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(Circulation. 2009;120:1893-1902.)
© 2009 American Heart Association, Inc.

Molecular Cardiology

Hyperhomocysteinemia Promotes Inflammatory Monocyte Generation and Accelerates Atherosclerosis in Transgenic Cystathionine β-Synthase–Deficient Mice

Daqing Zhang, MD, PhD; Xiaohua Jiang, MS; Pu Fang, BS; Yan Yan, MD; Jian Song, MD, PhD; Sapna Gupta, PhD; Andrew I. Schafer, MD; William Durante, PhD; Warren D. Kruger, PhD; Xiaofeng Yang, MD, PhD; Hong Wang, MD, PhD

From the Department of Pharmacology, Cardiovascular Research Center, and Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pa (D.Z., X.J., P.F., Y.Y., J.S., X.Y., H.W.); Department of Cardiology, Shengjing Hospital of China Medical University, Shenyang, People’s Republic of China (D.Z.); Division of Population Science, Fox Chase Cancer Center, Philadelphia, Pa (S.G, W.D.K.); Department of Medicine, Weill Cornell Medical College, New York, NY (A.I.S.); and Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, Mo (W.D.).

Reprint requests to Dr Hong Wang, Department of Pharmacology and Cardiovascular Research Center, Temple University School of Medicine, 3420 N Broad St, Philadelphia, PA 19140. E-mail hongw{at}temple.edu

Received October 16, 2008; accepted August 27, 2009.

Background— Hyperhomocysteinemia (HHcy) is an independent risk factor for cardiovascular disease. Monocytes display inflammatory and resident subsets and commit to specific functions in atherogenesis. In this study, we examined the hypothesis that HHcy modulates monocyte heterogeneity and leads to atherosclerosis.

Methods and Results— We established a novel atherosclerosis-susceptible mouse model with both severe HHcy and hypercholesterolemia in which the mouse cystathionine β-synthase (CBS) and apolipoprotein E (apoE) genes are deficient and an inducible human CBS transgene is introduced to circumvent the neonatal lethality of the CBS deficiency (Tg-hCBS apoE^–/– Cbs^–/– mice). Severe HHcy accelerated atherosclerosis and inflammatory monocyte/macrophage accumulation in lesions and increased plasma tumor necrosis factor- and monocyte chemoattractant protein-1 levels in Tg-hCBS apoE^–/– Cbs^–/– mice fed a high-fat diet. Furthermore, we characterized monocyte heterogeneity in Tg-hCBS apoE^–/– Cbs^–/– mice and another severe HHcy mouse model (Tg-S466L Cbs^–/–) with a disease-relevant mutation (Tg-S466L) that lacks hyperlipidemia. HHcy increased monocyte population and selective expansion of inflammatory Ly-6C^hi and Ly-6C^mid monocyte subsets in blood, spleen, and bone marrow of Tg-S466L Cbs^–/– and Tg-hCBS apoE^–/– Cbs^–/– mice. These changes were exacerbated in Tg-S466L Cbs^–/– mice with aging. Addition of L-homocysteine (100 to 500 µmol/L), but not L-cysteine, maintained the Ly-6C^hi subset and induced the Ly-6C^mid subset in cultured mouse primary splenocytes. Homocysteine-induced differentiation of the Ly-6C^mid subset was prevented by catalase plus superoxide dismutase and the NAD(P)H oxidase inhibitor apocynin.

Conclusion— HHcy promotes differentiation of inflammatory monocyte subsets and their accumulation in atherosclerotic lesions via NAD(P)H oxidase–mediated oxidant stress.

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Clinical Summaries
Circulation 2009 120: 1843-1844. [Extract] [Full Text]