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(Circulation. 1999;100:1443-1449.)
© 1999 American Heart Association, Inc.

Basic Science Reports

Mast Cells Cause Apoptosis of Cardiomyocytes and Proliferation of Other Intramyocardial Cells In Vitro

Masatake Hara, MD; Akira Matsumori, MD, PhD; Koh Ono, MD, PhD; Hiroshi Kido, MD, PhD; Myung-Woo Hwang, MD; Tadashi Miyamoto, MD; Atsushi Iwasaki, MD; Masaharu Okada, MD; Kazuki Nakatani, MD, PhD; Shigetake Sasayama, MD, PhD

From the Department of Cardiovascular Medicine, Kyoto University, Kyoto, Japan (M.H., A.M., K.O., M.-W.H., T.M., A.I., M.O.); Division of Enzyme Chemistry, Institute of Enzyme Research, University of Tokushima, Tokushima, Japan (H.K.); and Second Department of Anatomy, Osaka City University Medical School, Osaka, Japan (K.N.).

Correspondence to Akira Matsumori, MD, PhD, Department of Cardiovascular Medicine, Kyoto University, 54 Kawaracho Shogoin, Sakyo-ku, Kyoto 606-8397, Japan. E-mail amat{at}kuhp.kyoto-u.ac.jp

Background—Mast cells are multifunctional cells containing various mediators such as cytokines, proteases, and histamine. They are found in the human heart and have been implicated in ventricular hypertrophy and heart failure. However, their roles in pathogenesis of these diseases are unknown.

Methods and Results—Cultured cardiomyocytes from neonatal rats were incubated with mast cell granules (MCGs) for 24 hours. The highest concentration of diluted MCGs caused the death of 70% of cardiomyocytes. This cell death was proved to be apoptosis, as quantified by electron microscopy and biochemical criteria. MCG-mediated cytotoxicity was prevented by pretreatment of MCGs with protease inhibitors or a neutralizing antibody against rat mast cell chymase 1 (RMCP 1). RMCP 1 by itself was proved to induce cell death of cardiomyocytes. These results suggest that RMCP 1 contained in MCGs causes the death of cardiomyocytes. In contrast, MCGs induced the proliferation of intramyocardial cells other than myocytes. RMCP 1 was also proved to induce their proliferation.

Conclusions—Mast cells cause apoptosis of cardiomyocytes and proliferation of other intramyocardial cells via the activity of RMCP 1. Our results suggest that mast cell chymase may play a role in the progression of heart failure, because loss of cardiomyocytes and proliferation of nonmyocardial cells exaggerate its pathophysiology.

Key Words: cells • remodeling • heart failure • apoptosis

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