This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tanji-Matsuba, K. Articles by Hogg, J. C. Search for Related Content PubMed PubMed Citation Articles by Tanji-Matsuba, K. Articles by Hogg, J. C. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB HYDROGEN PEROXIDE

(Circulation. 1998;97:91-98.)
© 1998 American Heart Association, Inc.

Basic Science Reports

Functional Changes in Aging Polymorphonuclear Leukocytes

Kayoko Tanji-Matsuba, MSc; Stephan F. van Eeden, MD, PhD; Yuji Saito, MD; Mitsushi Okazawa, MD; Maria E. Klut, PhD; Shizu Hayashi, PhD; ; James C. Hogg, MD, PhD

From the University of British Columbia, Pulmonary Research Laboratory, St Paul's Hospital, Vancouver, Canada.

Correspondence to Dr Stephan F. van Eeden, UBC, Pulmonary Research Laboratory, St Paul's Hospital, Vancouver, BC, Canada V6Z 1Y6. E-mail svaneeden{at}prl.pulmonary.ubc.ca

Background—Previous studies from our laboratory have shown that the expression of L-selectin on polymorphonuclear neutrophils (PMN) decreases as the cell ages in the circulation and that these older PMN have more fragmented DNA and show morphological features of apoptosis.

Methods and Results—The present study was designed to compare the functional capabilities of PMN expressing low levels of L-selectin (L-selectin^low) and the total population of PMN they were isolated from (L-selectin^mixed). The results show no difference of the baseline filamentous actin (F-actin) content between PMN expressing low and high levels of L-selectin. However, the ability of L-selectin^low PMN to assemble F-actin was impaired after stimulation by n-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP) (1 nmol/L fMLP: P<.02, 10 nmol/L fMLP: P<.01). The ability of L-selectin^low PMN to change shape when stimulated (10 nmol/L fMLP) was also decreased (P<.05). Filtration studies showed no difference in baseline deformability between L-selectin^low and L-selectin^mixed leukocytes, but the L-selectin^low cells showed a decreased ability to stiffen after fMLP stimulation (P<.05). L-selectin^low cells demonstrated a decreased ability to migrate toward a chemoattractant (1, 3, and 10 nmol/L fMLP) (P<.004) but have an enhanced ability to upregulate CD18 (P<.00002) and produce hydrogen peroxide (P<.00004).

Conclusions—We conclude that PMN undergo substantial functional changes as they age in the circulation.

Key Words: neutrophils • cells • chemotaxis • adhesion molecules • free radicals

This article has been cited by other articles:

				B. Hendey, C. L. Zhu, and S. Greenstein Fas activation opposes PMA-stimulated changes in the localization of PKC{delta}: a mechanism for reducing neutrophil adhesion to endothelial cells J. Leukoc. Biol., May 1, 2002; 71(5): 863 - 870. [Abstract] [Full Text] [PDF]

				C. Fiuza, M. Salcedo, G. Clemente, and J. M. Tellado Granulocyte Colony-Stimulating Factor Improves Deficient In Vitro Neutrophil Transendothelial Migration in Patients with Advanced Liver Disease Clin. Vaccine Immunol., March 1, 2002; 9(2): 433 - 439. [Abstract] [Full Text] [PDF]

				V. Durand, J.-O. Pers, Y. Renaudineau, A. Saraux, P. Youinou, and C. Jamin Differential effects of anti-Fc{gamma}RIIIb autoantibodies on polymorphonuclear neutrophil apoptosis and function J. Leukoc. Biol., February 1, 2001; 69(2): 233 - 240. [Abstract] [Full Text]

				S. Greenstein, J. Barnard, K. Zhou, M. Fong, and B. Hendey Fas activation reduces neutrophil adhesion to endothelial cells J. Leukoc. Biol., November 1, 2000; 68(5): 715 - 722. [Abstract] [Full Text]

				S. B. Rizoli, O. D. Rotstein, and A. Kapus Cell Volume-dependent Regulation of L-selectin Shedding in Neutrophils. A ROLE FOR p38 MITOGEN-ACTIVATED PROTEIN KINASE J. Biol. Chem., July 30, 1999; 274(31): 22072 - 22080. [Abstract] [Full Text] [PDF]

				S. F. van Eeden, J. Granton, J. M. Hards, B. Moore, and J. C. Hogg Expression of the cell adhesion molecules on leukocytes that demarginate during acute maximal exercise J Appl Physiol, March 1, 1999; 86(3): 970 - 976. [Abstract] [Full Text] [PDF]