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(Circulation. 2006;114:886-893.)
© 2006 American Heart Association, Inc.

Coronary Heart Disease

Expression of Heat Shock Protein 27 in Human Atherosclerotic Plaques and Increased Plasma Level of Heat Shock Protein 27 in Patients With Acute Coronary Syndrome

Haing Kee Park, BS^*; Eui-Chul Park, MS^*; Sung Won Bae, PhD^*; Mi Young Park, MS; Seon Woon Kim, MD; Hwan Soo Yoo, PhD; Munkhtsetseg Tudev, BSc; Young Hye Ko, MD; Yoon-Ho Choi, MD; Sungjoo Kim, MD; Dong-Ik Kim, MD; Young Wook Kim, MD; Byung Boong Lee, MD; Jong-Bok Yoon, PhD; Jeong Euy Park, MD

From the Division of Cardiology (H.K.P., S.W.B., M.Y.P, S.W.K, Y.-H.C., J.E.P.), Division of Pathology (Y.H.K.), and Division of Vascular Surgery (S.K., D.-I.K., Y.W.K., B.B.L.), Samsung Medical Center and Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, South Korea; College of Pharmacy, Chungbuk National University, Chongju, South Korea (H.S.Y., M.T.); and Department of Biochemistry and Protein Network Research Center, Yonsei University, Seoul, Korea (E.-C.P., J.-B.Y.).

Correspondence to Jeong Euy Park, MD, Division of Cardiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, South Korea (e-mail jepark{at}smc.samsung.co.kr); or Jong-Bok Yoon, PhD, Department of Biochemistry and Protein Network Research Center, Yonsei University, Seoul 120-749, Korea (e-mail yoonj@yonsei.ac.kr).

Received May 29, 2004; de novo received February 6, 2005; revision received May 21, 2006; accepted June 19, 2006.

Background— We intended to identify proteins that are differentially expressed in human atherosclerotic plaques.

Methods and Results— Comparative 2-dimensional electrophoretic analysis on carotid atherosclerotic endarterectomy specimens (n=10) revealed that heat shock protein 27 (Hsp27) expression was significantly increased in the nearby normal-appearing area compared with the plaque core area from the same vessel specimen, which was further confirmed by Western blot analysis. The Hsp27 expression in the adjacent normal-appearing vessel areas was much higher than that in nonatherosclerotic reference arteries. The phosphorylation of Hsp27 showed a gradation in the degree of phosphorylation: greatest in the reference arteries, intermediate in the adjacent normal-appearing area, and lowest in plaque core area. Immunohistochemical analysis showed that the phosphorylation of Hsp27 of smooth muscle cells in the carotid endarterectomy specimens was decreased compared with that in the reference artery specimen. The mean plasma level of Hsp27 was significantly higher in patients with acute coronary syndrome (ACS) (n=27; 106.1±74.1 ng/mL) than in the normal reference subjects (n=29; 45.8±29.5 ng/mL; P<0.005). The plasma levels of Hsp27 were significantly correlated with those of heat shock protein 70 (Hsp70) (r=0.422, P<0.0005), with adjustment for ACS/reference status.

Conclusions— In the atherosclerotic lesion, Hsp27 expression is increased in the normal-appearing vessel adjacent to atherosclerotic plaque, whereas levels in the plaque itself are significantly decreased. Both plaque and adjacent artery show decreased Hsp27 phosphorylation compared with reference vessel. In ACS, plasma Hsp27 and Hsp70 are increased, and levels of Hsp27 correlate with Hsp70, C-reactive protein, and CD40L levels.

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