Published online before print April 22, 2002, doi: 10.1161/01.CIR.0000015604.88808.74
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(Circulation. 2002;105:2303.)
© 2002 American Heart Association, Inc.
Basic Science Reports |
Cardiac Membrane Fatty Acid Composition Modulates Myocardial Oxygen Consumption and Postischemic Recovery of Contractile Function
From the Cardiac Surgical Research Unit, Alfred Hospital and Baker Medical Research Institute, Monash University Faculty of Medicine (S.P.), Melbourne, Australia; and Smart Foods Centre, Department of Biomedical Science, University of Wollongong (P.L.M.), New South Wales, Australia.
Correspondence to Associate Professor Peter L. McLennan, Department of Biomedical Science, University of Wollongong, NSW 2522, Australia. E-mail petermcl{at}uow.edu.au
Background— Regular fish consumption is associated with low cardiovascular disease morbidity and mortality. Fish oils modify cardiac membrane phospholipid fatty acid composition with potent antiarrhythmic effects. We tested the effects of dietary fish oil on ventricular hemodynamics and myocardial oxygen consumption (MVO2).
Methods and Results— Male Wistar rats were fed for 16 weeks on a reference diet rich in n-6 polyunsaturated fatty acids (PUFA), a diet rich in saturated animal fat (SAT), or a diet rich in n-3 PUFA from fish oil. Isolated working hearts were perfused with porcine erythrocytes (40% hematocrit) at 75 mm Hg afterload with variable preload (5 to 20 mm Hg) or with low coronary flow ischemia with maintained afterload, preload, and heart rate, then reperfused. MVO2 was low and coronary perfusion reserve high in n-3 PUFA hearts, and cardiac output increased with workload. The n-3 PUFA reduced ischemic markers—acidosis, K+, lactate, and creatine kinase—and increased contractile recovery during reperfusion. SAT hearts had high MVO2, low coronary perfusion reserve, and poor contractile function and recovery. Dietary differences in MVO2 were abolished by KCl arrest (basal metabolism) or ruthenium red (3.4 µmol/L) but not by ryanodine (1 nmol/L). Fish oil or ryanodine, but not ruthenium red, prevented ventricular fibrillation in reperfusion.
Conclusions— Dietary fish oil directly influenced heart function and improved cardiac responses to ischemia and reperfusion. The n-3 PUFA reduced oxygen consumption at any given work output and increased postischemic recovery. Thus, direct effects on myocardial function may contribute to the altered cardiovascular disease profile associated with fish consumption.
Key Words: myocardium • oxygen • ischemia • fatty acids • fish oils
This article has been cited by other articles:
 |
|
 |
|
  M. K. Duda, K. M. O'Shea, and W. C. Stanley {omega}-3 polyunsaturated fatty acid supplementation for the treatment of heart failure: mechanisms and clinical potential Cardiovasc Res, October 1, 2009; 84(1): 33 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Shah, M. K. Duda, K. M. O'Shea, G. C. Sparagna, D. J. Chess, R. J. Khairallah, I. Robillard-Frayne, W. Xu, R. C. Murphy, C. Des Rosiers, et al. The Cardioprotective Effects of Fish Oil During Pressure Overload Are Blocked by High Fat Intake: Role Of Cardiac Phospholipid Remodeling Hypertension, September 1, 2009; 54(3): 605 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Huggins, C. L. Curl, R. Patel, P. L. McLennan, M. L. Theiss, T. Pedrazzini, S. Pepe, and L. M. D. Delbridge Dietary fish oil is antihypertrophic but does not enhance postischemic myocardial function in female mice Am J Physiol Heart Circ Physiol, April 1, 2009; 296(4): H957 - H966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Duda, K. M. O'Shea, A. Tintinu, W. Xu, R. J. Khairallah, B. R. Barrows, D. J. Chess, A. M. Azimzadeh, W. S. Harris, V. G. Sharov, et al. Fish oil, but not flaxseed oil, decreases inflammation and prevents pressure overload-induced cardiac dysfunction Cardiovasc Res, February 1, 2009; 81(2): 319 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. G. Abdukeyum, A. J. Owen, and P. L. McLennan Dietary (n-3) Long-Chain Polyunsaturated Fatty Acids Inhibit Ischemia and Reperfusion Arrhythmias and Infarction in Rat Heart Not Enhanced by Ischemic Preconditioning J. Nutr., October 1, 2008; 138(10): 1902 - 1909. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Barcelo-Coblijn, E. J Murphy, R. Othman, M. H Moghadasian, T. Kashour, and J. K Friel Flaxseed oil and fish-oil capsule consumption alters human red blood cell n-3 fatty acid composition: a multiple-dosing trial comparing 2 sources of n-3 fatty acid Am. J. Clinical Nutrition, September 1, 2008; 88(3): 801 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Igarashi, K. Ma, L. Chang, J. M. Bell, and S. I. Rapoport Rat heart cannot synthesize docosahexaenoic acid from circulating {alpha}-linolenic acid because it lacks elongase-2 J. Lipid Res., August 1, 2008; 49(8): 1735 - 1745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. Abel, S. E. Litwin, and G. Sweeney Cardiac Remodeling in Obesity Physiol Rev, April 1, 2008; 88(2): 389 - 419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sakabe, A. Shiroshita-Takeshita, A. Maguy, C. Dumesnil, A. Nigam, T.-K. Leung, and S. Nattel Omega-3 Polyunsaturated Fatty Acids Prevent Atrial Fibrillation Associated With Heart Failure but Not Atrial Tachycardia Remodeling Circulation, November 6, 2007; 116(19): 2101 - 2109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pepe and P. L. McLennan (n-3) Long Chain PUFA Dose-Dependently Increase Oxygen Utilization Efficiency and Inhibit Arrhythmias after Saturated Fat Feeding in Rats J. Nutr., November 1, 2007; 137(11): 2377 - 2383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Duda, K. M. O'Shea, B. Lei, B. R. Barrows, A. M. Azimzadeh, T. E. McElfresh, B. D. Hoit, W. J. Kop, and W. C. Stanley Dietary supplementation with {omega}-3 PUFA increases adiponectin and attenuates ventricular remodeling and dysfunction with pressure overload Cardiovasc Res, November 1, 2007; 76(2): 303 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mozaffarian Fish Oil and Prevention of Atrial Fibrillation J. Am. Coll. Cardiol., October 9, 2007; 50(15): 1513 - 1514. [Full Text] [PDF]
|
|
|
|
|
|
B. London, C. Albert, M. E. Anderson, W. R. Giles, D. R. Van Wagoner, E. Balk, G. E. Billman, M. Chung, W. Lands, A. Leaf, et al. Omega-3 Fatty Acids and Cardiac Arrhythmias: Prior Studies and Recommendations for Future Research: A Report from the National Heart, Lung, and Blood Institute and Office of Dietary Supplements Omega-3 Fatty Acids and Their Role in Cardiac Arrhythmogenesis Workshop Circulation, September 4, 2007; 116(10): e320 - e335. [Full Text] [PDF]
|
|
|
|
|
|
N. Sharma, I. C. Okere, M. K. Duda, D. J. Chess, K. M. O'Shea, and W. C. Stanley Potential impact of carbohydrate and fat intake on pathological left ventricular hypertrophy Cardiovasc Res, January 15, 2007; 73(2): 257 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Fiaccavento, F. Carotenuto, M. Minieri, L. Masuelli, A. Vecchini, R. Bei, A. Modesti, L. Binaglia, A. Fusco, A. Bertoli, et al. {alpha}-Linolenic Acid-Enriched Diet Prevents Myocardial Damage and Expands Longevity in Cardiomyopathic Hamsters Am. J. Pathol., December 1, 2006; 169(6): 1913 - 1924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Chatelier, N. Imbert, J. L. Z. Infante, D. J. McKenzie, and P. Bois Effects of oleic acid on the high threshold barium current in seabass Dicentrarchus labrax ventricular myocytes J. Exp. Biol., October 15, 2006; 209(20): 4033 - 4039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chatelier, D. J. McKenzie, A. Prinet, R. Galois, J. Robin, J. Zambonino, and G. Claireaux Associations between tissue fatty acid composition and physiological traits of performance and metabolism in the seabass (Dicentrarchus labrax) J. Exp. Biol., September 1, 2006; 209(17): 3429 - 3439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. McGuinness, T.G. Neilan, A. Sharkasi, D. Bouchier-Hayes, and J.M. Redmond Myocardial protection using an omega-3 fatty acid infusion: Quantification and mechanism of action J. Thorac. Cardiovasc. Surg., July 1, 2006; 132(1): 72 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. C. Stanley, F. A. Recchia, and I. C. Okere Metabolic therapies for heart disease: Fish for prevention and treatment of cardiac failure? Cardiovasc Res, November 1, 2005; 68(2): 175 - 177. [Full Text] [PDF]
|
|
|
|
|
|
D. Mozaffarian, C. L. Bryson, R. N. Lemaitre, G. L. Burke, and D. S. Siscovick Fish Intake and Risk of Incident Heart Failure J. Am. Coll. Cardiol., June 21, 2005; 45(12): 2015 - 2021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pepe and E. G. Lakatta Aging hearts and vessels: Masters of adaptation and survival Cardiovasc Res, May 1, 2005; 66(2): 190 - 193. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Tavazzi, G. Tognoni, M. G. Franzosi, R. Latini, A. P. Maggioni, R. Marchioli, G. L. Nicolosi, M. Porcu, and on behalf of the GISSI-HF investigators Rationale and design of the GISSI heart failure trial: a large trial to assess the effects of n-3 polyunsaturated fatty acids and rosuvastatin in symptomatic congestive heart failure Eur J Heart Fail, August 1, 2004; 6(5): 635 - 641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Mozaffarian, B. M. Psaty, E. B. Rimm, R. N. Lemaitre, G. L. Burke, M. F. Lyles, D. Lefkowitz, and D. S. Siscovick Fish Intake and Risk of Incident Atrial Fibrillation Circulation, July 27, 2004; 110(4): 368 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ovide-Bordeaux and A. Grynberg Docosahexaenoic acid affects insulin deficiency- and insulin resistance-induced alterations in cardiac mitochondria Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2004; 286(3): R519 - R527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T Erkkila, S. Lehto, K. Pyorala, and M. I. Uusitupa n-3 Fatty acids and 5-y risks of death and cardiovascular disease events in patients with coronary artery disease Am. J. Clinical Nutrition, July 1, 2003; 78(1): 65 - 71. [Abstract] [Full Text] [PDF]
|
|