Published online before print September 8, 2003, doi: 10.1161/01.CIR.0000091083.61609.DF
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(Circulation. 2003;108:1575.)
© 2003 American Heart Association, Inc.
Clinical Investigation and Reports |
Estrogen Stimulates Dimethylarginine Dimethylaminohydrolase Activity and the Metabolism of Asymmetric Dimethylarginine
From the Department of Obstetrics and Gynaecology (D.P.H.), Royal Sussex County Hospital, Eastern Road, Brighton, and Departments of Biochemistry and Immunology (J.E.C., G.S.J.W.) and Oncology, Gastroenterology, Endocrinology, and Medicine (S.S.N.), St George’s Hospital Medical School, London, UK.
Correspondence to Guy Whitley, Department of Biochemistry and Immunology, St George’s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK. E-mail g.whitley{at}sghms.ac.uk
Received March 31, 2003; de novo received June 5, 2003; revision received July 16, 2003; accepted July 17, 2003.
Background— Experimental evidence suggests that estrogens stimulate the production of nitric oxide (NO) by vascular endothelial cells. This effect has been attributed to increased expression and enzymatic activity of both the constitutive and inducible isoforms of NO synthase. In this study, we have investigated whether estrogens regulate the metabolism or release of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of NO synthase.
Methods and Results— The concentration of ADMA in the plasma of 15 postmenopausal women was 0.722±0.04 µmol/L (mean±SEM). Two weeks after subcutaneous implantation with estradiol, there was an increase in plasma estradiol concentration from 0.693±0.075 to 0.81±87 nmol/L, which was accompanied by a significant fall in plasma ADMA concentration to 0.588±0.03 µmol/L (P=0.006). Human and murine endothelial cell lines previously cultured in estrogen-free medium and then exposed to 17ß-estradiol showed a dose-dependent decrease in the release of ADMA. This reached statistical significance at 10-14 mol/L 17ß-estradiol and was accompanied by a corresponding increase in the activity of dimethylarginine dimethylaminohydrolase (DDAH), an enzyme that catalyzes the metabolism of ADMA.
Conclusions— We have demonstrated that estrogens can alter the catabolism and release of ADMA in vitro and reduce the circulating concentration in vivo. We therefore propose that increased DDAH activity and the subsequent fall in ADMA could contribute to the positive effect of estrogen on NO synthesis.
Key Words: endothelium • nitric oxide • cardiovascular diseases • asymmetric dimethylarginine
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