From the Departments of Internal Medicine and Pharmacology,
Cardiovascular Center and Center on Aging, University of Iowa College of
Medicine, Iowa City.
Correspondence to Donald Heistad, MD, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242. E-mail donald-heistad{at}uiowa.edu
Background—We have reported that
human mononuclear leukocytes contain large amounts of
angiotensin II (Ang II). The goal of the present study
was to test the hypothesis that Ang II is present in
monocyte/macrophages in atherosclerotic lesions.
Methods and Results—Segments of thoracic aorta and left
circumflex coronary artery were obtained from 3 groups of
cynomolgus monkeys: normal, atherosclerotic, and regression. Samples of
human coronary arterial atherosclerotic lesions
were obtained from directional atherectomy. Sections were stained for
Ang II with 3 different polyclonal rabbit anti–human Ang II antisera.
In aorta and coronary arteries from normal monkeys, there was
no or minimal anti–Ang II staining in endothelial
cells. All sections from atherosclerotic monkeys displayed discrete,
localized regions of staining for Ang II in intima-media.
Macrophages were present throughout the atherosclerotic
intima-media, and anti–Ang II staining appeared to colocalize with
macrophages. All human coronary atherectomy samples
stained positive for Ang II and macrophages. Staining for both
Ang II and macrophages was observed in vascular lesions from
all 5 monkeys after regression of atherosclerosis, but
staining was less extensive than in atherosclerotic blood vessels from
monkeys.
Conclusions—These findings suggest that Ang II is present in
atherosclerotic lesions in monkeys and humans, colocalizes with
macrophages in intima-media of atherosclerotic vessels from
monkeys, and decreases in lesions in monkeys with regression of
atherosclerosis.
© 1998 American Heart Association, Inc.
Basic Science Reports
Evidence That Macrophages in Atherosclerotic Lesions Contain Angiotensin II
Key Words: angiotensin • cells • immunohistochemistry • atherosclerosis
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