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(Circulation. 1998;98:2477-2486.)
© 1998 American Heart Association, Inc.

Basic Science Reports

Cellular Distribution of Ca²⁺ Pumps and Ca²⁺ Release Channels in Rat Cardiac Hypertrophy Induced by Aortic Stenosis

Marielle Anger, PhD; Anne-Marie Lompré, PhD; Olivier Vallot, BSc; Françoise Marotte; Lydie Rappaport, PhD; ; Jane-Lise Samuel MD, PhD

From "Gènes et protéines musculaires," Signalisation Cellulaire, Université Paris-Sud (M.A., A.-M.L., O.V.) and INSERM U127 (F.M., L.R., J.-L.S.), Paris, France.

Correspondence to A.M. Lompré, "Gènes et protéines musculaires" CNRS EP 1088, Bât 432, Université Paris-Sud, 91405 Orsay, France. E-mail anne-marie.lompre{at}egm.u-psud.fr

Background—The response of ventricular myocytes to pressure overload is heterogeneous and not spatially coordinated. We investigated whether or not the alterations in SERCA and RyR gene expression are homogeneous within the myocardium.

Methods and Results—The cellular distribution of mRNAs and proteins encoding the 2 sarco(endo)plasmic reticulum Ca²⁺-ATPase (SERCA) isoforms (SERCA 2a and 2b) and 2 Ca²⁺ release channels (the ryanodine receptor, RyR, and the IP₃ receptor, IP₃R) were analyzed by in situ hybridization and immunofluorescence, respectively. Analyses were performed during early (1 and 5 days) and late (1 month) stages of cardiac hypertrophy induced in rat by thoracic aortic stenosis (AS). The results indicated that 1 and 5 days after AS, the cellular distribution of SERCA 2a and RyR2 mRNAs in right ventricle and atrium was similar to controls but the mRNA levels appeared to decrease in some areas of the left ventricle (LV). One month after AS, the distribution of SERCA 2a mRNA and protein became heterogeneous throughout the LV, whereas RyR2 mRNA and protein levels were decreased in a homogeneous manner. SERCA 2b, poorly expressed in both cardiomyocytes and vessels of controls, was increased 4-fold 1 month after AS in coronary arteries only. In both sham (Sh) and AS, SERCA 3 and IP₃R mRNAs were mainly found in the vessels.

Conclusions—In severe hypertrophy, decreased accumulation of SERCA 2a was heterogeneous and not compensated by an induction of SERCA 2b in the cardiomyocytes. Decrease in RyR2 expression was more homogeneous and not compensated by an increased IP₃R expression.

Key Words: sarcoplasmic reticulum • hypertrophy • calcium channels • remodeling

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