Circulation, Vol 80, 1347-1353, Copyright © 1989 by American Heart Association
DA Dichek, RF Neville, JA Zwiebel, SM Freeman, MB Leon and WF Anderson
The use of intravascular stents may be limited by both local thrombosis and
restenosis due to intimal proliferation. In an effort to provide solutions
to these problems, we seeded stents with genetically engineered endothelial
cells in vitro. Using retroviral-mediated gene transfer, we inserted the
gene for either bacterial beta-galactosidase or human tissue-type
plasminogen activator (t-PA) into cultured sheep endothelial cells. The
endothelial cells were seeded onto stainless steel stents and grown until
the stents were covered. Expression of intracellular beta-galactosidase and
high level secretion of t-PA were demonstrated both before and after the
transduced cells were seeded onto the stents. Eight stents were expanded by
in vitro balloon inflation, with observation of the seeded endothelial
layer both prior to and after expansion. Most of the endothelial cells
remained on the stents after balloon inflation. We conclude that
intravascular stents can be coated with a layer of genetically engineered
endothelial cells that can be either specifically labeled or made to
secrete high levels of a therapeutic protein. Much of the layer of
genetically engineered cells remains after the expansion of the stent in
vitro. In vivo implantation of stents coated with genetically engineered
endothelial cells may allow 1) introduction of genetically engineered
endothelial cells directly into the vascular wall and 2) improvement of
stent function through localized delivery of anticoagulant, thrombolytic,
or antiproliferative molecules.
ARTICLES
Seeding of intravascular stents with genetically engineered endothelial cells
Laboratory of Molecular Hematology, National Institutes of Health, Bethesda, Maryland 20892.
This article has been cited by other articles:
 |
|
 |
|
  S. V. Pislaru, A. Harbuzariu, R. Gulati, T. Witt, N. P. Sandhu, R. D. Simari, and G. S. Sandhu Magnetically Targeted Endothelial Cell Localization in Stented Vessels J. Am. Coll. Cardiol., November 7, 2006; 48(9): 1839 - 1845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. Melo, M. Gnecchi, A. S. Pachori, D. Kong, K. Wang, X. Liu, R. E. Pratt, and V. J. Dzau Endothelium-Targeted Gene and Cell-Based Therapies for Cardiovascular Disease Arterioscler Thromb Vasc Biol, October 1, 2004; 24(10): 1761 - 1774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.G Melo, M Gnecchi, A.S Pachori, K Wang, and V.J Dzau Gene- and cell-based therapies for cardiovascular diseases: current status and future directions Eur. Heart J. Suppl., September 1, 2004; 6(suppl_E): E24 - E35. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. H. Walter, M. Cejna, L. Diaz-Sandoval, S. Willis, L. Kirkwood, P. W. Stratford, A. B. Tietz, R. Kirchmair, M. Silver, C. Curry, et al. Local Gene Transfer of phVEGF-2 Plasmid by Gene-Eluting Stents: An Alternative Strategy for Inhibition of Restenosis Circulation, July 6, 2004; 110(1): 36 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ribourtout and J. Raymond Gene Therapy and Endovascular Treatment of Intracranial Aneurysms Stroke, March 1, 2004; 35(3): 786 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. S. Katusic, N. M. Caplice, and K. A. Nath Nitric Oxide Synthase Gene Transfer as a Tool to Study Biology of Endothelial Cells Arterioscler Thromb Vasc Biol, November 1, 2003; 23(11): 1990 - 1994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. F. Akowuah, P. J. Sheridan, G. J. Cooper, and C. Newman Preventing saphenous vein graft failure: does gene therapy have a role? Ann. Thorac. Surg., September 1, 2003; 76(3): 959 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. D MacNeill, I. Pomerantseva, H. C Lowe, S. N Oesterle, and J. P Vacanti Toward a new blood vessel Vascular Medicine, August 1, 2002; 7(3): 241 - 246. [Abstract] [PDF]
|
|
|
|
|
|
D. W. Losordo and J. M. Isner Vascular endothelial growth factor-induced angiogenesis: crouching tiger or hidden dragon? J. Am. Coll. Cardiol., June 15, 2001; 37(8): 2131 - 2135. [Full Text] [PDF]
|
|
|
|
|
|
H. Ooboshi, S. Ibayashi, J. Takada, H. Yao, T. Kitazono, and M. Fujishima Adenovirus-Mediated Gene Transfer to Ischemic Brain : Ischemic Flow Threshold for Transgene Expression Stroke, April 1, 2001; 32(4): 1043 - 1047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Meyerson, C. L. Skelly, M. A. Curi, and L. B. Schwartz Gene Therapy for Cardiovascular Disease Seminars in Cardiothoracic and Vascular Anesthesia, November 1, 2000; 4(4): 289 - 300. [Abstract] [PDF]
|
|
|
|
|
|
D. S Ettenson and E. R Edelman Local drug delivery: an emerging approach in the treatment of restenosis Vascular Medicine, May 1, 2000; 5(2): 97 - 102. [Abstract] [PDF]
|
|
|
|
|
|
M. O Hiltunen, M. P Turunen, M. Laitinen, and S. Yla-Herttuala Insights into the molecular pathogenesis of atherosclerosis and therapeutic strategies using gene transfer Vascular Medicine, February 1, 2000; 5(1): 41 - 48. [Abstract] [PDF]
|
|
|
|
 |
|
 H. M. Nugent, C. Rogers, and E. R. Edelman Endothelial Implants Inhibit Intimal Hyperplasia After Porcine Angioplasty Circ. Res., March 5, 1999; 84(4): 384 - 391. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Basile-Borgia, J. H Abel, and H. Mahloogi Molecular advances in cardiac and cardiovascular disease Perfusion, March 1, 1999; 14(2): 89 - 99. [Abstract] [PDF]
|
|
|
|
 |
|
 J. M. Waugh, M. Kattash, J. Li, E. Yuksel, M. D. Kuo, M. Lussier, A. B. Weinfeld, R. Saxena, E. D. Rabinovsky, S. Thung, et al. Gene therapy to promote thromboresistance: Local overexpression of tissue plasminogen activator to prevent arterial thrombosis in an in vivo rabbit model PNAS, February 2, 1999; 96(3): 1065 - 1070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Waugh, E. Yuksel, J. Li, M. D. Kuo, M. Kattash, R. Saxena, R. Geske, S. N. Thung, S. M. Shenaq, and S. L. C. Woo Local Overexpression of Thrombomodulin for In Vivo Prevention of Arterial Thrombosis in a Rabbit Model Circ. Res., January 22, 1999; 84(1): 84 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Topol and P. W. Serruys Frontiers in Interventional Cardiology Circulation, October 27, 1998; 98(17): 1802 - 1820. [Full Text] [PDF]
|
|
|
|
|
|
O. F. Bertrand, R. Sipehia, R. Mongrain, J. Rodes, J.-C. Tardif, L. Bilodeau, G. Cote, and M. G. Bourassa Biocompatibility aspects of new stent technology J. Am. Coll. Cardiol., September 1, 1998; 32(3): 562 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Van Belle, C. Bauters, T. Asahara, and J. M. Isner Endothelial regrowth after arterial injury: from vascular repair to therapeutics Cardiovasc Res, April 1, 1998; 38(1): 54 - 68. [Full Text] [PDF]
|
|
|
|
|
|
S. Baek and K. L. March Gene Therapy for Restenosis : Getting Nearer the Heart of the Matter Circ. Res., February 23, 1998; 82(3): 295 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Waltenberger Modulation of Growth Factor Action : Implications for the Treatment of Cardiovascular Diseases Circulation, December 2, 1997; 96(11): 4083 - 4094. [Abstract] [Full Text]
|
|
|
|
|
|
L. J Feldman and G. Steg Optimal techniques for arterial gene transfer Cardiovasc Res, September 1, 1997; 35(3): 391 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Brieger and E. Topol Local drug delivery systems and prevention of restenosis Cardiovasc Res, September 1, 1997; 35(3): 405 - 413. [Full Text] [PDF]
|
|
|
|
|
|
G. Vassalli and D. A Dichek Gene therapy for arterial thrombosis Cardiovasc Res, September 1, 1997; 35(3): 459 - 469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S Lewis, M. Y Flugelman, A. Weisz, I. Keren-Tal, and W. Schaper Angiogenesis by gene therapy: a new horizon for myocardial revascularization? Cardiovasc Res, September 1, 1997; 35(3): 490 - 497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Carmeliet, J.-M. Stassen, I. Van Vlaenderen, R. S. Meidell, D. Collen, and R. D. Gerard Adenovirus-Mediated Transfer of Tissue-Type Plasminogen Activator Augments Thrombolysis in Tissue-Type Plasminogen Activator-Deficient and Plasminogen Activator Inhibitor-1-Overexpressing Mice Blood, August 15, 1997; 90(4): 1527 - 1534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Muhonen, H. Ooboshi, M. J. Welsh, B. L. Davidson, and D. D. Heistad Gene Transfer to Cerebral Blood Vessels After Subarachnoid Hemorrhage Stroke, April 1, 1997; 28(4): 822 - 829. [Abstract] [Full Text]
|
|
|
|
|
|
E. Van Belle, F. O. Tio, T. Couffinhal, L. Maillard, J. Passeri, and J. M. Isner Stent Endothelialization: Time Course, Impact of Local Catheter Delivery, Feasibility of Recombinant Protein Administration, and Response to Cytokine Expedition Circulation, January 21, 1997; 95(2): 438 - 448. [Abstract] [Full Text]
|
|
|
|
|
|
C. Rogers, S. Parikh, P. Seifert, and E. R. Edelman Endogenous Cell Seeding: Remnant Endothelium After Stenting Enhances Vascular Repair Circulation, December 1, 1996; 94(11): 2909 - 2914. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. R. Makkar, N. Eigler, F. Litvack, and J. S. Forrester Prevention of Restenosis by Local Drug Delivery Journal of Cardiovascular Pharmacology and Therapeutics, April 1, 1996; 1(2): 177 - 188. [Abstract] [PDF]
|
|
|
|
|
|
P. F. Dunn, K. D. Newman, M. Jones, I. Yamada, V. Shayani, R. Virmani, and D. A. Dichek Seeding of Vascular Grafts With Genetically Modified Endothelial Cells : Secretion of Recombinant TPA Results in Decreased Seeded Cell Retention In Vitro and In Vivo Circulation, April 1, 1996; 93(7): 1439 - 1446. [Abstract] [Full Text]
|
|
|
|
|
|
D. A. Dichek, J. Anderson, A. B. Kelly, S. R. Hanson, and L. A. Harker Enhanced In Vivo Antithrombotic Effects of Endothelial Cells Expressing Recombinant Plasminogen Activators Transduced With Retroviral Vectors Circulation, January 15, 1996; 93(2): 301 - 309. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Schnaper, J. Kopp, A. Poncelet, S. Hubchak, W. Stetler-Stevenson, P. Klotman, and H. Kleinman Increased expression of extracellular matrix proteins and decreased expression of matrix proteases after serial passage of glomerular mesangial cells J. Cell Sci., January 10, 1996; 109(10): 2521 - 2528. [Abstract] [PDF]
|
|
|
|
|
|
R. T. Rowland, J. C. Cleveland Jr, X. Meng, A. H. Harken, and J. M. Brown Potential Gene Therapy Strategies in the Treatment of Cardiovascular Disease Ann. Thorac. Surg., September 1, 1995; 60(3): 721 - 728. [Abstract] [Full Text]
|
|
|
|
|
|
H. Ooboshi, M. J. Welsh, C. D. Rios, B. L. Davidson, and D. D. Heistad Adenovirus-Mediated Gene Transfer In Vivo to Cerebral Blood Vessels and Perivascular Tissue Circ. Res., July 1, 1995; 77(1): 7 - 13. [Abstract] [Full Text]
|
|
|
|
|
|
T. Asahara, C. Bauters, C. Pastore, M. Kearney, S. Rossow, S. Bunting, N. Ferrara, J. F. Symes, and J. M. Isner Local Delivery of Vascular Endothelial Growth Factor Accelerates Reendothelialization and Attenuates Intimal Hyperplasia in Balloon-Injured Rat Carotid Artery Circulation, June 1, 1995; 91(11): 2793 - 2801. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Fasol, P. Zilla, P. Preiss, U. von Oppell, J. Odell, and B. Reichart Allogenic, Multidonor In Vitro Endothelialization of Small Diameter PTFE Grafts in Baboons Vascular and Endovascular Surgery, January 1, 1991; 25(1): 64 - 71. [Abstract] [PDF]
|
|
|
|
 |
|
 E. Nabel, G Plautz, and G. Nabel Site-specific gene expression in vivo by direct gene transfer into the arterial wall Science, September 14, 1990; 249(4974): 1285 - 1288. [Abstract] [PDF]
|
|