doi: 10.1161/hc4501.098952
(Circulation. 2001;104:2447.)
© 2001 American Heart Association, Inc.
Basic Science Reports |
Elevating High-Density Lipoprotein Cholesterol in Apolipoprotein E-Deficient Mice Remodels Advanced Atherosclerotic Lesions by Decreasing Macrophage and Increasing Smooth Muscle Cell Content
From the Department of Medicine and the Zena and Michael A. Wiener Cardiovascular Institute (J.X.R., J.L., E.D.R., R.P.C., V.I.E., J.T.F., E.A.F.) and the Department of Surgery (E.D.R.), Mount Sinai School of Medicine; and the Laboratory of Biochemical Genetics and Metabolism, Rockefeller University (H.M.D., J.L.B.), New York, NY.
Correspondence to Dr Edward A. Fisher, Box 1269, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029. E-mail edward.fisher{at}mssm.edu
Background— HDL cholesterol levels are inversely correlated with coronary heart disease risk in humans, and in animal studies, HDL elevation decreases formation and progression of foam-cell lesions. The potential for HDL to affect preexisting advanced atherosclerotic lesions is not known. To approach this issue, we used a novel mouse aortic transplantation model.
Methods and Results— ApoE-deficient (EKO) mice were fed a Western-type diet for 6 months, and thoracic aortic segments containing advanced lesions replaced segments of the abdominal aorta of 4-month-old EKO syngeneic mice not expressing (plasma HDL cholesterol 
26 mg/dL) or expressing (HDL 64 mg/dL) a human apoAI (hAI) transgene. Both types of recipients had comparable non-HDL cholesterol levels. Five months after transplantation, mice were killed and grafts analyzed. Compared with lesion area in pretransplant mice (0.14±0.04 mm2, mean±SEM), there was progression in the EKO recipients (0.39±0.06 mm2, P<0.01). Compared with EKO recipients, hAI/EKO recipients had retarded progression (0.24±0.04 mm2, P<0.05). Immunostaining for CD68 and other macrophage-associated proteins, monocyte chemoattractant protein-1, acyl coenzyme A:cholesterol acyltransferase, and tissue factor, in lesions of pretransplant and EKO recipient mice showed abundant macrophages. In contrast, compared with any other group, lesional macrophage area in hAI/EKO mice decreased >80% (P<0.003), and smooth muscle cell content (
-actin staining) increased >300% (P<0.006). The decrease in macrophages and increase in smooth muscle cells was primarily in the superficial subendothelial layer.
Conclusions— Increasing HDL cholesterol levels in EKO mice retards progression of advanced atherosclerotic lesions and remodels them to a more stable-appearing phenotype.
Key Words: muscle smooth • remodeling • plaque • lipoproteins
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