This Article Full Text Full Text (PDF) Data Supplement All Versions of this Article: 112/12/1780    most recent CIRCULATIONAHA.105.534008v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Su, L. T. Articles by Stedman, H. H. Search for Related Content PubMed PubMed Citation Articles by Su, L. T. Articles by Stedman, H. H. Related Collections Gene therapy Endothelium/vascular type/nitric oxide Other Vascular biology

(Circulation. 2005;112:1780-1788.)
© 2005 American Heart Association, Inc.

Molecular Medicine

Uniform Scale-Independent Gene Transfer to Striated Muscle After Transvenular Extravasation of Vector

Leonard T. Su, MD^*; Kapil Gopal, MD^*; Zhonglin Wang, MD; Xiaoqing Yin, MD; Anthony Nelson, BS; Benjamin W. Kozyak, BS; James M. Burkman, MD; Marilyn A. Mitchell, BS; David W. Low, MD; Charles R. Bridges, MD, ScD; Hansell H. Stedman, MD

From the Department of Surgery, University of Pennsylvania, Philadelphia (L.T.S., K.G., Z.W., X.Y., A.N., B.W.K., M.A.M., D.W.L., C.R.B., H.H.S.); Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, (H.H.S.); and Department of Anesthesiology, University of Washington, Seattle (J.M.B.).

Correspondence to Hansell H. Stedman, MD, Department of Surgery, University of Pennsylvania, 608 BRB II/III, 421 Curie Blvd, Philadelphia, PA 19104. E-mail hstedman{at}mail.med.upenn.edu

Received January 5, 2005; revision received June 15, 2005; accepted June 17, 2005.

Background— The muscular dystrophies exemplify a class of systemic disorders for which widespread protein replacement in situ is essential for treatment of the underlying genetic disorder. Somatic gene therapy will require efficient, scale-independent transport of DNA-containing macromolecular complexes too large to cross the continuous endothelia under physiological conditions. Previous studies in large-animal models have revealed a trade-off between the efficiency of gene transfer and the inherent safety of the required surgical and pharmacological interventions to achieve this.

Methods and Results— Rats and dogs underwent limb or hemibody isolation via atraumatic tourniquet placement or myocardial isolation via heterotopic transplantation. Recombinant adenovirus (10¹³ particles per kilogram) or recombinant adeno-associated virus (10¹⁴ genome copies/kg) encoding the lacZ transgene was delivered through pressurized venous infusion without pharmacological mediators. Muscle exhibited almost 100% myofiber transduction in rats and dogs by X-galactosidase staining and significantly higher ß-galactosidase levels compared with nonpressurized delivery. No significant difference was seen in ß-galactosidase levels between 100- or 400-mm Hg groups. The <50-mm Hg group yielded inhomogeneous and significantly lower transgene expression.

Conclusions— Uniform scale- and vector-independent skeletal and cardiac myofiber transduction is facilitated by pressurized venous infusion in anatomic domains isolated from the central circulation without pharmacological interference with cardiovascular homeostasis. We provide the first demonstration of uniform gene transfer to muscle fibers of an entire extremity in the dog, providing a firm foundation for further translational studies of efficacy in canine models for human diseases.

---

 

CLINICAL PERSPECTIVE