Published online before print May 12, 2003, doi: 10.1161/01.CIR.0000068316.53218.49
(Circulation. 2003;107:2459.)
© 2003 American Heart Association, Inc.
Clinical Investigation and Reports |
ß-Blockers Restore Calcium Release Channel Function and Improve Cardiac Muscle Performance in Human Heart Failure
From the Center for Molecular Cardiology (S.R., X.H.T.W., J.A.V., D.M., D.B., A.R.M.); Circulatory Physiology and Cardiology Divisions, Department of Medicine (J.A.V., D.M., D.B.); Department of Pharmacology (A.R.M.); and Department of Surgery (A.B.), Columbia University College of Physicians and Surgeons, New York, NY.
Correspondence to Andrew R. Marks, Center for Molecular Cardiology, Box 65, Columbia University College of Physicians and Surgeons, Room 9-401, 630 West 168th St, New York, NY 10032. E-mail arm42{at}columbia.edu
Background— Chronic ß-adrenergic receptor (ß-AR) blockade improves cardiac contractility and prolongs survival in patients with heart failure; however, the mechanisms underlying these favorable responses are poorly understood. Stress-induced activation of the sympathetic nervous system results in protein kinase A (PKA)-mediated phosphorylation of the calcium (Ca2+) release channel/cardiac ryanodine receptor (RyR2), required for cardiac excitation-contraction (EC) coupling, activating the RyR2 channel, and increasing cardiac contractility. The hyperadrenergic state of heart failure results in leaky RyR2 channels attributable to PKA hyperphosphorylation and depletion of the stabilizing FK506 binding protein, FKBP12.6. We tested the hypothesis that improved cardiac muscle function attributable to ß-AR blockade is associated with restoration of normal RyR2 channel function in patients with heart failure.
Methods and Results— We assessed the effects of ß-AR blockade on left ventricular volume using isolated perfused hearts and ß-agonist responsiveness using muscle strips from patients undergoing transplantation. Twenty-four human hearts were examined, 10 from patients with heart failure treated with ß-AR blockers (carvedilol, metoprolol, or atenolol), 9 from patients with heart failure without ß-AR blocker treatment, and 5 normal hearts. RyR2 PKA phosphorylation was determined by back-phosphorylation, FKBP12.6 in the RyR2 macromolecular complex was determined by coimmunoprecipitation, and channel function was assayed using planar lipid bilayers. ß-AR blockers reduced left ventricular volume (reverse remodeling) and restored ß-agonist response in cardiac muscle from patients with heart failure. Improved cardiac muscle function was associated with restoration of normal FKBP12.6 levels in the RyR2 macromolecular complex and RyR2 channel function.
Conclusions— Improved cardiac muscle function during ß-AR blockade is associated with improved cardiac Ca2+ release channel function in patients with heart failure.
Key Words: heart failure • calcium • ion channels • remodeling • catecholamines
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