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(Circulation. 1995;92:2666-2675.)
© 1995 American Heart Association, Inc.

Articles

Long-term Treatment With Angiotensin I–Converting Enzyme Inhibitors Attenuates the Loss of Cardiac ß-Adrenoceptor Responses in Rats With Chronic Heart Failure

Atsushi Sanbe; Satoshi Takeo

From the Department of Pharmacology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.

Correspondence to Satoshi Takeo, PhD, Department of Pharmacology, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo, 192-03 Japan.

Background Cardiac contractile force in response to ß-adrenoceptor agonists and ß-adrenergic receptor density are decreased in failing human hearts. The effects of angiotensin I–converting enzyme (ACE) inhibitor on cardiac responsiveness to ß-adrenergic stimulation in failing hearts are not established. The present study was undertaken to determine whether ACE inhibitor may improve cardiac ß-adrenergic responsiveness in animals with chronic heart failure (CHF).

Methods and Results CHF was induced by left coronary artery ligation in rats. Cardiac output and stroke volume indices decreased 12 weeks after the operation. In sham-operated rats, dobutamine and isoprenaline increased cardiac output and stroke volume indices. In contrast, cardiac output and stroke volume responses to dobutamine and isoprenaline were severely blunted in the CHF rat. Cardiac ß₁-adrenergic receptor density was decreased while its dissociation constant (K_d) was not altered in the viable tissue of the left ventricle of the CHF rat, which is consistent with ß-adrenergic receptor downregulation. Cardiac norepinephrine content decreased in the CHF rats. Rats were treated orally with ACE inhibitors, 3 mg/kg trandolapril or 10 mg/kg enalapril once daily, or 5 mg/kg captopril twice daily from the 2nd to the 12th weeks after the operation. Treatment with ACE inhibitors attenuated the reduction in cardiac output and stroke volume indices and improved the inotropic response to dobutamine and isoprenaline and reversed partially the cardiac norepinephrine content in the CHF rat. ACE inhibitor treatment also attenuated the reduction in ß₁-adrenergic receptor density in the viable tissue of the left ventricle of the CHF rat.

Conclusions The results suggest that ACE inhibitor treatment attenuates the blunting of cardiac responses to ß-adrenergic agonists in the CHF rat and that one of the mechanisms underlying this effect is prevention of cardiac ß₁-adrenergic receptor downregulation.

Key Words: angiotensin • receptors, adrenergic, beta • heart failure

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