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Figure 2. Macrophage-derived FCs isolated from subcutaneous granuloma. Top, Cells isolated from subcutaneous granuloma of hypercholesterolemic rabbits are identified by immunofluorescence as macrophages (RAM-11 antibody). Inset, Negative control for immunostaining in which primary antibody (Ab) was eliminated. Middle, Intracellular lipid in macrophages from hypercholesterolemic rabbits is revealed by staining with Nile red. Inset, Nile red staining of macrophages (M{phi}) from normocholesterolemic rabbits. Bottom, Macrophage-derived FCs of granuloma express stromelysin (MMP-3). Nuclei are counterstained with bisbenzamide. Magnification x400.