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(Circulation. 2002;105:490.)
© 2002 American Heart Association, Inc.

Basic Science Reports

Augmented Expression of Neuronal Nitric Oxide Synthase in the Atria Parasympathetically Decreases Heart Rate During Acute Myocardial Infarction in Rats

Yoshihito Takimoto, MD; Takeshi Aoyama, MD, PhD; Koichi Tanaka, PhD; Reiko Keyamura, MD; Yoshiki Yui, MD, PhD; Shigetake Sasayama, MD, PhD

From the Department of Cardiovascular Medicine (Y.T., T.A., R.K., Y.Y., S.S.), Graduate School of Medicine, Kyoto University, Kyoto, Japan, and Department of Anatomy (K.T.), Hyogo College of Medicine, Hyogo, Japan.

Correspondence to Takeshi Aoyama, MD, Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawaracho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. E-mail taoyama{at}kuhp.kyoto-u.ac.jp

Background— Nitric oxide (NO) synthesized within sinoatrial cells recently has been shown to participate in the autonomic control of heart rate. We hypothesized that NO in the neuronal cells in the heart was increased and parasympathetically regulated heart rate after myocardial infarction (MI).

Methods and Results— We examined heart rate dynamics and neuronal NO synthase (nNOS) expression and activities in the atria of rats with MI 1, 3, 7, and 14 days after MI (n=7 to 22 for each group). Both the mRNA levels of nNOS in the atria determined by competitive reverse transcriptase-polymerase chain reaction and the protein levels determined by Western blotting were significantly increased compared with controls 1, 3, and 7 days after MI. nNOS activity in the atria 1 day after infarction was also increased in MI rats. nNOS immunoreactivity was observed in nerve fibers in the atria. After infusion of a specific inhibitor of nNOS and iNOS, 1-(2-trifluoromethylphenyl) imidazole (TRIM) (50 mg/kg IV), heart rate was significantly (P<0.01) increased in MI rats compared with controls 1, 3, and 7 days after MI. The iNOS-specific inhibitor, 1400W (10 mg/kg SC), did not significantly affect the heart rate in rats with MI. The effect of TRIM was abolished by pretreatment with L-arginine (25 mg/kg IV) or by parasympathetic blockade with atropine but not by propranolol. There was a strong correlation (r=0.837, P<0.0001) between the nNOS protein expression and heart rate change after TRIM infusion.

Conclusions— These results indicate that increased nNOS parasympathetically decreased heart rate via the production of NO in rats with acute MI.

Key Words: nitric oxide synthase • heart rate • myocardial infarction

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