This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yoshioka, K. Articles by Dae, M. Search for Related Content PubMed PubMed Citation Articles by Yoshioka, K. Articles by Dae, M. Related Collections Arrhythmias, clinical electrophysiology, drugs Autonomic, reflex, and neurohumoral control of circulation Cardiovascular imaging agents/Techniques Nuclear cardiology and PET Imaging

(Circulation. 2000;101:1060.)
© 2000 American Heart Association, Inc.

Basic Science Reports

Heterogeneous Sympathetic Innervation Influences Local Myocardial Repolarization in Normally Perfused Rabbit Hearts

Koichiro Yoshioka, MD; Dong-Wei Gao, MD; Michael Chin, BS; Carol Stillson, BA; Elizabeth Penades, BS; Michael Lesh, MD; William O’Connell, MS; Michael Dae, MD

From the Cardiovascular Research Institute, University of California, San Francisco.

Correspondence to Michael W. Dae, MD, Box 0252, University of California, San Francisco, CA 94143-0252. E-mail michael.dae{at}radiology.ucsf.edu

Background—Heterogeneity of sympathetic innervation is thought to contribute to the potential for fatal arrhythmia. However, little is known about the effects of heterogeneous innervation on repolarization.

Methods and Results—To assess this relationship, we measured activation recovery intervals (ARIs) from 64 epicardial sites in 11 rabbits studied 2 weeks after regional denervation produced by phenol and 4 sham-operated rabbits. ARI results were compared with the distribution of sympathetic innervation measured from 3D reconstructions of serial autoradiographs of [¹²⁵I]metaiodobenzylguanidine and ^99mTc-sestamibi. ARIs were recorded during baseline sinus rhythm, norepinephrine (NE) infusion (0.1 µg · kg^-1 · min^-1), and left stellate ganglion stimulation (SS). NE shortened ARI in 98% of electrodes in the denervated region. The degree of ARI shortening and dispersion increased (P<0.001 and P<0.01, respectively) as denervation became more severe. SS shortened ARI in 30% of electrodes in the denervated area, with increased shortening and dispersion related to increased severity of denervation (P<0.01). SS prolonged ARI in 70% of electrodes in the denervated area, with no correlation with severity of denervation.

Conclusions—The magnitude and dispersion of local repolarization responses are related to the severity of denervation, as well as the type of stimulation: neural (SS) versus humoral (NE). The differences may relate to the concentration of NE released.

Key Words: myocardium • repolarization • MIBG • nervous system

This article has been cited by other articles:

				M. Amino, K. Yoshioka, T. Tanabe, E. Tanaka, H. Mori, Y. Furusawa, W. Zareba, M. Yamazaki, H. Nakagawa, H. Honjo, et al. Heavy ion radiation up-regulates Cx43 and ameliorates arrhythmogenic substrates in hearts after myocardial infarction Cardiovasc Res, December 1, 2006; 72(3): 412 - 421. [Abstract] [Full Text] [PDF]

				K. Mardon, O. Montagne, N. Elbaz, Z. Malek, A. Syrota, J.-L. Dubois-Rande, M. Meignan, and P. Merlet Uptake-1 Carrier Downregulates in Parallel with the {beta}-Adrenergic Receptor Desensitization in Rat Hearts Chronically Exposed to High Levels of Circulating Norepinephrine: Implications for Cardiac Neuroimaging in Human Cardiomyopathies J. Nucl. Med., September 1, 2003; 44(9): 1459 - 1466. [Abstract] [Full Text] [PDF]

				Y.-B. Liu, C.-C. Wu, L.-S. Lu, M.-J. Su, C.-W. Lin, S.-F. Lin, L. S. Chen, M. C. Fishbein, P.-S. Chen, and Y.-T. Lee Sympathetic Nerve Sprouting, Electrical Remodeling, and Increased Vulnerability to Ventricular Fibrillation in Hypercholesterolemic Rabbits Circ. Res., May 30, 2003; 92(10): 1145 - 1152. [Abstract] [Full Text] [PDF]

				M E Marketou, E N Simantirakis, V K Prassopoulos, S I Chrysostomakis, A A Velidaki, N S Karkavitsas, and P E Vardas Assessment of myocardial adrenergic innervation in patients with sick sinus syndrome: effect of asynchronous ventricular activation from ventricular apical stimulation Heart, September 1, 2002; 88(3): 255 - 259. [Abstract] [Full Text] [PDF]

				E.N Simantirakis, V.K Prassopoulos, S.I Chrysostomakis, G.E Kochiadakis, S.I Koukouraki, J.P Lekakis, N.S Karkavitsas, and P.E Vardas Effects of asynchronous ventricular activation on myocardial adrenergic innervation in patients with permanent dual-chamber pacemakers. An I123-metaiodobenzylguanidine cardiac scintigraphic study Eur. Heart J., February 2, 2001; 22(4): 323 - 332. [Abstract] [PDF]

				G. Bru-Mercier, E. Deroubaix, D. Rousseau, A. Coulombe, and J.-F. Renaud Depressed transient outward potassium current density in catecholamine-depleted rat ventricular myocytes Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1237 - H1247. [Abstract] [Full Text] [PDF]