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(Circulation. 2000;101:1199.)
© 2000 American Heart Association, Inc.

Basic Science Reports

Losartan and Its Metabolite E3174 Modify Cardiac Delayed Rectifier K⁺ Currents

Ricardo Caballero, BPharm; Eva Delpón, BPharm, PhD; Carmen Valenzuela, BSc, PhD; Mónica Longobardo, BPharm; Juan Tamargo, MD, PhD, FESC

From the Department of Pharmacology, School of Medicine, Universidad Complutense, Madrid, Spain.

Correspondence to Eva Delpón, Department of Pharmacology, School of Medicine, Universidad Complutense, 28040 Madrid, Spain.

	Abstract

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Background—The effects of type 1 angiotensin II receptor antagonist losartan and its metabolite E3174 on transmembrane action potentials, hKv1.5, HERG, and I_Ks currents were analyzed.

Methods and Results—Guinea pig ventricular action potentials were recorded with microelectrode techniques and hKv1.5 and HERG currents with the whole-cell patch-clamp technique. I_Ks was recorded in guinea pig ventricular myocytes with the perforated-nystatin-patch configuration. Losartan and E3174 transiently increased the hKv1.5 current by 8.0±1.4% and 7.4±1.6%, respectively. Thereafter, they produced a voltage-dependent block, E3174 being more potent than losartan (P<0.05) for this effect. Losartan decreased HERG currents elicited at 0 mV (23.3±4.8%), whereas E3174 increased the current (30.5±6.2%). Both drugs shifted the midpoint of the activation curve of HERG channels to more negative potentials. In ventricular myocytes, losartan and E3174 inhibited the I_Ks (18.4±3.2% and 6.5±0.7%, respectively). Losartan-induced block was voltage-independent, whereas E3174 shifted the midpoint of the activation curve to more negative potentials. Losartan lengthened the duration of the action potentials at both 50% and 90% of repolarization, whereas E3174 slowed only the final phase of the repolarization process.

Conclusions—These results demonstrated that at therapeutic concentrations, both losartan and E3174 modified the cardiac delayed rectifier hKv1.5, HERG, and Ks currents.

Key Words: losartan • E3174 • ion channels • myocytes • electrophysiology

	Introduction

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The renin-angiotensin system may contribute to cardiac arrhythmias in various cardiovascular diseases, including congestive heart failure and ischemic heart disease.¹ Angiotensin II exerts its effects by binding to specific receptors in the plasma membrane of effector cells.¹ Losartan, a competitive angiotensin II type 1 (AT₁) receptor antagonist, is an effective antihypertensive agent.² In the ELITE study,³ the apparent mortality advantage for the losartan group seems to be primarily due to a reduction in sudden death, which suggests a potential antiarrhythmic effect of the drug. In cardiac Purkinje fibers, losartan had no effect on transmembrane action potentials,⁴ but in in vitro models of ischemia and reperfusion, it exhibited antiarrhythmic activity that could not be attributed to AT₁ receptor blockade, because this occurred in the absence of angiotensin II.^{5 6} Losartan is rapidly metabolized to E-3174, a noncompetitive AT₁ receptor antagonist more potent and with a longer half-life than the parent drug.² Thus, E3174 may be responsible for some effects attributed to losartan.

The precise mechanisms of the potential antiarrhythmic effects of losartan are unclear. Losartan had no effect on Na⁺ and Ca²⁺ currents in canine Purkinje fibers⁴ and ventricular myocytes,⁷ but its effects on cardiac K⁺ channels are unknown. Therefore, the present study was undertaken to study the direct effects of losartan and E-3174 on hKv1.5 and HERG channels cloned from human heart and on the delayed rectifier K⁺ (I_K) current recorded in guinea pig ventricular myocytes.

	Methods

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Transmembrane Action Potentials
Transmembrane action potentials were recorded in guinea pig (250 to 300 g) papillary muscles driven at 1 Hz through conventional microelectrode techniques as previously described.⁸ Experiments were performed at 34°C.

Isolation of Single Guinea Pig Ventricular Myocytes and Cell Culture
Single ventricular myocytes were isolated by use of collagenase and protease digestion as described previously.⁹ Stably transfected Ltk^- cells were cultured in DMEM with 10% horse serum and 0.25 mg/mL G418 (Gibco) in a 5% CO₂ atmosphere as previously described.^{10 11} Chinese hamster ovary (CHO) cultures were grown in Hams-F12 medium with 10% FBS and transiently transfected with the cDNA encoding the HERG channel (4 µg/mL) and cDNA encoding the CD8 antigen (0.5 µg/mL) by use of lipofectamine. Before experimental use, cells were incubated with polystyrene microbeads precoated with anti-CD8 antibody (Dynabeads M450, Dynal). Most of the cells that were beaded also had channel expression.

Solutions
To measure I_K in guinea pig ventricular myocytes, the external solution contained (mmol/L) NaCl 136, KCl 5.4, CaCl₂ 1.0, MgCl₂ 1.0, CoCl₂ 2.0, tetrodotoxin 0.03, glucose 10, and HEPES 10 (pH adjusted to 7.4 with NaOH). Under these conditions, sodium and calcium currents were blocked by tetrodotoxin and CoCl₂, respectively. To analyze the effects on I_Ks, the solution was supplemented with 30 µmol/L LaCl₃ to block Kr channels.⁹ Papillary muscles were perfused with the Co²⁺- or the Co²⁺+La³⁺-containing solution, in which tetrodotoxin was omitted. Ltk^- and CHO cells were perfused with an external solution containing (mmol/L) NaCl 130, KCl 4, CaCl₂ 1, MgCl₂ 1, HEPES 10, and glucose 10 (pH adjusted to 7.4 with NaOH). The internal solution contained (mmol/L) potassium aspartate 80, KCl 42, KH₂PO₄ 10, MgATP 5, phosphocreatine 3, HEPES 5, and EGTA 5 (pH adjusted to 7.2 with KOH). Losartan and E3174 (Merck Sharp & Dohme España) were dissolved in methanol to make 1 mmol/L stock solution.

Recording Techniques
hKv1.5 and HERG currents were measured with the whole-cell patch-clamp technique. In ventricular myocytes, I_K was recorded with the perforated-nystatin-patch configuration to avoid the rundown of the current.⁹ Recordings were performed at 24°C to 25°C with 200B patch-clamp amplifiers and pClamp 6.1 software (Axon Instruments). Pipettes had a tip resistance <3 M when filled with the internal solution. Cell capacitance and access resistance were calculated for each cell. Thereafter, capacitance and series resistance compensation were optimized, and 80% compensation was usually obtained. Maximum hKv1.5 current amplitudes at +60 mV averaged 1.5±0.1 nA, mean uncompensated access resistance was 3.2±0.5 M, and cell capacitance was 10.2±0.9 pF (n=22). Thus, no significant voltage errors (<5 mV) were expected with the electrodes used. In ventricular myocytes, the effective access resistance calculated was 13.4±0.8 M (n=8), and the larger currents recorded were <1 nA (278±33 pA); thus, the mean value of voltage error has an upper limit of 2.7 mV. The current records were sampled at 3 to 10 times the antialias filter setting.

The activation curves were constructed by plotting tail current amplitudes elicited as a function of the membrane potential and were fitted with a Boltzmann distribution:

(1)

where A is the amplitude, V_h is the midpoint of activation, V_m is the test potential, and k represents the slope factor. Under some circumstances, a Boltzmann distribution with 2 terms was needed to fit the experimental data, the equation being

(2)

To determine the voltage dependence of hKv1.5 current block, the leak-corrected current in the presence of drug was normalized to matching control to yield the fractional block at each voltage. The voltage dependence of block was fitted to

(3)

where z, F, R, and T have their usual meaning, represents the fractional electrical distance, and K_D* represents the apparent dissociation constant at the reference potential (0 mV).

Time-dependent drug effects on I_Ks were quantified by measurement of the amplitudes of tail currents elicited by depolarizing pulses from -40 to +50 mV of variable duration (0.25 to 10 seconds). The relation between the amplitude tails and the pulse duration was fitted by a monoexponential time function. To describe the time course of the tail currents on repolarization, exponential analysis was used.

Results are expressed as mean±SEM. Data were compared by ANOVA followed by Newman-Keuls test. A value of P<0.05 was considered significant.

	Results

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Effects on hKv1.5 Currents
Figure 1 shows hKv1.5 currents recorded in 2 cells in the absence of and 4 minutes after perfusion of 1 µmol/L losartan (A) or E3174 (B). At the beginning of the infusion, losartan and E3174 increased the amplitude of hKv1.5 currents elicited by 250-ms pulses from -80 to +60 mV by 8.0±1.4% (n=8) and 7.4±1.6% (n=8), respectively. Thereafter, the current amplitude slowly declined until a new steady-state level was achieved (within 10 minutes). Figure 1, C and D, shows current traces elicited by 500-ms pulses from -80 mV at 0.1 Hz. E3174 decreased the current amplitude at the end of the pulses to +60 mV more than losartan (37.2±4.8% versus 8.7±2.3%, P<0.05). Figure 1, E and F, shows tail currents elicited on return to -40 mV. Losartan increased the time constant of tail current decline from 63.5±9.6 to 117.7±16.8 ms (n=8, P<0.01). E3174 markedly decreased the peak tail amplitude and increased the time constant of tail current decline from 56.4±6.4 to 111.2±13.4 ms (n=16, P<0.01), inducing the "crossover" of the tail currents.

View larger version (18K): [in this window] [in a new window]   Figure 1. A and B, Effects of losartan and E3174, 1 µmol/L, on hKv1.5 currents recorded in 2 different cells 4 minutes after beginning of infusion of drug. Currents were elicited by 250-ms pulses to +60 mV from a holding of -80 mV. C and D, Steady-state effects of losartan and E3174. Superimposed current traces are shown for 500-ms pulses from -80 mV to voltages between +60 and -80 mV in 20-mV steps. E and F, Effects of losartan and E3174 on tail currents elicited on return to -40 mV after pulses to +60 mV. The arrow indicates tail current crossover. Dotted line represents zero current level.

Voltage- and Frequency-Dependent Effects of Losartan and E3174 on hKv1.5 Currents
Figure 2A shows the current-voltage relationship (I-V curves) obtained in the absence and in the presence of losartan (left) or E3174 (right). At negative potentials (-30/-20 mV), both drugs increased the current amplitude, whereas at depolarized potentials, an inhibitory effect was observed. To quantify this voltage dependence, the ratio I_drug/I_control for each experiment was plotted as a function of the membrane potential, together with the activation curve obtained in control conditions (Figure 2B). For both drugs, the blockade reached a maximum at 0 mV and thereafter decreased with a shallow voltage-dependence. Figure 2B shows that the blockade induced by losartan and E3174 was significantly higher at +10 than at +60 mV (P<0.05). Fitting the experimental data to Equation 3, the z value averaged -0.27±0.04 and -0.14±0.01 in the presence of losartan and E3174, respectively. Moreover, losartan and E3174 dramatically modified the voltage-dependence of channel activation. Under control conditions, the averaged values for V_h and k of the activation curve were -19.5±1.2 and 4.9±0.3 mV (n=16), respectively (Figure 2C). In the presence of losartan or E3174, the activation curve displayed 2 components, the steeper one being responsible for 75% of the activation process. The solid lines illustrate a fit with a sum of 2 Boltzmann components, and the V_h and k values for each component were summarized in Table 1. Both losartan and E3174 significantly shifted the midpoint of the steeper component to more negative potentials.

View larger version (33K): [in this window] [in a new window]   Figure 2. A, I-V relationship (500 ms isochronal) of hKv1.5 channels in absence and presence of 1 µmol/L losartan (left) or E3174 (right). *P<0.05 vs control. B, Fractional block (Idrug/Icontrol) from data shown in A. Dotted lines represent activation curve for each group of experiments. Continuous lines represent best fit to data positive to 0 mV (see Equation 3). *P<0.05 vs values at +60 mV. C, Effects of losartan (left) and E3174 (right) on voltage-dependence of hKv1.5 channel activation. Activation curves in absence of drug were fitted with a single Boltzmann component (dotted line; Equation 1). Dashed lines show that this approach was not optimal for data in presence of losartan or E3174, whereas better fits were obtained with a sum of 2 Boltzmann components (solid line; Equation 2). Each data point represents mean and vertical lines, SEM of 8 experiments.

View this table: [in this window] [in a new window]   Table 1. Effects of Losartan and E3174, 1 µmol/L, on the Voltage-Dependence of hKv1.5 Channel Activation

In another group of experiments, trains of 200-ms pulses to +10 or +60 mV at 1 or 2 Hz were applied. Trains were separated from each other by 2-minute intervals. Under control conditions, the current amplitude decreased by 7.0±0.5% and 16.3±1.2% when trains of pulses to +10 mV were applied at 1 and 2 Hz, respectively. The percentages of block obtained from the ratio of current amplitudes in the absence and in the presence of either losartan or E3174 during application of the trains were averaged (Table 2). A certain amount of block was apparent from the first depolarization applied, ie, "tonic block." Thereafter, during the train, the blockade increased until a new steady-state level was reached, ie, the blockade was frequency-dependent. Moreover, the frequency-dependent block produced by E3174 was more marked than that induced by losartan (Table 2).

View this table: [in this window] [in a new window]   Table 2. Frequency-Dependent Effects Induced by Losartan and E3174

Effects on K⁺ Currents in Ventricular Myocytes
Figure 3, A and B, shows current traces elicited in 2 guinea pig ventricular myocytes perfused with Co²⁺-containing solution when 5-second pulses were applied from -40 to +50 mV. Under these conditions, the outward current is the sum of the fast (I_Kr) and the slow (I_Ks) components of the delayed rectifier.⁹ At 1 µmol/L, losartan decreased the maximum outward current at the end of the pulse to +50 mV by 27.5±1.8%, (P<0.01, n=6). Steady-state block was voltage-independent; thus, blockade after pulses to -10 mV averaged 22.1±2.4% (n=6, P>0.05). Figure 3B shows that at 1 µmol/L, E3174 transiently increased the total maximum outward current elicited on depolarization by 9.8±1.8% (P<0.05), but after 10 to 15 minutes of perfusion, the current amplitude decreased by 8.2±1.7% (P<0.05, n=6). At 10 µmol/L, E3174 decreased the maximum outward current by 16.0±1.5% (P<0.05) without any significant modification of the tail current. Figure 3C shows the current traces elicited in the same cell as in 3B when the steady-state effects were achieved. After a 5-second depolarization to +50 mV, the cell was repolarized at 0 mV for 10 seconds. Under these conditions, the I_Ks was deactivated, and hyperpolarization to -50 mV elicited a tail current that is mainly due to the I_Kr because of its marked inward rectifier properties. E3174 at 1 µmol/L slightly decreased the maximum and the tail currents elicited by the first depolarizing pulse, but increased (Figure 3D) the tail amplitude obtained at -50 mV by 15.3±2.3% (P<0.05, n=3).

View larger version (17K): [in this window] [in a new window]   Figure 3. A, Effects of 1 µmol/L losartan on IK elicited by a 5-second pulse from -80 mV to +50 mV and on tail currents elicited on repolarization to -30 mV. B, Effects of 1 and 10 µmol/L E3174 on IK. C, Current records obtained by applying pulse protocol shown at top in same cell as in B when effects of 1 µmol/L E3174 reached steady state. D, Expanded scale of tail currents elicited by latter hyperpolarization to -50 mV. A through C, Dotted line represents zero current level.

Effects on HERG Channels
Figure 4 shows the effects of losartan and E3174 on HERG currents. Depolarizations to potentials positive to -50 mV elicited an increasing outward current that was followed by a pronounced deactivating tail current on repolarization to -60 mV (Figure 4, A and B). The I-V curves were obtained by plotting the HERG current amplitude at the end of 5-second pulses as a function of the membrane potential in control conditions and in the presence of 1 µmol/L losartan (C) and E3174 (D). The current amplitude reached a maximum at 0 mV, decreasing at more positive voltages, consistent with the marked inward rectification observed for I_Kr.¹² Losartan decreased HERG currents elicited at 0 mV by 23.3±4.8% (P<0.05), whereas E3174 increased the current by 30.5±6.2% (from 133±36 to 175±74 pA, P>0.05). Both drugs shifted the V_h of the activation curve into the negative direction. In fact, V_h in the absence and in the presence of losartan averaged -12.7±1.5 and -19.9±3.6 mV (n=5, P>0.05), respectively, whereas E3174 shifted the V_h 10.8±2.1 mV in the hyperpolarizing direction (n=5, P<0.05). Deactivating tail currents at -60 mV after pulses to +60 mV exhibited a biexponential time course with a fast (230.9±30.5 ms) and a slow (1390.4±161.9 ms) time constant. Losartan did not modify the fast (217.2±39.4 ms, P>0.05) or the slow (1285.6±279.6 ms, n=5, P>0.05) time constants of deactivation. E3174 also failed to modify the time course of the deactivating tails (238.6±38.9 and 1417.4±213.6 ms, n=5, P>0.05).

View larger version (26K): [in this window] [in a new window]   Figure 4. Effects of 1 µmol/L losartan (A) and 1 µmol/L E3174 (B) on HERG currents elicited by 5-second pulses from -80 to +20 mV (A) or to +10 mV (B) followed by repolarizations to -60 mV. C and D, I-V relationships (5 seconds isochronal) of HERG channels recorded in absence and in presence of losartan (C) or E3174 (D). Each point represents mean±SEM of 5 cells. *P<0.05 vs control.

Voltage- and Time-Dependent Effects on I_Ks
Effects of losartan and E3174 on I_Ks were studied in ventricular cells perfused with an external solution supplemented with LaCl₃. Figure 5A shows that 1 µmol/L losartan decreased both the maximum outward and the tail current amplitudes. This blockade was not voltage-dependent, averaging 18.4±3.2% and 22.0±4.2% when pulses to +50 and to -10 mV were applied (n=6, P>0.05). The dotted representation is the ratio between the losartan-sensitive current (I_C-I_L) and the current in control conditions. Fitting this ratio to a monoexponential function yielded the time constant of development of block, which averaged 481.1±64.7 ms (n=6). Furthermore, losartan (Figure 5C) did not modify the V_h of the I_Ks activation curve (26.7±2.5 versus 26.3±2.4 mV, n=6, P>0.05), but it increased the k value from 22.4±1.5 to 19.2±1.6 mV (n=6, P<0.05).

View larger version (23K): [in this window] [in a new window]   Figure 5. Effects of losartan and E3174 on IKs recorded in guinea pig myocytes. A, Effects of 1 µmol/L losartan on IKs recorded by applying 5-second pulse from -40 mV to +50 mV and on repolarization to -30 mV in a myocyte perfused with La3+-containing solution. Dotted representation is plot showing current ratio between losartan-sensitive current (IC-IL) and current under control conditions. An initial value of 0.0 indicates no block. Continuous line represents monoexponential function that fits experimental data. B, Effects of E3174 on IKs recorded in another cell by same experimental protocol as in A. C and D, Effects of losartan and E3174 on voltage-dependence of IKs channel activation. Activation curves in absence (•) and in presence () of drug were fitted with a Boltzmann function. Values represent mean±SEM of 6 and 5 cells, respectively. *P<0.05 vs control.

Figure 5B shows that E3174, 1 µmol/L, was less potent than losartan at blocking I_Ks (6.5±0.7% at +50 mV, n=5, P>0.05). Moreover, this blockade was voltage-dependent, being apparent at potentials positive to 30 mV. Thus, currents elicited by pulses to -10 mV were 1.2±0.1 times higher than those obtained in the absence of drug (P>0.05). E3174 did not modify the k value of the activation curve and shifted the V_h toward more negative potentials (29.4±2.7 versus 26.1±2.3 mV, n=5, P<0.05), an effect that can account for the small increase in current amplitude observed at negative potentials (Figure 5D).

To study the effects on the activation kinetics of I_Ks, the tail amplitudes elicited on return to -30 mV after pulses to +50 mV of increasing duration (0.25 to 10 seconds) were fitted by a monoexponential function of time. By use of this procedure, the dominant time constant of the activation process of I_Ks (2615.6±236.0 ms, n=10) can be determined.⁹ Neither losartan nor E3174 modified the time course of I_Ks activation.

Finally, tail currents were adjusted by a biexponential function, and neither the fast nor the slow time constants were modified in the presence of losartan. E3174 increased both the _f (299.4±35.0 versus 379.6±43.2 ms, n=5) and _s (2029.5±113.0 versus 2288.3±32.5 ms, n=5) values, but this increase did not reach statistical significance.

Effects on Transmembrane Action Potentials
In guinea pig papillary muscles perfused with the 2 mmol/L Co²⁺-containing solution, losartan and E3174 (1 µmol/L) did not modify the resting membrane potential (-78.7±0.7 mV, n=12) or the amplitude of the action potential (106.7±2.2 mV, n=12). Losartan lengthened the action potential duration at both 50% of repolarization (APD₅₀=157.3±2.9 versus 170.0±3.8 ms, n=6, P<0.05) and 90% of repolarization (APD₉₀=204.0±3.5 versus 225.0±3.8 ms, n=6, P<0.05). E3174 prolonged the APD₉₀ from 156.2±7.2 to 167.7±9.3 ms (n=6, P<0.05) without modifying the APD₅₀ (119.2±7.7 versus 125.0±10 ms, n=6, P>0.05). In muscles perfused with 2 mmol/L Co²⁺+30 µmol/L La³⁺–containing solution, losartan did not modify the action potential characteristics, whereas E3174 shortened the APD₅₀ from 161.6±7.5 to 150.6±7.9 ms (n=6, P<0.05). Figure 6 shows superimposed action potentials recorded in the presence and in the absence of losartan or E3174 under each experimental condition.

View larger version (10K): [in this window] [in a new window]   Figure 6. Effects of losartan (L) and E3174, 1 µmol/L, on action potentials recorded in guinea pig papillary muscles perfused with 2 mmol/L Co2+ (A) or with 2 mmol/L Co2++30 µmol/L La3+ (B).

	Discussion

Top Abstract Introduction Methods Results Discussion References

 
Our results demonstrated that losartan and E3174 directly affected hKv1.5, HERG, and Ks channels. This assertion is supported by the following evidence: (1) experiments were carried out in the absence of angiotensin II, and thus, the observed effects are not the consequence of the antagonisms of its effects at the AT₁ receptor level. (2) Effects of losartan and E3174 on each current differ in potency and in voltage- and time-dependence, which is not consistent with a common mechanism of action, ie, blockade of AT₁ receptors.

Effects on hKv1.5 Currents
Losartan and E3174 transiently increased the hKv1.5 current at voltages at which the activation curve of hKv1.5 channels reached saturation. The gating modification underlying this effect is unknown and needs to be studied at the single-channel level. Under steady-state conditions, E3174- and losartan-induced block increased concomitantly with the channel opening, suggesting that both drugs bind primarily to an open and/or inactivated state of hKv1.5 channels. When almost all channels are open, a shallow voltage-dependent unblock appeared. Because losartan and E3174 are weak acids (pK_a=5.6 and 4.2, respectively), this voltage dependence can be explained by the hypothesis that the binding site is within the transmembrane electrical field and that the anionic form of both drugs reached this receptor site from the inside by crossing 20% of the membrane electrical field. In the presence of losartan and E3174, the activation curve of hKv1.5 channels became biphasic, and both drugs shifted the midpoint of the steeper component toward more negative potentials, an effect that can account for the increase in current produced at negative potentials. Because hKv1.5 channels exhibited multiple open states,¹³ the second component of the activation curve could be the consequence of a selective affinity of these drugs for the first open state, whereas strong depolarizations, which promote the transition to the second open state, will produce drug unbinding. A second possibility is that they change the voltage- and time-dependence of transitions between open states. Losartan and E3174 slowed the time course of hKv1.5 tail-current decline. This result indicates that both drugs must dissociate from the receptor before the channel can close. Finally, the blockade induced by both drugs was frequency-dependent, increasing at relevant physiologically driven frequencies.

Effects on HERG and Ks Channels
The results obtained in guinea pig myocytes in the absence of LaCl₃ suggested that losartan blocked, whereas E3174 enhanced, Kr currents, and this was confirmed in human Kr (HERG) channels. Both losartan and E3174 modified the voltage-dependence of HERG channel activation, and as was observed on hKv1.5 channels, E3174 was more potent for this effect. The negative shift of the midpoint of activation can only partially account for the E3174-induced increase on HERG currents.

Losartan-induced block on I_Ks developed faster than channel activation, which explains why it did not modify the time course of channel activation. This fast kinetics of block suggested that block already starts during conformational states that appear during transitions between the rested and the open state. Moreover, losartan apparently did not affect the time course of channel closing, which could be the consequence of its fast dissociation from the channel before it closes. In contrast, E3174-induced block on I_Ks is very small, and it developed only after channels began to open (see Figure 5B). In the presence of LaCl₃, losartan and E3174 modified the voltage-dependence of I_Ks channel activation. Indeed, losartan increased the slope factor, whereas E3174 shifted the V_h of the activation curve. The E3174-induced shift can be responsible for the small increase in the I_Ks amplitude observed at negative potentials.

Clinical Implications
Preliminary results indicated that losartan reduced the QT dispersion in patients from the ELITE study.¹⁴ We demonstrated that at therapeutic concentrations,² losartan blocks I_Ks, HERG, and hKv1.5 channels. Thus, a prolongation of the human atrial and ventricular action potentials would be expected. However, it is difficult to correlate K⁺ current blockade with action potential prolongation. In fact, hKv1.5 blockade could result in a shortening of the APD.¹⁵ In contrast, E3174 blocks hKv1.5, increases HERG, and shortens the APD₅₀ in the presence of LaCl₃, which suggests that it probably modified another current involved in ventricular repolarization. However, caution should be exerted before extrapolating the present results to explain the possible antiarrhythmic action of losartan, particularly when both losartan and E3174 are active compounds with different effects on K⁺ channels, so that the final result on APD is difficult to predict. Thus, further studies are needed to correlate our findings with their effects on human cardiac APD and to confirm its possible antiarrhythmic and/or proarrhythmic properties.

	Acknowledgments

 
This study was supported by CICYT (SAF-99-0069, SAF98-0058), CAM (08.4/0016/1998), and Spanish Society of Cardiology grants. We thank Drs M.M. Tamkun and D.J. Snyders for providing hKv1.5 channels, M. Keating and M. Sanguinetti for providing the HERG clone, J.P. Johnson and M.A. Tamargo for their help with the molecular biology, and T. Gónzalez for her helpful suggestions.

Received February 10, 1999; revision received September 13, 1999; accepted September 23, 1999.

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11. Valenzuela C, Delpón E, Franqueza L, Gay P, Pérez O, Tamargo J, Snyders DJ. Class III antiarrhythmic effects of zatebradine: time-, state-, and voltage-dependent block of hKv1.5 channels. Circulation. 1996;94:562–570.[Abstract/Free Full Text]

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