From the 1. Medizinische Klinik und Deutsches Herzzentrum and Institut
für klinische Chemie und Pathobiologie (S.P., D.N., K.B.), Klinikum
rechts der Isar der Technischen Universität München, Germany.
Correspondence to Meinrad Gawaz, MD, 1. Medizinische Klinikum rechts der Isar und Deutsches Herzzentrum, Technische Universität München, Lazarettstraße 36, 80636 München, Germany. E-mail gawaz{at}dhm.mhn.de
Background—Platelet/endothelium
interaction plays an important role in the pathophysiology of
inflammation and atherosclerosis. The role of
platelets for monocyte chemotactic protein-1 (MCP-1) secretion and
surface expression of intercellular adhesion molecule-1 (ICAM-1) on
endothelial cells has been assessed.
Methods and Results—Monolayers of human umbilical vein
endothelial cells were incubated with nonstimulated or
ADP-activated platelets for 6 hours, and secretion of MCP-1
and surface expression of ICAM-1 were determined by ELISA and flow
cytometry, respectively. In the presence of ADP-activated
platelets, both MCP-1 secretion and ICAM-1 surface expression were
significantly increased compared with nonstimulated platelets
(P<0.02). Activation of the transcription factor
nuclear factor-
Conclusions—The present study indicates that
activated platelets modulate chemotactic (MCP-1) and
adhesive (ICAM-1) properties of endothelial cells via
an NF-
© 1998 American Heart Association, Inc.
Clinical Investigation and Reports
Activated Platelets Induce Monocyte Chemotactic Protein-1 Secretion and Surface Expression of Intercellular Adhesion Molecule-1 on Endothelial Cells
B (NF-B) determined by electrophoretic mobility
shift assay and B-dependent transcriptional activity was enhanced in
the presence of activated platelets. In addition,
ADP-activated platelets induced MCP-1 and ICAM-1
promoter–dependent transcription. Liposomal transfection of a
double-stranded B phosphorothioate oligonucleotide,
but not of the mutated form, inhibited MCP-1 secretion and surface
expression of ICAM-1 on activated endothelium
(P<0.05). B–dependent mechanism. Platelet-induced activation of the
NF-B system might contribute to early inflammatory events in
atherogenesis.
Key Words: platelets • endothelium • proteins • cell adhesion molecules • genes
This article has been cited by other articles:
 |
|
 |
|
  J. I. Borissoff, H. M.H. Spronk, S. Heeneman, and H. ten Cate Is thrombin a key player in the 'coagulation-atherogenesis' maze? Cardiovasc Res, June 1, 2009; 82(3): 392 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. van Gils, J. J. Zwaginga, and P. L. Hordijk Molecular and functional interactions among monocytes, platelets, and endothelial cells and their relevance for cardiovascular diseases J. Leukoc. Biol., February 1, 2009; 85(2): 195 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Gleissner, P. von Hundelshausen, and K. Ley Platelet Chemokines in Vascular Disease Arterioscler Thromb Vasc Biol, November 1, 2008; 28(11): 1920 - 1927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Hechler, M. Freund, C. Ravanat, S. Magnenat, J.-P. Cazenave, and C. Gachet Reduced Atherosclerotic Lesions in P2Y1/Apolipoprotein E Double-Knockout Mice: The Contribution of Non-Hematopoietic-Derived P2Y1 Receptors Circulation, August 12, 2008; 118(7): 754 - 763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. F. Langer, R. Haubner, B. J. Pichler, and M. Gawaz Radionuclide imaging a molecular key to the atherosclerotic plaque. J. Am. Coll. Cardiol., July 1, 2008; 52(1): 1 - 12. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Skjelland, A. Michelsen, F. Brosstad, J. L. Svennevig, R. Brucher, and D. Russell Solid Cerebral Microemboli and Cerebrovascular Symptoms in Patients With Prosthetic Heart Valves Stroke, April 1, 2008; 39(4): 1159 - 1164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Siegel-Axel, K. Daub, P. Seizer, S. Lindemann, and M. Gawaz Platelet lipoprotein interplay: trigger of foam cell formation and driver of atherosclerosis Cardiovasc Res, April 1, 2008; 78(1): 8 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Goette, A. Bukowska, U. Lendeckel, M. Erxleben, M. Hammwohner, D. Strugala, J. Pfeiffenberger, F.-W. Rohl, C. Huth, M. P.A. Ebert, et al. Angiotensin II Receptor Blockade Reduces Tachycardia-Induced Atrial Adhesion Molecule Expression Circulation, February 12, 2008; 117(6): 732 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Duygu, V. Barisik, H. Kurt, U. Turk, E. Ercan, and S. Kose Prognostic value of plasma soluble CD40 ligand in patients with chronic non-valvular atrial fibrillation Europace, February 1, 2008; 10(2): 210 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Stellos, H. Langer, K. Daub, T. Schoenberger, A. Gauss, T. Geisler, B. Bigalke, I. Mueller, M. Schumm, I. Schaefer, et al. Platelet-Derived Stromal Cell-Derived Factor-1 Regulates Adhesion and Promotes Differentiation of Human CD34+ Cells to Endothelial Progenitor Cells Circulation, January 15, 2008; 117(2): 206 - 215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Gleissner, N. Leitinger, and K. Ley Effects of Native and Modified Low-Density Lipoproteins on Monocyte Recruitment in Atherosclerosis Hypertension, August 1, 2007; 50(2): 276 - 283. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Otterdal, C. Smith, E. Oie, T. M. Pedersen, A. Yndestad, E. Stang, K. Endresen, N. O. Solum, P. Aukrust, and J. K. Damas Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes Blood, August 1, 2006; 108(3): 928 - 935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Langer, A. E. May, K. Daub, U. Heinzmann, P. Lang, M. Schumm, D. Vestweber, S. Massberg, T. Schonberger, I. Pfisterer, et al. Adherent Platelets Recruit and Induce Differentiation of Murine Embryonic Endothelial Progenitor Cells to Mature Endothelial Cells In Vitro Circ. Res., February 3, 2006; 98(2): e2 - e10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Tull, S. I. Anderson, S. C. Hughan, S. P. Watson, G. B. Nash, and G. E. Rainger Cellular Pathology of Atherosclerosis: Smooth Muscle Cells Promote Adhesion of Platelets to Cocultured Endothelial Cells Circ. Res., January 6, 2006; 98(1): 98 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Fung, R. R. Fiscus, A. P. C. Yim, G. D. Angelini, and A. A. Arifi The Potential Use of Type-5 Phosphodiesterase Inhibitors in Coronary Artery Bypass Graft Surgery Chest, October 1, 2005; 128(4): 3065 - 3073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Khallou-Laschet, G. Caligiuri, E. Tupin, A.-T. Gaston, B. Poirier, E. Groyer, D. Urbain, S. Maisnier-Patin, R. Sarkar, S.V. Kaveri, et al. Role of the Intrinsic Coagulation Pathway in Atherogenesis Assessed in Hemophilic Apolipoprotein E Knockout Mice Arterioscler Thromb Vasc Biol, August 1, 2005; 25(8): e123 - e126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Desideri, M. De Simone, L. Iughetti, T. Rosato, M. L. Iezzi, M. C. Marinucci, V. Cofini, G. Croce, G. Passacquale, S. Necozione, et al. Early Activation of Vascular Endothelial Cells and Platelets in Obese Children J. Clin. Endocrinol. Metab., June 1, 2005; 90(6): 3145 - 3152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P.J. de Winther, E. Kanters, G. Kraal, and M. H. Hofker Nuclear Factor {kappa}B Signaling in Atherogenesis Arterioscler Thromb Vasc Biol, May 1, 2005; 25(5): 904 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Yu, A. H. Rux, P. Ma, K. Bdeir, and B. S. Sachais Endothelial expression of E-selectin is induced by the platelet-specific chemokine platelet factor 4 through LRP in an NF-{kappa}B-dependent manner Blood, May 1, 2005; 105(9): 3545 - 3551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Weber Platelets and Chemokines in Atherosclerosis: Partners in Crime Circ. Res., April 1, 2005; 96(6): 612 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ungerer, M. Peluso, A. Gillitzer, S. Massberg, U. Heinzmann, C. Schulz, G. Munch, and M. Gawaz Generation of Functional Culture-Derived Platelets From CD34+ Progenitor Cells to Study Transgenes in the Platelet Environment Circ. Res., September 3, 2004; 95(5): e36 - e44. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Cabeza, Z. Li, C. Schulz, E. Kremmer, S. Massberg, A. Bultmann, and M. Gawaz Surface Expression of Collagen Receptor Fc Receptor-{gamma}/Glycoprotein VI Is Enhanced on Platelets in Type 2 Diabetes and Mediates Release of CD40 Ligand and Activation of Endothelial Cells Diabetes, August 1, 2004; 53(8): 2117 - 2121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Raymond, F. Guilbert, A. Metcalfe, G. Gevry, I. Salazkin, and O. Robledo Role of the Endothelial Lining in Recurrences After Coil Embolization: Prevention of Recanalization by Endothelial Denudation Stroke, June 1, 2004; 35(6): 1471 - 1475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gawaz Role of platelets in coronary thrombosis and reperfusion of ischemic myocardium Cardiovasc Res, February 15, 2004; 61(3): 498 - 511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schafer, C. Schulz, M. Eigenthaler, D. Fraccarollo, A. Kobsar, M. Gawaz, G. Ertl, U. Walter, and J. Bauersachs Novel role of the membrane-bound chemokine fractalkine in platelet activation and adhesion Blood, January 15, 2004; 103(2): 407 - 412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Wagner and P. C. Burger Platelets in Inflammation and Thrombosis Arterioscler Thromb Vasc Biol, December 1, 2003; 23(12): 2131 - 2137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Koh, J. Y. Ahn, S. H. Han, D. S. Kim, D. K. Jin, H. S. Kim, M.-S. Shin, T. H. Ahn, I. S. Choi, and E. K. Shin Pleiotropic effects of angiotensin II receptor blocker in hypertensive patients J. Am. Coll. Cardiol., September 3, 2003; 42(5): 905 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yoneda, K. Miura, H. Matsushima, K. Sugi, T. Murakami, K. Ouchi, K. Yamashita, H. Itoh, T. Nakazawa, M. Suzuki, et al. Aspirin inhibits Chlamydia pneumoniae-induced NF-{kappa}B activation, cyclo-oxygenase-2 expression and prostaglandin E2 synthesis and attenuates chlamydial growth J. Med. Microbiol., May 1, 2003; 52(5): 409 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nassar, B. S. Sachais, S.'e. Akkawi, M. A. Kowalska, K. Bdeir, E. Leitersdorf, E. Hiss, L. Ziporen, M. Aviram, D. Cines, et al. Platelet Factor 4 Enhances the Binding of Oxidized Low-density Lipoprotein to Vascular Wall Cells J. Biol. Chem., February 14, 2003; 278(8): 6187 - 6193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. de Lemos, D. A. Morrow, M. S. Sabatine, S. A. Murphy, C. M. Gibson, E. M. Antman, C. H. McCabe, C. P. Cannon, and E. Braunwald Association Between Plasma Levels of Monocyte Chemoattractant Protein-1 and Long-Term Clinical Outcomes in Patients With Acute Coronary Syndromes Circulation, February 11, 2003; 107(5): 690 - 695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Molero, J. Farre, A. Garcia-Mendez, P. Jimenez Mateos-Caceres, C. Carrasco Martin, I. Millas, F. Navarro, M. Cordoba, S. Casado, and A. Lopez-Farre Endothelin-1 induced proinflammatory markers in the myocardium and leukocytes of guinea-pigs: Role of glycoprotein IIB/IIIA receptors Cardiovasc Res, January 1, 2003; 57(1): 109 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-J. Neumann and N. Jander How to best counteract the enemies? By ensuring adequate oxygen delivery Eur. Heart J. Suppl., November 1, 2002; 4(suppl_G): G35 - G42. [Abstract] [PDF]
|
|
|
|
|
|
M. Werle, U. Schmal, K. Hanna, and J. Kreuzer MCP-1 induces activation of MAP-kinases ERK, JNK and p38 MAPK in human endothelial cells Cardiovasc Res, November 1, 2002; 56(2): 284 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Besta, S. Massberg, K. Brand, E. Muller, S. Page, S. Gruner, M. Lorenz, K. Sadoul, W. Kolanus, E. Lengyel, et al. Role of {beta}3-endonexin in the regulation of NF-{kappa}B-dependent expression of urokinase-type plasminogen activator receptor J. Cell Sci., October 15, 2002; 115(20): 3879 - 3888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. May, T. Kalsch, S. Massberg, Y. Herouy, R. Schmidt, and M. Gawaz Engagement of Glycoprotein IIb/IIIa ({alpha}IIb{beta}3) on Platelets Upregulates CD40L and Triggers CD40L-Dependent Matrix Degradation by Endothelial Cells Circulation, October 15, 2002; 106(16): 2111 - 2117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Massberg, K. Brand, S. Gruner, S. Page, E. Muller, I. Muller, W. Bergmeier, T. Richter, M. Lorenz, I. Konrad, et al. A Critical Role of Platelet Adhesion in the Initiation of Atherosclerotic Lesion Formation J. Exp. Med., October 7, 2002; 196(7): 887 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tanaka, T. Kuroiwa, H. Ikeuchi, F. Ota, Y. Kaneko, K. Ueki, Y. Tsukada, I. B. McInnes, D. T. Boumpas, and Y. Nojima Human Platelets Stimulate Mesangial Cells to Produce Monocyte Chemoattractant Protein-1 via the CD40/CD40 Ligand Pathway and May Amplify Glomerular Injury J. Am. Soc. Nephrol., October 1, 2002; 13(10): 2488 - 2496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kupatt, R. Wichels, J. Horstkotte, F. Krombach, H. Habazettl, and P. Boekstegers Molecular mechanisms of platelet-mediated leukocyte recruitment during myocardial reperfusion J. Leukoc. Biol., September 1, 2002; 72(3): 455 - 461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Theilmeier, C. Michiels, E. Spaepen, I. Vreys, D. Collen, J. Vermylen, and M. F. Hoylaerts Endothelial von Willebrand factor recruits platelets to atherosclerosis-prone sites in response to hypercholesterolemia Blood, May 29, 2002; 99(12): 4486 - 4493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. Sachais, A. Kuo, T. Nassar, J. Morgan, K. Kariko, K. J. Williams, M. Feldman, M. Aviram, N. Shah, L. Jarett, et al. Platelet factor 4 binds to low-density lipoprotein receptors and disrupts the endocytic itinerary, resulting in retention of low-density lipoprotein on the cell surface Blood, May 15, 2002; 99(10): 3613 - 3622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hilf, H. Singh-Jasuja, P. Schwarzmaier, C. Gouttefangeas, H.-G. Rammensee, and H. Schild Human platelets express heat shock protein receptors and regulate dendritic cell maturation Blood, May 15, 2002; 99(10): 3676 - 3682. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Salter, C. F. Krieglstein, A. C. Issekutz, and D. N. Granger Platelets modulate ischemia/reperfusion-induced leukocyte recruitment in the mesenteric circulation Am J Physiol Gastrointest Liver Physiol, December 1, 2001; 281(6): G1432 - G1439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Christen, R. J. Glynn, U. A. Ajani, D. A. Schaumberg, E. Y. Chew, J. E. Buring, J. E. Manson, and C. H. Hennekens Age-Related Maculopathy in a Randomized Trial of Low-Dose Aspirin Among US Physicians Arch Ophthalmol, August 1, 2001; 119(8): 1143 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Mazzone, S De Servi, I Mazzucchelli, I Bossi, E Ottini, M Vezzoli, F Meloni, M Lotzinker, and G Mariani Increased concentrations of inflammatory mediators in unstable angina: correlation with serum troponin T Heart, May 1, 2001; 85(5): 571 - 575. [Abstract] [Full Text]
|
|
|
|
|
|
F.-J. Neumann Optimization of microvascular reperfusion in acute myocardial infarction Eur. Heart J. Suppl., May 1, 2001; 3(suppl_A): A21 - A25. [Abstract] [PDF]
|
|
|
|
|
|
T. Dickfeld, E. Lengyel, A. E May, S. Massberg, K. Brand, S. Page, C. Thielen, K. Langenbrink, and M. Gawaz Transient interaction of activated platelets with endothelial cells induces expression of monocyte-chemoattractant protein-1 via a p38 mitogen-activated protein kinase mediated pathway: Implications for atherogenesis Cardiovasc Res, January 1, 2001; 49(1): 189 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kupatt, H. Habazettl, P. Hanusch, R. Wichels, D. Hahnel, B. F. Becker, and P. Boekstegers c7E3Fab Reduces Postischemic Leukocyte-Thrombocyte Interaction Mediated by Fibrinogen : Implications for Myocardial Reperfusion Injury Arterioscler Thromb Vasc Biol, October 1, 2000; 20(10): 2226 - 2232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fateh-Moghadam, W. Bocksch, A. Ruf, T. Dickfeld, M. Schartl, G. Pogatsa-Murray, R. Hetzer, E. Fleck, and M. Gawaz Changes in Surface Expression of Platelet Membrane Glycoproteins and Progression of Heart Transplant Vasculopathy Circulation, August 22, 2000; 102(8): 890 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Marfella, K. Esposito, R. Giunta, G. Coppola, L. De Angelis, B. Farzati, G. Paolisso, and D. Giugliano Circulating Adhesion Molecules in Humans : Role of Hyperglycemia and Hyperinsulinemia Circulation, May 16, 2000; 101(19): 2247 - 2251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Anderson Assessment and treatment of endothelial dysfunction in humans J. Am. Coll. Cardiol., September 1, 1999; 34(3): 631 - 638. [Full Text] [PDF]
|
|
|
|
|
|
M. Gawaz, F.-J. Neumann, and A. Schomig Evaluation of Platelet Membrane Glycoproteins in Coronary Artery Disease : Consequences for Diagnosis and Therapy Circulation, January 12, 1999; 99 (1): e1 - e11. [Full Text] [PDF]
|
|
|
|
|
|
T. Edgington More Cellular Signals for Atherogenesis? Circulation, September 22, 1998; 98(12): 1151 - 1152. [Full Text] [PDF]
|
|