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(Circulation. 2007;115:2323-2330.)
© 2007 American Heart Association, Inc.

Stroke

Targeting Platelets in Acute Experimental Stroke

Impact of Glycoprotein Ib, VI, and IIb/IIIa Blockade on Infarct Size, Functional Outcome, and Intracranial Bleeding

Christoph Kleinschnitz, MD^*; Miroslava Pozgajova, PhD; Mirko Pham, MD; Martin Bendszus, MD; Bernhard Nieswandt, PhD^*; Guido Stoll, MD^*

From the Departments of Neurology (C.K., G.S.), Clinical Biochemistry and Pathophysiology (M. Pozgajova, B.N.), and Neuroradiology (M. Pham, M.B.) and the Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine (M. Pozgajova, B.N.), University of Würzburg, Würzburg, Germany.

Correspondence to Guido Stoll, MD, Department of Neurology, Bayerische Julius-Maximilians-Universität Würzburg, Josef-Schneider-Str 11, D-97080 Würzburg, Germany. E-mail stoll_g{at}klinik.uni-wuerzburg.de

Received January 18, 2007; accepted March 9, 2007.

Background— Ischemic stroke is a frequent and serious disease with limited treatment options. Platelets can adhere to hypoxic cerebral endothelial cells by binding of their glycoprotein (GP) Ib receptor to von Willebrand factor. Exposure of subendothelial matrix proteins further facilitates firm attachment of platelets to the vessel wall by binding of collagen to their GPVI receptor. In the present study, we addressed the pathogenic role of GPIb, GPVI, and the aggregation receptor GPIIb/IIIa in experimental stroke in mice.

Methods and Results— Complete blockade of GPIb was achieved by intravenous injection of 100 µg Fab fragments of the monoclonal antibody p0p/B to mice undergoing 1 hour of transient middle cerebral artery occlusion. At 24 hours after transient middle cerebral artery occlusion, cerebral infarct volumes were assessed by 2,3,5-triphenyltetrazolium chloride staining. In mice treated with anti-GPIb Fab 1 hour before middle cerebral artery occlusion, ischemic lesions were reduced to 40% compared with controls (28.5±12.7 versus 73.9±17.4 mm³, respectively; P<0.001). Application of anti-GPIb Fab 1 hour after middle cerebral artery occlusion likewise reduced brain infarct volumes (24.5±7.7 mm³; P<0.001) and improved the neurological status. Similarly, depletion of GPVI significantly diminished the infarct volume but to a lesser extent (49.4±19.1 mm³; P<0.05). Importantly, the disruption of early steps of platelet activation was not accompanied by an increase in bleeding complications as revealed by serial magnetic resonance imaging. In contrast, blockade of the final common pathway of platelet aggregation with anti-GPIIb/IIIa F(ab)₂ fragments had no positive effect on stroke size and functional outcome but increased the incidence of intracerebral hemorrhage and mortality after transient middle cerebral artery occlusion in a dose-dependent manner.

Conclusions— Our data indicate that the selective blockade of key signaling pathways of platelet adhesion and aggregation has a different impact on stroke outcome and bleeding complications. Inhibition of early steps of platelet adhesion to the ischemic endothelium and the subendothelial matrix may offer a novel and safe treatment strategy in acute stroke.

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