(Circulation. 1997;96:3593-3601.)
© 1997 American Heart Association, Inc.
Articles |
Vitamins C and E Inhibit O2- Production in the Pig Coronary Artery
From the Department of Medicine (Cardiology Division), University of Washington, Seattle (B.C.B.), and the Departments of Medicine (Andreas Gruentzig Cardiovascular Center) (G.L.N., K.R., A.K., S.B.K.) and Pathology (D.S.S.), Emory University School of Medicine, Atlanta, Ga. Dr Nunes is now at Dante Pazzanese Cardiology Institute, São Paolo, Brazil.
Correspondence to Bradford C. Berk, MD, PhD, Division of Cardiology, 357710, University of Washington, Seattle, WA 98195. E-mail bcberk{at}u.washington.edu
Background We previously found in a pig coronary balloon injury model that vitamins C and E as well as probucol had beneficial effects on the vessel response to injury measured by morphometry. These effects correlated with an inhibition in the ability to oxidize LDLs ex vivo, suggesting that the morphological response was due to the antioxidant effect of the treatments.
Methods and Results In the present study, the
production of O2- by vessels 14 days
after balloon injury was determined and correlated with circulating and
tissue levels of vitamins C and E. Twenty-five domestic pigs were
divided into four groups: control (n=7), vitamin C (500 mg/d, group C,
n=6), vitamin E (1000 IU/d, group E, n=6), and vitamins C and E (500
mg/d and 1000 IU/d, group C+E, n=6). Vitamins were administered 7 days
before oversized balloon injury of the left anterior descending
coronary artery (LAD) and continued for 14 days after injury.
Vitamin C and E concentrations were determined in plasma and
lymphocytes as an index for tissue levels. Vessels were harvested after
animals were killed, and O2-
production was measured by lucigenin chemiluminescence.
O2- production by the injured LAD was
2.5-fold greater than O2- production
by the uninjured LAD or right coronary artery (RCA). The
increase in O2- was caused primarily by cells
present in the media and neointima. All vitamin-treated
groups showed significantly decreased O2-
production in both the RCA and LAD (
45% inhibition)
relative to vessels in the control, untreated group. There was a
significant correlation between LAD O2-
production and lymphocyte vitamin E levels.
Conclusions The present study is the first to show increased O2- production in injured vessels and to demonstrate that antioxidant vitamins reduce O2- production. These results suggest that beneficial effects of antioxidant vitamins in coronary artery disease are related, in part, to alterations in vessel redox state.
Key Words: antioxidants • restenosis • free radicals • vasculature
This article has been cited by other articles:
 |
|
 |
|
  E. C. Chan, S. R. Datla, R. Dilley, H. Hickey, G. R. Drummond, and G. J. Dusting Adventitial application of the NADPH oxidase inhibitor apocynin in vivo reduces neointima formation and endothelial dysfunction in rabbits Cardiovasc Res, September 1, 2007; 75(4): 710 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ohtani, K. Egashira, Y. Ihara, K. Nakano, K. Funakoshi, G. Zhao, M. Sata, and K. Sunagawa Angiotensin II Type 1 Receptor Blockade Attenuates In-Stent Restenosis by Inhibiting Inflammation and Progenitor Cells Hypertension, October 1, 2006; 48(4): 664 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Menshikov, O. Plekhanova, H. Cai, K. Chalupsky, Y. Parfyonova, P. Bashtrikov, V. Tkachuk, and B. C. Berk Urokinase Plasminogen Activator Stimulates Vascular Smooth Muscle Cell Proliferation Via Redox-Dependent Pathways Arterioscler. Thromb. Vasc. Biol., April 1, 2006; 26(4): 801 - 807. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Chen, J. F. Keaney Jr., E. Schulz, B. Levison, L. Shan, M. Sakuma, X. Zhang, C. Shi, S. L. Hazen, and D. I. Simon Decreased neointimal formation in Nox2-deficient mice reveals a direct role for NADPH oxidase in the response to arterial injury PNAS, August 31, 2004; 101(35): 13014 - 13019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Weber, Y. Taniyama, P. Rocic, P. N. Seshiah, M. A. Dechert, W. T. Gerthoffer, and K. K. Griendling Phosphoinositide-Dependent Kinase 1 and p21-Activated Protein Kinase Mediate Reactive Oxygen Species-Dependent Regulation of Platelet-Derived Growth Factor-Induced Smooth Muscle Cell Migration Circ. Res., May 14, 2004; 94(9): 1219 - 1226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z G Jin and B C Berk SOXF: redox mediators of vascular smooth muscle cell growth Heart, May 1, 2004; 90(5): 488 - 490. [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. Griendling and G. A. FitzGerald Oxidative Stress and Cardiovascular Injury: Part II: Animal and Human Studies Circulation, October 28, 2003; 108(17): 2034 - 2040. [Full Text] [PDF]
|
|
|
|
|
|
J. B. Rice, L. L. Stoll, W.-G. Li, G. M. Denning, J. Weydert, E. Charipar, W. E. Richenbacher, F. J. Miller Jr, and N. L. Weintraub Low-Level Endotoxin Induces Potent Inflammatory Activation of Human Blood Vessels: Inhibition by Statins Arterioscler. Thromb. Vasc. Biol., September 1, 2003; 23(9): 1576 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Cipollone, M Fazia, A Iezzi, B Pini, F Costantini, D De Cesare, L Paloscia, G Materazzo, E D'Annunzio, T Bucciarelli, et al. High preprocedural non-HDL cholesterol is associated with enhanced oxidative stress and monocyte activation after coronary angioplasty: possible implications in restenosis Heart, July 1, 2003; 89(7): 773 - 779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Jacobson, H. M. Dourron, J. Liu, O. A. Carretero, D. J. Reddy, T. Andrzejewski, and P. J. Pagano Novel NAD(P)H Oxidase Inhibitor Suppresses Angioplasty-Induced Superoxide and Neointimal Hyperplasia of Rat Carotid Artery Circ. Res., April 4, 2003; 92(6): 637 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. V. d'Uscio, S. Milstien, D. Richardson, L. Smith, and Z. S. Katusic Long-Term Vitamin C Treatment Increases Vascular Tetrahydrobiopterin Levels and Nitric Oxide Synthase Activity Circ. Res., January 10, 2003; 92(1): 88 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. McVeigh, P. Hamilton, M. Wilson, C. G. Hanratty, W. J. Leahey, A. B. Devine, D. G. Morgan, L. J. Dixon, and L. T. McGrath Platelet Nitric Oxide and Superoxide Release During the Development of Nitrate Tolerance: Effect of Supplemental Ascorbate Circulation, July 9, 2002; 106(2): 208 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. D. Lucca, J. T. Saari, J. C. Falcone, and D. A. Schuschke Neointima Formation in the Rat Carotid Artery Is Exacerbated by Dietary Copper Deficiency Experimental Biology and Medicine, July 1, 2002; 227(7): 487 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Aymong, M. J. Curtis, M. Youssef, M. M. Graham, L. Shewchuk, W. Leschuk, and T. J. Anderson Abciximab Attenuates Coronary Microvascular Endothelial Dysfunction After Coronary Stenting Circulation, June 25, 2002; 105(25): 2981 - 2985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Shihabi, W.-G. Li, F. J. Miller Jr., and N. L. Weintraub Antioxidant therapy for atherosclerotic vascular disease: the promise and the pitfalls Am J Physiol Heart Circ Physiol, March 1, 2002; 282(3): H797 - H802. [Full Text] [PDF]
|
|
|
|
|
|
N. L. Weintraub Nox Response to Injury Arterioscler. Thromb. Vasc. Biol., January 1, 2002; 22(1): 4 - 5. [Full Text] [PDF]
|
|
|
|
|
|
K. Szocs, B. Lassegue, D. Sorescu, L. L. Hilenski, L. Valppu, T. L. Couse, J. N. Wilcox, M. T. Quinn, J.D. Lambeth, and K. K. Griendling Upregulation of Nox-Based NAD(P)H Oxidases in Restenosis After Carotid Injury Arterioscler. Thromb. Vasc. Biol., January 1, 2002; 22(1): 21 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. V. Khan, S. Navalkar, Q. A. Khan, S. T. Rahman, and S. Parthasarathy Irbesartan, an angiotensin type 1 receptor inhibitor, regulates the vascular oxidative state in patients with coronary artery disease J. Am. Coll. Cardiol., November 15, 2001; 38(6): 1662 - 1667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Silacci, A. Desgeorges, L. Mazzolai, C. Chambaz, and D. Hayoz Flow Pulsatility Is a Critical Determinant of Oxidative Stress in Endothelial Cells Hypertension, November 1, 2001; 38(5): 1162 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Gurjar, J. DeLeon, R. V. Sharma, and R. C. Bhalla Mechanism of inhibition of matrix metalloproteinase-9 induction by NO in vascular smooth muscle cells J Appl Physiol, September 1, 2001; 91(3): 1380 - 1386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shi, S. Patel, K. L. Davenpeck, R. Niculescu, E. Rodriguez, M. G. Magno, M. L. Ormont, J. D. Mannion, and A. Zalewski Oxidative Stress and Lipid Retention in Vascular Grafts : Comparison Between Venous and Arterial Conduits Circulation, May 15, 2001; 103(19): 2408 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Miller Jr Adventitial Fibroblasts : Backstage Journeymen Arterioscler. Thromb. Vasc. Biol., May 1, 2001; 21(5): 722 - 723. [Full Text] [PDF]
|
|
|
|
|
|
Y. Shi, R. Niculescu, D. Wang, S. Patel, K. L. Davenpeck, and A. Zalewski Increased NAD(P)H Oxidase and Reactive Oxygen Species in Coronary Arteries After Balloon Injury Arterioscler. Thromb. Vasc. Biol., May 1, 2001; 21(5): 739 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Cipollone, M. Marini, M. Fazia, B. Pini, A. Iezzi, M. Reale, L. Paloscia, G. Materazzo, E. D'Annunzio, P. Conti, et al. Elevated Circulating Levels of Monocyte Chemoattractant Protein-1 in Patients With Restenosis After Coronary Angioplasty Arterioscler. Thromb. Vasc. Biol., March 1, 2001; 21(3): 327 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Orbe, J. A Rodriguez, A. Calvo, A. Grau, M. S Belzunce, D. Martinez-Caro, and J. A Paramo Vitamins C and E attenuate plasminogen activator inhibitor-1 (PAI-1) expression in a hypercholesterolemic porcine model of angioplasty Cardiovasc Res, February 1, 2001; 49(2): 484 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Ellis, R. A. Anderson, D. Lang, D. J. Blackman, R. H. K. Morris, J. Morris-Thurgood, I. F. W. McDowell, S. K. Jackson, M. J. Lewis, and M. P. Frenneaux Neutrophil superoxide anion-generating capacity, endothelial function and oxidative stress in chronic heart failure: effects of short- and long-term vitamin C therapy J. Am. Coll. Cardiol., November 1, 2000; 36(5): 1474 - 1482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-G. Jin, M. G. Melaragno, D.-F. Liao, C. Yan, J. Haendeler, Y.-A. Suh, J. D. Lambeth, and B. C. Berk Cyclophilin A Is a Secreted Growth Factor Induced by Oxidative Stress Circ. Res., October 27, 2000; 87(9): 789 - 796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Griendling, D. Sorescu, B. Lassegue, and M. Ushio-Fukai Modulation of Protein Kinase Activity and Gene Expression by Reactive Oxygen Species and Their Role in Vascular Physiology and Pathophysiology Arterioscler. Thromb. Vasc. Biol., October 1, 2000; 20(10): 2175 - 2183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Frank, S. Eguchi, T. Yamakawa, S.-i. Tanaka, T. Inagami, and E. D. Motley Involvement of Reactive Oxygen Species in the Activation of Tyrosine Kinase and Extracellular Signal-Regulated Kinase by Angiotensin II Endocrinology, September 1, 2000; 141(9): 3120 - 3126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C.P. Azevedo, M. d. A. Pedro, L. C. Souza, H. P. de Souza, M. Janiszewski, P. L. da Luz, and F. R.M. Laurindo Oxidative stress as a signaling mechanism of the vascular response to injury: The redox hypothesis of restenosis Cardiovasc Res, August 18, 2000; 47(3): 436 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. GÖRLACH, R. P. BRANDES, S. BASSUS, N. KRONEMANN, C. M. KIRCHMAIER, R. BUSSE, and V. B. SCHINI-KERTH Oxidative stress and expression of p22phox are involved in the up-regulation of tissue factor in vascular smooth muscle cells in response to activated platelets FASEB J, August 1, 2000; 14(11): 1518 - 1528. [Abstract] [Full Text]
|
|
|
|
|
|
W.-G. Li, F. J. Miller Jr, M. R. Brown, P. Chatterjee, G. R. Aylsworth, J. Shao, A. A. Spector, L. W. Oberley, and N. L. Weintraub Enhanced H2O2-Induced Cytotoxicity in "Epithelioid" Smooth Muscle Cells : Implications for Neointimal Regression Arterioscler. Thromb. Vasc. Biol., June 1, 2000; 20(6): 1473 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sinnaeve, O. Varenne, D. Collen, and S. Janssens Gene therapy in the cardiovascular system: an update Cardiovasc Res, December 1, 1999; 44(3): 498 - 506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jeserich, T. Schindler, M. Olschewski, M. Unmussig, H. Just, and U. Solzbach Vitamin C improves endothelial function of epicardial coronary arteries in patients with hypercholesterolaemia or essential hypertension--assessed by cold pressor testing Eur. Heart J., November 2, 1999; 20(22): 1676 - 1680. [Abstract] [PDF]
|
|
|
|
|
|
T. Saldeen, D. Li, and J. L. Mehta Differential effects of {alpha}- and {gamma}-tocopherol on low-density lipoprotein oxidation, superoxide activity, platelet aggregation and arterial thrombogenesis J. Am. Coll. Cardiol., October 1, 1999; 34(4): 1208 - 1215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Brown, F. J. Miller Jr, W.-G. Li, A. N. Ellingson, J. D. Mozena, P. Chatterjee, J. F. Engelhardt, R. M. Zwacka, L. W. Oberley, X. Fang, et al. Overexpression of Human Catalase Inhibits Proliferation and Promotes Apoptosis in Vascular Smooth Muscle Cells Circ. Res., September 17, 1999; 85(6): 524 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Pollman, J. L. Hall, and G. H. Gibbons Determinants of Vascular Smooth Muscle Cell Apoptosis After Balloon Angioplasty Injury : Influence of Redox State and Cell Phenotype Circ. Res., January 22, 1999; 84(1): 113 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ross Atherosclerosis -- An Inflammatory Disease N. Engl. J. Med., January 14, 1999; 340(2): 115 - 126. [Full Text] [PDF]
|
|
|
|
|
|
K. Miwa, A. Igawa, K. Nakagawa, T. Hirai, and H. Inoue Consumption of vitamin E in coronary circulation in patients with variant angina Cardiovasc Res, January 1, 1999; 41(1): 291 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Zafari, M. Ushio-Fukai, M. Akers, Q. Yin, A. Shah, D. G. Harrison, W. R. Taylor, and K. K. Griendling Role of NADH/NADPH Oxidase–Derived H2O2 in Angiotensin II–Induced Vascular Hypertrophy Hypertension, September 1, 1998; 32(3): 488 - 495. [Abstract] [Full Text] [PDF]
|
|