(Circulation. 1996;94:3074-3078.)
© 1996 American Heart Association, Inc.
Articles |
Common Mutation in Methylenetetrahydrofolate Reductase
Correlation With Homocysteine Metabolism and Late-Onset Vascular Disease
the Division of Hematology and Medical Oncology, Department of Medicine (T.G.D., A.E., J.M., W.D.H.); the Division of Vascular Surgery, Department of Surgery (L.M.T.); and the Division of Laboratory Medicine, Department of Pathology (T.G.D., A.E., A.S., R.D.P.), Oregon Health Sciences University, Portland.
Correspondence to Thomas G. Deloughery, MD, Oregon Health Sciences University, Hematology L471, 3181 SW Sam Jackson Park Rd, Portland, OR 97201-3098. E-mail delought@ccmail.ohsu.edu.
Background Increased homocysteine levels are a risk factor for atherosclerosis and its sequelae. A common genetic mutation in methylenetetrahydrofolate reductase (MTHFR), an enzyme required for efficient homocysteine metabolism, creates a thermolabile enzyme with reduced activity. We determined the prevalence of this mutation in many subjects with and without vascular disease and related it to homocysteine and folate levels.
Methods and Results DNA from 247 older subjects with vascular disease and 594 healthy subjects without vascular disease (in three different control groups) was screened for the MTHFR 677 C-to-T mutation. Within each group, 9% to 17% of the subjects were homozygous for this mutation, and the mutant allele frequency was 31% to 39%. The genotype distributions, homozygote frequencies, and allele frequencies did not differ significantly between the study groups. In the vascular disease subjects, despite significantly lower folate levels in MTHFR homozygotes, there was no significant difference in homocysteine levels among the MTHFR genotype groups. The negative slope of the regression line relating homocysteine and folate was significantly steeper for those with a homozygous MTHFR mutation compared with those without this mutation.
Conclusions Although the thermolabile MTHFR mutation is very common, it does not appear to be a significant genetic risk factor for typical late-onset vascular disease. Because MTHFR homozygotes have increased homocysteine with low folate levels, this mutation may contribute to early-onset or familial vascular disease. The genotype dependence of the folate-homocysteine correlation further suggests that homozygotes for this mutation may have both an exaggerated hyperhomocysteinemic response to folic acid depletion and a better response to folic acid therapy.
Key Words: metabolism • arteriosclerosis • risk factors • diet
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