This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gerrard, J. M. Articles by Rowe, R. Search for Related Content PubMed PubMed Citation Articles by Gerrard, J. M. Articles by Rowe, R.

Circulation, Vol 79, 29-38, Copyright © 1989 by American Heart Association

ARTICLES

In vivo measurement of thromboxane B2 and 6-keto-prostaglandin F1 alpha in humans in response to a standardized vascular injury and the influence of aspirin

JM Gerrard, S Taback, S Singhroy, JC Docherty, I Kostolansky, A McNicol, NL Kobrinsky, JK McKenzie and R Rowe
Department of Pediatrics, University of Manitoba, Winnipeg, Canada.

The effects of smoking, aspirin ingestion, and sex differences on bleeding times and bleeding time thromboxane B2 and 6-keto- prostaglandin (PG)F1 alpha production were examined. Nonsmoking men produced more thromboxane B2 (3.99 +/- 0.76 ng/ml) than nonsmoking women (2.13 +/- 0.24 ng/ml). Female smokers produced more thromboxane B2 (5.01 +/- 0.97 ng/ml) than nonsmoking women. Twenty-four hours after a single dose of 600 mg aspirin, in vitro production of thromboxane B2 in response to collagen fell by 95%, whereas in vivo production of thromboxane B2 and 6-keto-PGF1 alpha in bleeding time blood fell by 87% and 66%, respectively. Subjects with the lowest absolute levels of thromboxane B2 24 hours after aspirin were also those with the longest postaspirin bleeding times. Recovery of 6-keto-PGF1 alpha production was faster than recovery of thromboxane B2 production, but 6-keto-PGF1 alpha production for most subjects was still below basal 72 hours after aspirin. The influence of two different doses of long-term aspirin (80 mg every other day and 325 mg daily) on the in vivo production of thromboxane B2 and 6-keto-PGF1 alpha was studied in normals and diabetics. After 14 days of 80 mg aspirin every other day, thromboxane B2 and 6-keto-PGF1 alpha production were both substantially inhibited (93% and 78%, respectively). After 14 days of 325 mg aspirin daily, thromboxane B2 production was similarly substantially inhibited (93%), whereas 6-keto-PGF1 alpha was significantly less affected (only 45% inhibition). Study of a second group of five normal subjects confirmed that 6-keto-PGF1 alpha production was significantly inhibited 24 hours after the first dose of 325 mg aspirin but was not significantly less than basal after 14 days of 325 mg aspirin. The results suggest that 325 mg aspirin daily is more antithrombotic compared with 80 mg every other day due to the superior preservation of prostacyclin production.

This article has been cited by other articles:

				E. Tuleja, F. Mejza, A. Cmiel, and A. Szczeklik Effects of Cyclooxygenases Inhibitors on Vasoactive Prostanoids and Thrombin Generation at the Site of Microvascular Injury in Healthy Men Arterioscler. Thromb. Vasc. Biol., June 1, 2003; 23(6): 1111 - 1115. [Abstract] [Full Text] [PDF]

				A. Weltermann, M. Wolzt, K. Petersmann, C. Czerni, U. Graselli, K. Lechner, and P. A. Kyrle Large Amounts of Vascular Endothelial Growth Factor at the Site of Hemostatic Plug Formation In Vivo Arterioscler. Thromb. Vasc. Biol., July 1, 1999; 19(7): 1757 - 1760. [Abstract] [Full Text] [PDF]

				S. F. Khuri, N. Healey, H. MacGregor, M. R. Barnard, I. O. Szymanski, V. Birjiniuk, A. D. Michelson, D. R. Gagnon, and C. R. Valeri COMPARISON OF THE EFFECTS OF TRANSFUSIONS OF CRYOPRESERVED AND LIQUID-PRESERVED PLATELETS ON HEMOSTASIS AND BLOOD LOSS AFTER CARDIOPULMONARY BYPASS J. Thorac. Cardiovasc. Surg., January 1, 1999; 117(1): 172 - 185. [Abstract] [Full Text] [PDF]

				K. Krejcy, L. Schmetterer, J. Kastner, M. Nieszpaur-Los, B. Monitzer, W. Schutz, H.-G. Eichler, and P. A. Kyrle Role of Nitric Oxide in Hemostatic System Activation In Vivo in Humans Arterioscler. Thromb. Vasc. Biol., November 1, 1995; 15(11): 2063 - 2067. [Abstract] [Full Text]

				S. F. Khuri, C. R. Valeri, J. Loscalzo, M. J. Weinstein, V. Birjiniuk, N. A. Healey, H. MacGregor, M. Doursounian, and M. A. Zolkewitz Heparin Causes Platelet Dysfunction and Induces Fibrinolysis Before Cardiopulmonary Bypass Ann. Thorac. Surg., October 1, 1995; 60(4): 1008 - 1014. [Abstract] [Full Text]

				S. Eichinger, M. Wolzt, B. Schneider, M. Nieszpaur-Los, H. Heinrichs, K. Lechner, H.-G. Eichler, and P. A. Kyrle Effects of Recombinant Hirudin (r-Hirudin, HBW 023) on Coagulation and Platelet Activation In Vivo : Comparison With Unfractionated Heparin and a Low-Molecular-Weight Heparin Preparation (Fragmin) Arterioscler. Thromb. Vasc. Biol., July 1, 1995; 15(7): 886 - 892. [Abstract] [Full Text]