normal volunteers and in patients with stable coronary disease.36 Clopidogrel did not confer significant inhibition of platelets, which are activated by the thrombin-related activating peptide (TRAP), suggesting that clopidogrel might be less effective in clinical situations characterized by high thrombin generation, as in ACS.5,6 Furthermore, previous studies have shown that there is a marked increase in platelet activity in patients with AMI undergoing PCI7 and that pretreatment platelet activity may negatively affect the antithrombotic effect of clopidogrel.9 These findings warrant investigation of the antiplatelet effect of clopidogrel in patients with AMI. In the present study, clopidogrel administration in STEMI patients undergoing primary PCI who were already receiving aspirin was followed by a significant inhibition of ADP-induced platelet aggregation of 35% and a significant reduction of 25% in aggregate size, indicating inhibition of aggregation under flow conditions. Clopidogrel also significantly inhibited epinephrine-induced platelet aggregation, probably by blocking the effect of secondarily released ADP.
Clopidogrel Resistance
Recently, laboratory documentation of aspirin resistance has been shown to predict an increased risk of cardiovascular events in patients with coronary artery disease.12 A few studies have revealed important individual heterogeneity in platelet response to clopidogrel in patients with stable coronary disease,911 but the clinical significance of this phenomenon has not yet been investigated. We expanded this finding by showing similar marked individual variability in response to clopidogrel in patients with STEMI, demonstrating that such heterogenicity may possess important clinical implications. In the present study, as many as 25% of patients with STEMI were resistant to clopidogrel and subsequently were at increased risk of recurrent cardiovascular events in a 6-month follow-up.
In the present study, smoking seemed to enhance the clopidogrel antiplatelet effect with a consistent gradual increase of the number of cigarette smokers from the first to the fourth quartile, opposing the possibility of this being a chance finding. Aside from smoking, no other baseline characteristic predicted the response to clopidogrel.
Clopidogrel is a prodrug, and the active metabolite formation is regulated primarily by cytochrome P450 isoenzymes 3A4 and 1A2, the latter of which is activated by the polycyclic aromatic hydrocarbons that exist in cigarette smoke.13 Furthermore, plasma nicotine levels similar to those reported in human smokers were shown to induce cytochrome 1A2 in rats.14 This possible explanation is in agreement with the findings of Lau et al,15 who showed that in normal volunteers and patients after coronary stenting, the individual platelet variability in response to clopidogrel correlates with cytochrome P450 metabolism activity. However, polymorphism in the ADP receptor or differences in the postreceptor signaling pathway cannot be excluded as an additional explanation for the variability in platelet responsiveness and clopidogrel resistance.
It should be mentioned that the present study is an observational one, comprising a relatively small sample size, and therefore does not allow for definitive conclusions. Nevertheless, clopidogrel resistance occurs in a significant percentage of STEMI patients and is associated with a higher risk of recurrent cardiovascular events. The question of whether increased doses of clopidogrel might overcome this resistance in nonresponsive patients warrants further investigation.
| Acknowledgments |
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| Footnotes |
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| References |
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L. Bonello, L. Camoin-Jau, S. Arques, C. Boyer, D. Panagides, O. Wittenberg, M.-C. Simeoni, P. Barragan, F. Dignat-George, and F. Paganelli Adjusted Clopidogrel Loading Doses According to Vasodilator-Stimulated Phosphoprotein Phosphorylation Index Decrease Rate of Major Adverse Cardiovascular Events in Patients With Clopidogrel Resistance: A Multicenter Randomized Prospective Study J. Am. Coll. Cardiol., April 8, 2008; 51(14): 1404 - 1411. [Abstract] [Full Text] [PDF] |
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M. J. Price, S. Endemann, R. R. Gollapudi, R. Valencia, C. T. Stinis, J. P. Levisay, A. Ernst, N. S. Sawhney, R. A. Schatz, and P. S. Teirstein Prognostic significance of post-clopidogrel platelet reactivity assessed by a point-of-care assay on thrombotic events after drug-eluting stent implantation Eur. Heart J., April 2, 2008; 29(8): 992 - 1000. [Abstract] [Full Text] [PDF] |
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D. R. Holmes Jr Resistance to what, does it matter? How do we study it? Eur. Heart J., April 2, 2008; 29(8): 957 - 958. [Full Text] [PDF] |
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S. Muller-Schunk, J. Linn, N. Peters, M. Spannagl, M. Deisenberg, H. Bruckmann, and T.E. Mayer Monitoring of Clopidogrel-Related Platelet Inhibition: Correlation of Nonresponse with Clinical Outcome in Supra-Aortic Stenting AJNR Am. J. Neuroradiol., April 1, 2008; 29(4): 786 - 791. [Abstract] [Full Text] [PDF] |
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D. J. Kereiakes and P. A. Gurbel Peri-Procedural Platelet Function and Platelet Inhibition in Percutaneous Coronary Intervention J. Am. Coll. Cardiol. Intv., April 1, 2008; 1(2): 111 - 121. [Abstract] [Full Text] [PDF] |
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M Srinivasan and D Smith Drug interaction with anti-mycobacterial treatment as a cause of clopidogrel resistance Postgrad. Med. J., April 1, 2008; 84(990): 217 - 219. [Abstract] [Full Text] [PDF] |
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P. L. L'Allier, G. Ducrocq, N. Pranno, S. Noble, R. Ibrahim, J. C. Gregoire, F. Azzari, A. Nozza, C. Berry, S. Doucet, et al. Clopidogrel 600-mg double loading dose achieves stronger platelet inhibition than conventional regimens: results from the PREPAIR randomized study. J. Am. Coll. Cardiol., March 18, 2008; 51(11): 1066 - 1072. [Abstract] [Full Text] [PDF] |
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A. D. Michelson P2Y12 Antagonism: Promises and Challenges Arterioscler Thromb Vasc Biol, March 1, 2008; 28(3): s33 - s38. [Abstract] [Full Text] [PDF] |
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S. Prabhakaran, K.R Wells, V.H. Lee, C.A. Flaherty, and D.K. Lopes Prevalence and Risk Factors for Aspirin and Clopidogrel Resistance in Cerebrovascular Stenting AJNR Am. J. Neuroradiol., February 1, 2008; 29(2): 281 - 285. [Abstract] [Full Text] [PDF] |
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M. Gilard, B. Arnaud, J.-C. Cornily, G. Le Gal, K. Lacut, G. Le Calvez, J. Mansourati, D. Mottier, J.-F. Abgrall, and J. Boschat Influence of Omeprazole on the Antiplatelet Action of Clopidogrel Associated With Aspirin: The Randomized, Double-Blind OCLA (Omeprazole CLopidogrel Aspirin) Study J. Am. Coll. Cardiol., January 22, 2008; 51(3): 256 - 260. [Abstract] [Full Text] [PDF] |
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L. Wallentin, C. Varenhorst, S. James, D. Erlinge, O. O Braun, J. A. Jakubowski, A. Sugidachi, K. J. Winters, and A. Siegbahn Prasugrel achieves greater and faster P2Y12receptor-mediated platelet inhibition than clopidogrel due to more efficient generation of its active metabolite in aspirin-treated patients with coronary artery disease Eur. Heart J., January 1, 2008; 29(1): 21 - 30. [Abstract] [Full Text] [PDF] |
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R. F. Storey Variability of response to antiplatelet therapy Eur. Heart J. Suppl., January 1, 2008; 10(suppl_A): A21 - A27. [Abstract] [Full Text] [PDF] |
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C. Gachet and B. Aleil Testing antiplatelet therapy Eur. Heart J. Suppl., January 1, 2008; 10(suppl_A): A28 - A34. [Abstract] [Full Text] [PDF] |
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S. D. Wiviott, D. Trenk, A. L. Frelinger, M. O'Donoghue, F.-J. Neumann, A. D. Michelson, D. J. Angiolillo, H. Hod, G. Montalescot, D. L. Miller, et al. Prasugrel Compared With High Loading- and Maintenance-Dose Clopidogrel in Patients With Planned Percutaneous Coronary Intervention: The Prasugrel in Comparison to Clopidogrel for Inhibition of Platelet Activation and Aggregation Thrombolysis in Myocardial Infarction 44 Trial Circulation, December 18, 2007; 116(25): 2923 - 2932. [Abstract] [Full Text] [PDF] |
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S. D. Wiviott, E. Braunwald, C. H. McCabe, G. Montalescot, W. Ruzyllo, S. Gottlieb, F.-J. Neumann, D. Ardissino, S. De Servi, S. A. Murphy, et al. Prasugrel versus Clopidogrel in Patients with Acute Coronary Syndromes N. Engl. J. Med., November 15, 2007; 357(20): 2001 - 2015. [Abstract] [Full Text] [PDF] |
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P. A. Gurbel, R. C. Becker, K. G. Mann, S. R. Steinhubl, and A. D. Michelson Platelet Function Monitoring in Patients With Coronary Artery Disease J. Am. Coll. Cardiol., November 6, 2007; 50(19): 1822 - 1834. [Abstract] [Full Text] [PDF] |
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C. P. Cannon, S. Husted, R. A. Harrington, B. M. Scirica, H. Emanuelsson, G. Peters, R. F. Storey, and for the DISPERSE-2 Investigators Safety, Tolerability, and Initial Efficacy of AZD6140, the First Reversible Oral Adenosine Diphosphate Receptor Antagonist, Compared With Clopidogrel, in Patients With Non-ST-Segment Elevation Acute Coronary Syndrome: Primary Results of the DISPERSE-2 Trial J. Am. Coll. Cardiol., November 6, 2007; 50(19): 1844 - 1851. [Abstract] [Full Text] [PDF] |
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R. F. Storey, S. Husted, R. A. Harrington, S. Heptinstall, R. G. Wilcox, G. Peters, M. Wickens, H. Emanuelsson, P. Gurbel, P. Grande, et al. Inhibition of Platelet Aggregation by AZD6140, A Reversible Oral P2Y12 Receptor Antagonist, Compared With Clopidogrel in Patients With Acute Coronary Syndromes J. Am. Coll. Cardiol., November 6, 2007; 50(19): 1852 - 1856. [Abstract] [Full Text] [PDF] |
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D. I. Simon and A. H. Schmaier Sweet and Sticky: Diabetic Platelets, Enhanced Reactivity, and Cardiovascular Risk J. Am. Coll. Cardiol., October 16, 2007; 50(16): 1548 - 1550. [Full Text] [PDF] |
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D. J. Angiolillo, E. Bernardo, M. Sabate, P. Jimenez-Quevedo, M. A. Costa, J. Palazuelos, R. Hernandez-Antolin, R. Moreno, J. Escaned, F. Alfonso, et al. Impact of Platelet Reactivity on Cardiovascular Outcomes in Patients With Type 2 Diabetes Mellitus and Coronary Artery Disease J. Am. Coll. Cardiol., October 16, 2007; 50(16): 1541 - 1547. [Abstract] [Full Text] [PDF] |
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G. Campo, M. Valgimigli, D. Gemmati, G. Percoco, L. Catozzi, A. Frangione, F. Federici, F. Ferrari, M. Tebaldi, S. Luccarelli, et al. Poor Responsiveness to Clopidogrel: Drug-Specific or Class-Effect Mechanism?: Evidence From a Clopidogrel-to-Ticlopidine Crossover Study J. Am. Coll. Cardiol., September 18, 2007; 50(12): 1132 - 1137. [Abstract] [Full Text] [PDF] |
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G. J. Weerakkody, J. A. Jakubowski, J. T. Brandt, C. D. Payne, H. Naganuma, and K. J. Winters Greater Inhibition of Platelet Aggregation and Reduced Response Variability With Prasugrel Versus Clopidogrel: An Integrated Analysis Journal of Cardiovascular Pharmacology and Therapeutics, September 1, 2007; 12(3): 205 - 212. [Abstract] [PDF] |
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J. L. Anderson, C. D. Adams, E. M. Antman, C. R. Bridges, R. M. Califf, D. E. Casey Jr, W. E. Chavey II, F. M. Fesmire, J. S. Hochman, T. N. Levin, et al. ACC/AHA 2007 Guidelines for the Management of Patients With Unstable Angina/Non-ST-Elevation Myocardial Infarction: A Report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Writing Committee to Revise the 2002 Guidelines for the Management of Patients With Unstable Angina/Non-ST-Elevation Myocardial Infarction) Developed in Collaboration with the American College of Emergency Physicians, the Society for Cardiovascular Angiography and Interventions, and the Society of Thoracic Surgeons Endorsed by the American Association of Cardiovascular and Pulmonary Rehabilitation and the Society for Academic Emergency Medicine J. Am. Coll. Cardiol., August 14, 2007; 50(7): e1 - e157. [Full Text] [PDF] |
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J. L. Anderson, C. D. Adams, E. M. Antman, C. R. Bridges, R. M. Califf, D. E. Casey Jr, W. E. Chavey II, F. M. Fesmire, J. S. Hochman, T. N. Levin, et al. ACC/AHA 2007 Guidelines for the Management of Patients With Unstable Angina/Non ST-Elevation Myocardial Infarction Executive Summary: A Report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Writing Committee to Revise the 2002 Guidelines for the Management of Patients With Unstable Angina/Non ST-Elevation Myocardial Infarction) Developed in Collaboration with the American College of Emergency Physicians, the Society for Cardiovascular Angiography and Interventions, and the Society of Thoracic Surgeons Endorsed by the American Association of Cardiovascular and Pulmonary Rehabilitation and the Society for Academic Emergency Medicine J. Am. Coll. Cardiol., August 14, 2007; 50(7): 652 - 726. [Full Text] [PDF] |
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N. von Beckerath, A. Kastrati, A. Wieczorek, G. Pogatsa-Murray, D. Sibbing, I. Graf, and A. Schomig A double-blind, randomized study on platelet aggregation in patients treated with a daily dose of 150 or 75 mg of clopidogrel for 30 days Eur. Heart J., August 1, 2007; 28(15): 1814 - 1819. [Abstract] [Full Text] [PDF] |
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C. Bode and M. Zehender The use of antiplatelet agents following percutaneous coronary intervention: focus on late stent thrombosis Eur. Heart J. Suppl., August 1, 2007; 9(suppl_D): D10 - D19. [Abstract] [Full Text] [PDF] |
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D. R. Holmes Jr, D. J. Kereiakes, W. K. Laskey, A. Colombo, S. G. Ellis, T. D. Henry, J. J. Popma, P. W.J.C. Serruys, T. Kimura, D. O. Williams, et al. Thrombosis and Drug-Eluting Stents: An Objective Appraisal J. Am. Coll. Cardiol., July 10, 2007; 50(2): 109 - 118. [Abstract] [Full Text] [PDF] |
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Authors/Task Force Members, J.-P. Bassand, C. W. Hamm, D. Ardissino, E. Boersma, A. Budaj, F. Fernandez-Aviles, K. A.A. Fox, D. Hasdai, E. M. Ohman, et al. Guidelines for the diagnosis and treatment of non-ST-segment elevation acute coronary syndromes: The Task Force for the Diagnosis and Treatment of Non-ST-Segment Elevation Acute Coronary Syndromes of the European Society of Cardiology Eur. Heart J., July 1, 2007; 28(13): 1598 - 1660. [Full Text] [PDF] |
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A. El-Khuffash and E. J Molloy Are B-type natriuretic peptide (BNP) and N-terminal-pro-BNP useful in neonates? Arch. Dis. Child. Fetal Neonatal Ed., July 1, 2007; 92(4): F320 - F324. [Abstract] [Full Text] [PDF] |
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D. J. Moliterno and C. L. Campbell Unraveling Questions Surrounding Clopidogrel Resistance and Stent Thrombosis: One Less Snag J. Am. Coll. Cardiol., June 19, 2007; 49(24): 2318 - 2319. [Full Text] [PDF] |
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P. Buonamici, R. Marcucci, A. Migliorini, G. F. Gensini, A. Santini, R. Paniccia, G. Moschi, A. M. Gori, R. Abbate, and D. Antoniucci Impact of Platelet Reactivity After Clopidogrel Administration on Drug-Eluting Stent Thrombosis J. Am. Coll. Cardiol., June 19, 2007; 49(24): 2312 - 2317. [Abstract] [Full Text] [PDF] |
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T. A. Meadows and D. L. Bhatt Clinical Aspects of Platelet Inhibitors and Thrombus Formation Circ. Res., May 11, 2007; 100(9): 1261 - 1275. [Abstract] [Full Text] [PDF] |
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A. O. Maree and D. J. Fitzgerald Variable Platelet Response to Aspirin and Clopidogrel in Atherothrombotic Disease Circulation, April 24, 2007; 115(16): 2196 - 2207. [Full Text] [PDF] |
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D. J. Angiolillo, A. Fernandez-Ortiz, E. Bernardo, F. Alfonso, C. Macaya, T. A. Bass, and M. A. Costa Variability in Individual Responsiveness to Clopidogrel: Clinical Implications, Management, and Future Perspectives J. Am. Coll. Cardiol., April 10, 2007; 49(14): 1505 - 1516. [Abstract] [Full Text] [PDF] |
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