Circulation, Vol 75, 292-298, Copyright © 1987 by American Heart Association
P Golino, PR Maroko and TE Carew
The goal of this study was to determine the effects of acute
hypercholesterolemia on the evolution of myocardial infarction in a
preparation of coronary occlusion-reperfusion. New Zealand white rabbits
were fed a 2% cholesterol-enriched diet for 3 days (plasma cholesterol 329
+/- 70 mg/dl), or maintained on the control diet (plasma cholesterol 67 +/-
12 mg/dl). Temporary (30 min) coronary artery occlusion was performed in
open-chest rabbits with a suture snare. The snare was released to permit
reperfusion. When the animals were killed 5.5 hr later, left ventricles
were cut into 3 mm slices. Infarct size was determined by planimetry of
tetrazolium-stained slices while the area at risk of infarction
(hypoperfused zone) was determined by planimetry of the "cold spots" on
autoradiograms of the slices that contained 99m Tc-labeled microspheres
that had been injected 1 min after occlusion. Infarct size, expressed as
percent of the hypoperfused zone, was 42.8 +/- 1.3% (n = 10) in the control
group and was increased by approximately 100% in cholesterol-fed animals to
83.7 +/- 2.0% (n = 10, p less than .001). To test the hypothesis that
vascular obstruction (no reflow) might account for the larger infarct size,
thioflavin S was injected immediately before the animals were killed to
demarcate perfused myocardium in three additional groups of animals:
standard chow-fed rabbits (n = 5), cholesterol-fed rabbits (n = 5), and
standard chow-fed rabbits that, in addition, received an infusion of
isoproterenol (0.1 microgram/kg/min, n = 6), an intervention believed to
increase infarct size through a mechanism not dependent on the no- reflow
phenomenon.(ABSTRACT TRUNCATED AT 250 WORDS)
ARTICLES
The effect of acute hypercholesterolemia on myocardial infarct size and the no-reflow phenomenon during coronary occlusion-reperfusion
This article has been cited by other articles:
 |
|
 |
|
  P. Ferdinandy, R. Schulz, and G. F. Baxter Interaction of Cardiovascular Risk Factors with Myocardial Ischemia/Reperfusion Injury, Preconditioning, and Postconditioning Pharmacol. Rev., December 1, 2007; 59(4): 418 - 458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Galinanes and A. G Fowler Role of clinical pathologies in myocardial injury following ischaemia and reperfusion Cardiovasc Res, February 15, 2004; 61(3): 512 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Reffelmann and R. A. Kloner Is microvascular protection by cariporide and ischemic preconditioning causally linked to myocardial salvage? Am J Physiol Heart Circ Physiol, April 1, 2003; 284(4): H1134 - H1141. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Genda, T. Miura, T. Miki, Y. Ichikawa, and K. Shimamoto KATP channel opening is an endogenous mechanism of protection against the no-reflow phenomenon but its function is compromised by hypercholesterolemia J. Am. Coll. Cardiol., October 2, 2002; 40(7): 1339 - 1346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Scalia, M. E. Gooszen, S. P. Jones, M. Hoffmeyer, D. M. Rimmer III, S. D. Trocha, P. L. Huang, M. B. Smith, A. M. Lefer, and D. J. Lefer Simvastatin Exerts Both Anti-inflammatory and Cardioprotective Effects in Apolipoprotein E-Deficient Mice Circulation, May 29, 2001; 103(21): 2598 - 2603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Li, S. Tokuno, P. Tahepold, J. Vaage, C. Lowbeer, and G. Valen Preconditioning protects the severely atherosclerotic mouse heart Ann. Thorac. Surg., April 1, 2001; 71(4): 1296 - 1303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Golino, M. Ragni, P. Cirillo, A. Scognamiglio, A. Ravera, C. Buono, A. Guarino, O. Piro, C. Lambiase, F. Botticella, et al. Recombinant human, active site-blocked factor VIIa reduces infarct size and no-reflow phenomenon in rabbits Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1507 - H1516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hoshida, N. Yamashita, K. Otsu, T. Kuzuya, and M. Hori Cholesterol feeding exacerbates myocardial injury in Zucker diabetic fatty rats Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H256 - H262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Girod, S. P. Jones, N. Sieber, T. Y. Aw, and D. J. Lefer Effects of Hypercholesterolemia on Myocardial Ischemia-Reperfusion Injury in LDL Receptor-Deficient Mice Arterioscler. Thromb. Vasc. Biol., November 1, 1999; 19(11): 2776 - 2781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Jones, W. G. Girod, D. N. Granger, A. J. Palazzo, and D. J. Lefer Reperfusion injury is not affected by blockade of P-selectin in the diabetic mouse heart Am J Physiol Heart Circ Physiol, August 1, 1999; 277(2): H763 - H769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Kurose, R. Wolf, W. Cerwinka, and D. N. Granger Microvascular Responses to Ischemia/Reperfusion in Normotensive and Hypertensive Rats Hypertension, August 1, 1999; 34(2): 212 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hoshida, N. Yamashita, K. Kawahara, T. Kuzuya, and M. Hori Amelioration by Quinapril of Myocardial Infarction Induced by Coronary Occlusion/Reperfusion in a Rabbit Model of Atherosclerosis : Possible Mechanisms Circulation, January 26, 1999; 99(3): 434 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kurose, R. E. Wolf, M. B. Grisham, and D. N. Granger Hypercholesterolemia Enhances Oxidant Production in Mesenteric Venules Exposed to Ischemia/Reperfusion Arterioscler. Thromb. Vasc. Biol., October 1, 1998; 18(10): 1583 - 1588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hoshida, N. Yamashita, J. Igarashi, K. Aoki, T. Kuzuya, and M. Hori Long-term Probucol Treatment Reverses the Severity of Myocardial Injury in Watanabe Heritable Hyperlipidemic Rabbits Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 2801 - 2807. [Abstract] [Full Text]
|
|