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(Circulation. 2004;109:1763-1768.)
© 2004 American Heart Association, Inc.

Basic Science Reports

Thermal Treatment Attenuates Neointimal Thickening With Enhanced Expression of Heat-Shock Protein 72 and Suppression of Oxidative Stress

Motoi Okada, MD; Naoyuki Hasebe, MD, PhD; Yoshiaki Aizawa, MD, PhD; Kazuma Izawa, MD; Jun-ichi Kawabe, MD, PhD; Kenjiro Kikuchi, MD, PhD

From the First Department of Internal Medicine, Asahikawa Medical College, Asahikawa, Japan.

Correspondence to Naoyuki Hasebe, MD, First Department of Medicine, Asahikawa Medical College, 2-1-1-1, Midorigaoka-Higashi, Asahikawa 078-8510, Japan. E-mail haselove{at}asahikawa-med.ac.jp

Received May 12, 2003; de novo received October 5, 2003; accepted January 5, 2004.

Background— The beneficial effects of thermal therapy have been reported in several cardiovascular diseases. However, it is unknown whether the thermal treatment has some beneficial roles against the development of atherosclerosis.

Methods and Results— The inflammatory arterial lesion was introduced by placement of a polyethylene cuff on femoral arteries of male Sprague-Dawley rats for 4 weeks. Thermal-treated group underwent daily bathing in 41°C hot water for 15 minutes. Neointimal thickening along with immunohistochemical expression of heat-shock proteins (HSPs), monocyte chemoattractant protein-1 (MCP-1), and NADPH oxidase were compared with those of a thermally untreated (Control) group. Morphometric analysis demonstrated a significant suppression of neointimal thickening in thermal-treated group compared with the Control group (intimal/medial area ratios, 0.01±0.01 versus 0.31±0.04, P<0.01). Expression of MCP-1 and infiltration of ED-positive cells were enhanced in the adventitial layer of Control. More importantly, expression of HSP72 in media was enhanced by thermal treatment. Expression of p22-phox, the major membrane subunit of NADPH oxidase, and MCP-1 was augmented in cuff-injured adventitia of the Control but not the thermal-treated groups.

Conclusions— Thermal treatment significantly attenuated infiltration of inflammatory cells in adventitia and suppressed neointimal thickening in cuff-injured arteries with the enhancement of HSP72 expression and suppression of oxidative stress.

Key Words: heat-shock proteins • atherosclerosis • oxidative stress

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