(Circulation. 2000;101:908.)
© 2000 American Heart Association, Inc.
Basic Science Reports |
Interleukin-10 Inhibits Intimal Hyperplasia After Angioplasty or Stent Implantation in Hypercholesterolemic Rabbits
From U460 INSERM, Faculté Xavier Bichat (L.J.F., L.A., N.N., J.-B.M., P.G.S.); Service de Cardiologie A, Hôpital Bichat (L.J.F., G.S.); Service d’Anatomo-Pathologie, Hôpital Jean Verdier (M.Z.); and Service d’Anatomo-Pathologie, Hôpital Bichat (J.-P.B.); Paris, France.
Correspondence to P. Gabriel Steg, MD, U460 INSERM, Faculté Xavier Bichat, 16, rue Henri Huchard, 75018 Paris, France. E-mail gabriel.steg{at}bch.ap-hop-paris.fr
Background—Intimal hyperplasia after stent implantation is the main cause of in-stent restenosis. Activated monocytes play a key role in intimal growth. The anti-inflammatory cytokine interleukin-10 (IL-10) is a potent monocyte deactivator, endogenously produced in the atherosclerotic plaque. We tested the hypothesis that exogenous IL-10 may limit postangioplasty intimal hyperplasia after balloon angioplasty or stenting.
Methods and Results—Hypercholesterolemic rabbits
were treated with recombinant human IL-10 (rhuIL-10) for 3 days after
balloon angioplasty or 28 days after stent implantation. High IL-10
serum levels and intense deactivation of circulating monocytic cells,
assessed by inhibition of IL-1ß release by
lipopolysaccharide-stimulated whole blood, were detected for at
least 8 hours after rhuIL-10 intravenous injection (ELISA).
Morphometric analyses, performed 28 days after injury,
indicated that rhuIL-10 reduced intimal growth by 
50% after balloon
angioplasty or stenting, resulting in more preserved lumen in stented
arteries. Moreover, rhuIL-10 reduced macrophage infiltration by
67% and proliferative activity by 81% in the intima and the media. No
toxic effect was detected except minor changes in blood cell count.
Conclusions—The anti-inflammatory cytokine rhuIL-10 reduces postinjury intimal hyperplasia. The potent attenuation of in-stent intimal growth by rhuIL-10 and its favorable toxicity profile suggest that rhuIL-10 may be useful in the prevention of in-stent restenosis.
Key Words: interleukins • stents • restenosis
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