(Circulation. 1996;94:3109-3114.)
© 1996 American Heart Association, Inc.
Articles |
Long-term Smoking Impairs Platelet-Derived Nitric Oxide Release
the Third Department of Internal Medicine, Kurume University School of Medicine, Kurume, Japan.
Correspondence to Dr Hisao Ikeda, Third Department of Internal Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830, Japan.
Background Long-term smoking impairs endothelium-dependent vasodilation, which is mediated by nitric oxide (NO). However, it is unknown whether long-term smoking impairs the platelet-derived NO release, which regulates platelet aggregation.
Methods and Results Platelet-derived electrical current induced by collagen was measured with an NO-selective electrode in 12 smokers and 11 nonsmokers. Collagen-induced intraplatelet cGMP and platelet aggregation was measured in smokers and nonsmokers. S-nitroso-N-acetyl-dl-penicillamine, a direct NO donor, dose dependently increased in electrical current (r=.99). Collagen induced platelet aggregation and dose dependently increased electrical current (r=.94). Collagen-induced electrical current and cGMP were significantly augmented by L-arginine, a precursor of NO, and attenuated by NG-monomethyl-L-arginine, an inhibitor of NO synthesis. Significant correlation was found between collagen-induced electrical current and cGMP (r=.73). These findings indicate that the change in electrical current reflects the NO release through the L-arginine–NO pathway in platelets. Collagen-induced electrical current (6.7 versus 13.8 pA; P<.001) and cGMP (1.2 versus 3.0 pmol/109 platelets; P<.005) were significantly lower in smokers than in nonsmokers. Although L-arginine increased cGMP levels in both smokers and nonsmokers, the level was still lower in smokers than in nonsmokers. The inhibitory effect of L-arginine on collagen-induced platelet aggregation was significantly lower in smokers than in nonsmokers (P<.05).
Conclusions These findings provide evidence that platelet-derived NO release is significantly impaired in long-term smokers, resulting in the augmentation of platelet aggregability.
Key Words: smoking • risk factors • platelets • platelet-derived factors • collagen
This article has been cited by other articles:
 |
|
 |
|
  R. O. Wein Preoperative Smoking Cessation: Impact on Perioperative and Long-term Complications Arch Otolaryngol Head Neck Surg, June 1, 2009; 135(6): 597 - 601. [Full Text] [PDF]
|
|
|
|
 |
|
 G. De Luca Adjunctive antithrombotic therapy during primary percutaneous coronary intervention Eur. Heart J. Suppl., December 1, 2008; 10(suppl_J): J2 - J14. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tseeng and R. Arora Aspirin Resistance: Biological and Clinical Implications Journal of Cardiovascular Pharmacology and Therapeutics, March 1, 2008; 13(1): 5 - 12. [Abstract] [PDF]
|
|
|
|
 |
|
 E. Gkaliagkousi, J. Ritter, and A. Ferro Platelet-Derived Nitric Oxide Signaling and Regulation Circ. Res., September 28, 2007; 101(7): 654 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Haramaki, H. Ikeda, K. Takenaka, A. Katoh, R. Sugano, S.-i. Yamagishi, H. Matsuoka, and T. Imaizumi Fluvastatin Alters Platelet Aggregability in Patients With Hypercholesterolemia: Possible Improvement of Intraplatelet Redox Imbalance via HMG-CoA Reductase Arterioscler. Thromb. Vasc. Biol., June 1, 2007; 27(6): 1471 - 1477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shimasaki, Y. Saito, M. Yoshimura, S. Kamitani, Y. Miyamoto, I. Masuda, M. Nakayama, Y. Mizuno, H. Ogawa, H. Yasue, et al. The Effects of Long-term Smoking on Endothelial Nitric Oxide Synthase mRNA Expression in Human Platelets as Detected With Real-time Quantitative RT-PCR Clinical and Applied Thrombosis/Hemostasis, January 1, 2007; 13(1): 43 - 51. [Abstract] [PDF]
|
|
|
|
|
|
S. R. Willoughby, S. Stewart, A. S. Holmes, Y. Y. Chirkov, and J. D. Horowitz Platelet Nitric Oxide Responsiveness: A Novel Prognostic Marker in Acute Coronary Syndromes Arterioscler. Thromb. Vasc. Biol., December 1, 2005; 25(12): 2661 - 2666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Morita, H. Ikeda, N. Haramaki, H. Eguchi, and T. Imaizumi Only two-week smoking cessation improves platelet aggregability and intraplatelet redox imbalance of long-term smokers J. Am. Coll. Cardiol., February 15, 2005; 45(4): 589 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Stocker and J. F. Keaney Jr. Role of Oxidative Modifications in Atherosclerosis Physiol Rev, October 1, 2004; 84(4): 1381 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Ambrose and R. S. Barua The pathophysiology of cigarette smoking and cardiovascular disease: An update J. Am. Coll. Cardiol., May 19, 2004; 43(10): 1731 - 1737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tsiara, M. Elisaf, and D. P. Mikhailidis Influence of Smoking on Predictors of Vascular Disease Angiology, September 1, 2003; 54(5): 507 - 530. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Kanaya, H. Ikeda, N. Haramaki, T. Murohara, and T. Imaizumi Intraplatelet Tetrahydrobiopterin Plays an Important Role in Regulating Canine Coronary Arterial Thrombosis by Modulating Intraplatelet Nitric Oxide and Superoxide Generation Circulation, November 13, 2001; 104(20): 2478 - 2484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takajo, H. Ikeda, N. Haramaki, T. Murohara, and T. Imaizumi Augmented oxidative stress of platelets in chronic smokers: Mechanisms of impaired platelet-derived nitric oxide bioactivity and augmented platelet aggregability J. Am. Coll. Cardiol., November 1, 2001; 38(5): 1320 - 1327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Haramaki, H. Ikeda, Y. Takajo, A. Katoh, S. Kanaya, S. Shintani, R. Haramaki, T. Murohara, and T. Imaizumi Long-Term Smoking Causes Nitroglycerin Resistance in Platelets by Depletion of Intraplatelet Glutathione Arterioscler. Thromb. Vasc. Biol., November 1, 2001; 21(11): 1852 - 1856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Loscalzo Nitric Oxide Insufficiency, Platelet Activation, and Arterial Thrombosis Circ. Res., April 27, 2001; 88(8): 756 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ikeda, Y. Takajo, T. Murohara, K. Ichiki, H. Adachi, N. Haramaki, A. Katoh, and T. Imaizumi Platelet-Derived Nitric Oxide and Coronary Risk Factors Hypertension, April 1, 2000; 35(4): 904 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sztajzel, F. Mach, and A. Righetti Current concepts in medicine: Role of the vascular endothelium in patients with angina pectoris or acute myocardial infarction with normal coronary arteries Postgrad. Med. J., January 1, 2000; 76(891): 16 - 21. [Abstract] [Full Text]
|
|
|
|
|
|
J. H. Markovitz, L. Tolbert, and S. E. Winders Increased Serotonin Receptor Density and Platelet GPIIb/IIIa Activation Among Smokers Arterioscler. Thromb. Vasc. Biol., March 1, 1999; 19(3): 762 - 766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Freedman, B. Ting, B. Hankin, J. Loscalzo, J. F. Keaney Jr, and J. A. Vita Impaired Platelet Production of Nitric Oxide Predicts Presence of Acute Coronary Syndromes Circulation, October 13, 1998; 98(15): 1481 - 1486. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T Powell Vascular damage from smoking: disease mechanisms at the arterial wall Vascular Medicine, February 1, 1998; 3(1): 21 - 28. [Abstract] [PDF]
|
|