Published online before print April 21, 2003, doi: 10.1161/01.CIR.0000070082.64414.B5
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(Circulation. 2003;107:2078.)
© 2003 American Heart Association, Inc.
Brief Rapid Communications |
Isolation of Bone Marrow Stromal Cell–Derived Smooth Muscle Cells by a Human SM22
Promoter
In Vitro Differentiation of Putative Smooth Muscle Progenitor Cells of Bone Marrow
From the Departments of Cardiology (Y. Kashiwakura, Y. Katoh, H.K., N.T., H.D.), Hematology (K.T., K.S.), Gynecology (S.Y.), and Neurology (M.Y.), Juntendo University School of Medicine, Tokyo, Japan.
Correspondence to Youichi Katoh, MD, PhD, Department of Cardiology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan. E-mail Katoyo{at}med.juntendo.ac.jp
Background— Bone marrow stromal cells (BMSCs) have many characteristics of mesenchymal stem cells that can differentiate into smooth muscle cells (SMCs). However, there have been few studies closely following the cell development of smooth muscle lineage among BMSCs.
Methods and Results— To investigate the possible existence of a cell population committed to the SMC lineage among bone marrow adhesion cells, we tried to detect and follow the in vitro differentiation of such a cell type by using a promoter-sorting method with a human SM22
promoter (-480 bp)/green fluorescent protein (GFP) construct. The construct was transfected to adhesion cells that appeared 5 days after the seeding of mononuclear cells from bone marrow. GFP was first detectable 5 days after the transfection in a cell population [Ad(G) cells], which expressed PDGF-ß but neither mature (calponin) nor immature (SMemb) SMC-specific proteins at that time. However, the cells were eventually grown into individual clones that expressed SMC-specific proteins (-smooth muscle actin, calponin, and SM-1), suggesting that Ad(G) cells have partly at least progenitor properties. Because early studies have reported that PDGF-ß signaling plays pivotal roles in the differentiation of mesenchymal smooth muscle progenitor cells, Ad(G) cells might be putative mesenchymal smooth muscle progenitors expressing PDGF-ß.
Conclusions— We demonstrated the presence of a cell population fated to become SMCs and followed their differentiation into SMCs among BMSCs.
Key Words: bone marrow • stromal cells • stem cells • cell differentiation • muscle, smooth
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