Donate Help Contact The AHA Sign In Home
American Heart Association
Circulation Research
Search: search_blue_button Advanced Search
« Previous Article | Table of Contents | Next Article »
Circulation Research. 1988;62:266-278

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eggermont, J. A.
Right arrow Articles by Casteels, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eggermont, J. A.
Right arrow Articles by Casteels, R.

Circulation Research, Vol 62, 266-278, Copyright © 1988 by American Heart Association

ARTICLES

Ca2+-transport ATPases of vascular smooth muscle

JA Eggermont, M Vrolix, L Raeymaekers, F Wuytack and R Casteels
Laboratorium voor Fysiologie, Universiteit Leuven, Belgium.

To characterize the Ca2+-transport properties of the plasma membrane and of the endoplasmic reticulum of bovine pulmonary artery, membrane vesicles are subfractionated by a procedure of density-gradient centrifugation that takes advantage of the selective effect of digitonin on the density of plasma-membrane vesicles. The obtained endoplasmic-reticulum fraction contains hardly any plasma-membrane vesicles, whereas the plasma-membrane fraction is still contaminated by a substantial amount of endoplasmic-reticulum vesicles. An adenosine 5'- triphosphate (ATP) energized Ca2+-transport system and a Ca2+- stimulated ATPase activity are present in both subcellular fractions. The Ca2+ transport by the plasma membrane is catalyzed by a (Ca2+,Mg2+)- ATPase of Mr 130,000. It binds calmodulin and it has a low steady-state phosphoprotein intermediate level. The endoplasmic-reticulum vesicles contain a Ca2+-transport ATPase of Mr 100,000 that is characterized by a high steady-state phosphointermediate level. It is antigenically related to the Ca2+-pump protein of cardiac sarcoplasmic reticulum. Phospholamban, the regulatory protein of the Ca2+-transport enzyme of cardiac sarcoplasmic reticulum, is also present in the endoplasmic reticulum of the pulmonary artery. A comparison of these fractions with the previously characterized fractions from porcine gastric smooth muscle reveals important differences in the basal Mg2-ATPase activity, in the ratio of the (Ca2+,Mg2+)-ATPase of the plasmalemma to that of the endoplasmic reticulum, and in the ratio of the (Na+,K+)-ATPase activity to the plasmalemmal (Ca2+,Mg2+)-ATPase activity. These differences can be ascribed in part to the species and in part to the tissue. These data suggest that in the bovine pulmonary artery the Ca2+ extrusion via the ATP-dependent Ca2+ pump may have a less predominant role, and that the Ca2+ uptake by the endoplasmic reticulum, and possibly also the Ca2+ extrusion via the Na+-Ca2+ exchanger could be more important in this tissue than in the porcine stomach.


This article has been cited by other articles:


Home page
 Biophys. JHome page
M. A. Machingal and S. V. Ramanan
A Steady-State Electrochemical Model of Vascular Smooth Muscle Cells
Biophys. J., September 1, 2006; 91(5): 1648 - 1662.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. K. Shinjo, I. L. S. Tersariol, V. Oliveira, C. R. Nakaie, M. E. M. Oshiro, A. T. Ferreira, I. A. Santos, C. P. Dietrich, and H. B. Nader
Heparin and Heparan Sulfate Disaccharides Bind to the Exchanger Inhibitor Peptide Region of Na+/Ca2+ Exchanger and Reduce the Cytosolic Calcium of Smooth Muscle Cell Lines. REQUIREMENT OF C4-C5 UNSATURATION AND 1 right-arrow 4 GLYCOSIDIC LINKAGE FOR ACTIVITY
J. Biol. Chem., December 6, 2002; 277(50): 48227 - 48233.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
K. E. PURDY and W. J. ARENDSHORST
Iloprost Inhibits Inositol-1,4,5-Trisphosphate-Mediated Calcium Mobilization Stimulated by Angiotensin II in Cultured Preglomerular Vascular Smooth Muscle Cells
J. Am. Soc. Nephrol., January 1, 2001; 12(1): 19 - 28.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. Michea, V. Valenzuela, I. Bravo, A. Schuster, and E. T. Marusic
Adrenal-dependent modulation of the catalytic subunit isoforms of the Na+-K+-ATPase in aorta
Am J Physiol Endocrinol Metab, December 1, 1998; 275(6): E1072 - E1081.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
H. K. B. SIMMERMAN and L. R. JONES
Phospholamban: Protein Structure, Mechanism of Action, and Role in Cardiac Function
Physiol Rev, October 1, 1998; 78(4): 921 - 947.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
H. KURIYAMA, K. KITAMURA, T. ITOH, and R. INOUE
Physiological Features of Visceral Smooth Muscle Cells, With Special Reference to Receptors and Ion Channels
Physiol Rev, July 1, 1998; 78(3): 811 - 920.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. A. Nazer and C. Van Breemen
A role for the sarcoplasmic reticulum in Ca2+ extrusion from rabbit inferior vena cava smooth muscle
Am J Physiol Heart Circ Physiol, January 1, 1998; 274(1): H123 - H131.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
P. Becker, J. Singer, J. Walsh Jr, and F. Fay
Regulation of calcium concentration in voltage-clamped smooth muscle cells
Science, April 14, 1989; 244(4901): 211 - 214.
[Abstract] [PDF]


Circulation Research Home | Subscriptions | Archives | Feedback | Authors | Help | AHA Journals Home | Search
Copyright © 1988 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited.