Published online before print February 13, 2006, doi: 10.1161/CIRCULATIONAHA.105.588954
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(Circulation. 2006;113:1005-1014.)
© 2006 American Heart Association, Inc.
Molecular Cardiology |
In Vivo Visualization of Embryonic Stem Cell Survival, Proliferation, and Migration After Cardiac Delivery
From the Department of Radiology and Bio-X Program (F.C., S.L., X.X., P.R., M.P., X.Z., X.C., S.S.G., J.C.W.), the Department of Pathology and Developmental Biology (M.D., S.J.D., A.J.C., I.L.W.), the Department of Bioengineering (S.S.G.), and the Department of Medicine, Division of Cardiology (J.C.W.), Stanford University School of Medicine, Stanford, Calif.
Correspondence to Joseph C. Wu, MD, PhD, Stanford University School of Medicine, Edwards Bldg R354, Stanford, CA 94305-5344. E-mail joewu{at}stanford.edu
Received September 14, 2005; revision received November 17, 2005; accepted December 16, 2005.
Background— Recent studies have shown that stem cell therapy can promote tissue regeneration; however, monitoring stem cells in vivo remains problematic owing to limitations of conventional histological assays and imaging modalities.
Methods and Results— Murine embryonic stem (ES) cells were stably transduced with a lentiviral vector carrying a novel triple-fusion (TF) reporter gene that consists of firefly luciferase, monomeric red fluorescence protein, and truncated thymidine kinase (fluc-mrfp-ttk). ES cell viability, proliferation, and differentiation ability were not adversely affected by either reporter genes or reporter probes compared with nontransduced control cells (P=NS). Afterward, 1x107 of ES cells carrying the TF reporter gene (ES-TF) were injected into the myocardium of adult nude rats (n=20). Control animals received nontransduced ES cells (n=6). At day 4, the bioluminescence and positron emission tomography signals in study animals were 3.7x107±5.8x106 photons · s–1 · cm–2 per steradian (sr) and 0.08±0.03% injected dose/g, respectively (P<0.05 versus control). Both signals increased progressively from week 1 to week 4, which indicated ES cell survival and proliferation in the host. Histological analysis demonstrated the formation of intracardiac and extracardiac teratomas. Finally, animals (n=4) that were treated with intraperitoneal injection of ganciclovir (50 mg/kg) did not develop teratomas when compared with control animals (n=4) treated with saline (1 mL/kg).
Conclusion— This is the first study to characterize ES cells that stably express fluorescence, bioluminescence, and positron emission tomography reporter genes and monitor the kinetics of ES cell survival, proliferation, and migration. This versatile imaging platform should have broad applications for basic research and clinical studies on stem cell therapy.
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